adeN

regulator

Validation StatusConfirmed2+ DBs

Overview

Mechanism: regulation of efflux pump
Validation Method: transposon insertion sequencing and flow cytometry
Drug Classes: Azithromycin, Beta-lactams, CHL, ERY, Fluoroquinolones, Fusidic acid, Nalidixic acid, RIF, Sulfonamides, TET, TMP, Fluoroquinolone, Tetracycline, Macrolide, Lincosamides, Carbapenem, Cephalosporin, Rifamycin, Diaminopyrimidines, Phenicol, Carbapenems, Delafloxacin|Gepotidacin|Omadacycline
Organisms: Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa
Geographic Location: Europe|South Korea
Papers: 5
Databases: Card DatabaseReslit
Mutations: 1

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
P41L	-	-	Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa	Delafloxacin\|Gepotidacin\|Omadacycline	Reslit	Candidate

Papers5

Fluorescence-Based Flow Sorting in Parallel with Transposon Insertion Site Sequencing Identifies Multidrug Efflux Systems in Acinetobacter baumannii.

PMID 27601573 · 2016

The study identified the AdeABC, AdeIJK, and AmvA efflux systems and their regulators, AdeRS, AdeN, and AmvR, as critical for ethidium accumulation and resistance in Acinetobacter baumannii.

Gene Information

Gene Name: adeN
Allele: adeN
Encodes: regulator
Source Database: Reslit
Mechanism: regulation of efflux pump
Organisms Tested In: • Acinetobacter baumannii
Validation Method: transposon insertion sequencing and flow cytometry
Sequence Accession: LT594095|LT594096

Evolutionary pathways to antibiotic resistance are dependent upon environmental structure and bacterial lifestyle.

PMID 31516122 · 2019

The study shows that bacterial lifestyle (planktonic vs. biofilm) influences the genetic mechanisms of antibiotic resistance. Planktonic populations developed resistance through mutations in DNA gyrase (gyrA) and topoisomerase IV (parC), while biofilm populations relied on mutations in efflux pump regulators (adeL and adeN).

Gene Information

Gene Name: adeN
Allele: adeN
Encodes: negative regulator of efflux pumps
Source Database: Reslit
Mechanism: overexpression of AdeIJK efflux pump
Confers Resistance To: AzithromycinBeta-lactamsCHLERYFluoroquinolonesFusidic acidNalidixic acidRIFSulfonamidesTETTMP
Organisms Tested In: • Acinetobacter baumannii
Validation Method: whole-population genomic sequencing and clone analysis
Sequence Accession: SAMN09783599|SAMN09783600|SAMN09783601|SAMN09783602|SAMN09783603|SAMN09783604|SAMN09783605|SAMN09783606|SAMN09783607|SAMN09783608|SAMN09783609|SAMN09783610|SAMN09783611|SAMN09783612|SAMN09783613|SAMN09783614|SAMN09783615|SAMN09783616|SAMN09783617|SAMN09783618|SAMN09783619|SAMN09783620|SAMN09783621|SAMN09783622|SAMN09783623|SAMN09783624|SAMN09783625|SAMN09783626|SAMN09783627|SAMN09783628|SAMN09783629|SAMN09783630|SAMN09783631|SAMN09783632|SAMN09783633|SAMN09783634|SAMN09783635|SAMN09783636|SAMN09783637|SAMN09783638|SAMN09783639|SAMN09783640|SAMN09783641|SAMN09783642|SAMN09783643|SAMN09783644|SAMN09783645|SAMN09783646|SAMN09783647|SAMN09783648|SAMN09783649|SAMN09783650|SAMN09783651|SAMN09783652|SAMN09783653|SAMN09783654|SAMN09783655|SAMN09783656|SAMN09783657|SAMN09783658|SAMN09783659|SAMN09783660|SAMN09783661|SAMN09783662|SAMN09783663|SAMN09783664|SAMN09783665|SAMN09783666|SAMN09783667|SAMN09783668|SAMN09783669|SAMN09783670|SAMN09783671|SAMN09783672|SAMN09783673|SAMN09783674|SAMN09783675|SAMN09783676|SAMN09783677|SAMN09783678|SAMN09783679|SAMN09783680|SAMN09783681|SAMN09783682

Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays.

PMID 33727586 · 2021

The study presents a DNA microarray-based assay for the simultaneous detection of 44 sepsis-relevant bacterial pathogens, 360 virulence factors, and 409 antibiotic resistance genes. The assay was evaluated with 14 multidrug-resistant strains, including all ESKAPE pathogens.

Gene Information

Gene Name: adeN
Allele: adeN
Encodes: efflux pump component
Source Database: Reslit
Confers Resistance To: FluoroquinoloneTetracyclineMacrolide
Organisms Tested In: • Acinetobacter baumannii
Validation Method: microarray detection

Whole-genome sequencing for the characterization of resistance mechanisms and epidemiology of colistin-resistant Acinetobacter baumannii.

PMID 35245298 · 2022

The study identified several beta-lactam resistance genes, including ADC-18, OXA-133, OXA-23, OXA-66, TEM-1, VIM-2, and RND efflux pumps (adeI, adeJ, adeK, adeN) in colistin-resistant Acinetobacter baumannii strains.

Gene Information

Gene Name: adeN
Allele: adeN
Encodes: resistance-nodulation-cell division (RND) antibiotic efflux pump
Source Database: Reslit
Confers Resistance To: MacrolideFluoroquinoloneLincosamidesCarbapenemCephalosporinTetracyclineRifamycinDiaminopyrimidinesPhenicolCarbapenems
Organisms Tested In: • Acinetobacter baumannii
Geographic Location: Europe|South Korea
Validation Method: whole-genome sequencing
Sequence Accession: GCA_001593425.2

PMID N/A

Gene Information

Gene Name: adeN
Allele: adeN
Encodes: resistance-nodulation-cell division (RND) antibiotic efflux pump
Source Database: Card Database
Mechanism: antibiotic efflux
Organisms Tested In: • Acinetobacter baumannii
Sequence Accession: KF147862.1
Protein Accession: AGV28567.1
Notes: ARO:3000559

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adeN

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