Rv2172c

5,10-methylenetetrahydrofolate reductase

Validation StatusCandidate

Overview

Mechanism: enhanced NADH binding and enzymatic activity
Validation Method: mutant protein purification and enzymatic activity analysis
Drug Classes: Para-aminosalicylic acid
Organisms: Mycobacterium tuberculosis
Papers: 2
Databases: Reslit
Mutations: 2

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
L214A	-	decreased methionine production	Mycobacterium tuberculosis	Para-aminosalicylic acid	Reslit	Candidate
R159N	-	decreased methionine production	Mycobacterium tuberculosis	Para-aminosalicylic acid	Reslit	Candidate

Papers2

The T120P or M172V mutation on rv2172c confers high level para-aminosalicylic acid resistance in Mycobacterium tuberculosis.

PMID 39023395 · 2024

The study identifies T120P and M172V mutations in the rv2172c gene as novel molecular markers for high-level para-aminosalicylic acid (PAS) resistance in Mycobacterium tuberculosis. These mutations enhance NADH binding and enzymatic activity of Rv2172c, resulting in increased methionine biosynthesis and PAS resistance.

Gene Information

Gene Name: Rv2172c
Allele: rv2172c
Encodes: 5,10-methylenetetrahydrofolate reductase
Source Database: Reslit
Mechanism: enhanced NADH binding and enzymatic activity
Confers Resistance To: Para-aminosalicylic acid
Organisms Tested In: • Mycobacterium tuberculosis
Validation Method: mutant protein purification and enzymatic activity analysis

Browse AMR Genes

Rv2172c

Mutations

Gene Information