Browse AMR Genes

Explore antimicrobial resistance genes from the literature

ant(4')-IIb

aminoglycoside 4'-O-adenylyltransferase

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

Protein entries specific to ant(4')-IIb

Browse AMR rules for ant(4')-IIb

Validation StatusConfirmed2+ DBs

Overview

Mechanism: antibiotic inactivation
Validation Method: cloning in E. coli + MIC testing
Drug Classes: Tobramycin, TOBRAMYCIN, KANAMYCIN, AMIKACIN, ISEPAMICIN, Aminoglycoside
Organisms: Pseudomonas aeruginosa, Escherichia coli, E. coli, Klebsiella pneumonia, Klebsiella oxytoca, Citrobacter freundii, Enterobacter cloacae, Proteus mirabilis, Proteus vulgaris, Providencia rettgeri, Morganella morganii, Pseudomonas fluorescens, Pseudomonas putida, Acinetobacter baumannii
Geographic Location: Bulgaria, India, Iraq
Alleles: 3
Papers: 3
Databases: Card DatabaseReference Gene CatalogResFinder DatabaseReslit

Allele Variants3

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
ANT(4')-IIb	Card DatabaseReference Gene CatalogReslit	4	Tobramycin, TOBRAMYCIN +2	Pseudomonas aeruginosa +13	Bulgaria, India

Papers3

1

Aminoglycoside Resistance Gene ant(4')-IIb of Pseudomonas aeruginosa BM4492, a clinical isolate from Bulgaria.

PMID 12709326 · 2003

The ant(4')-IIb gene from Pseudomonas aeruginosa BM4492 was identified as an aminoglycoside 4'-O-adenylyltransferase that confers resistance to amikacin and tobramycin but not to dibekacin, gentamicin, or netilmicin.

Gene Information

Gene Name: ant(4')-IIb
Allele: ANT(4')-IIb
Encodes: aminoglycoside 4'-O-adenylyltransferase
Source Database: Reslit
Confers Resistance To: Tobramycin
Organisms Tested In: • Pseudomonas aeruginosa
• Escherichia coli
Geographic Location: Bulgaria
Validation Method: cloning in E. coli + MIC testing
Sequence Accession: AY114142

2

Aminoglycoside resistance gene ant(4')-IIb of Pseudomonas aeruginosa BM4492, a clinical isolate from Bulgaria.

PMID 12709326 · 2003

Gene Information

Gene Name: ant(4')-IIb
Allele: ANT(4')-IIb
Encodes: aminoglycoside nucleotidyltransferase ANT(4')-IIb
Source Database: Reference Gene Catalog
Mechanism: antibiotic inactivation
Confers Resistance To: TOBRAMYCINKANAMYCINAMIKACIN
Organisms Tested In: • Pseudomonas aeruginosa
Sequence Accession: AY114142.1
Protein Accession: AAM76670.1
Notes: ARO:3002625

3

Aminoglycoside resistance gene ant(4')-IIb of Pseudomonas aeruginosa BM4492, a clinical isolate from Bulgaria.

PMID 12709326 · 2003

Gene Information

Gene Name: ant(4')-IIb
Allele: ANT(4')-IIb
Encodes: aminoglycoside nucleotidyltransferase ANT(4')-IIb, ANT(4')
Source Database: Card Database
Mechanism: antibiotic inactivation
Confers Resistance To: TOBRAMYCINKANAMYCINAMIKACIN
Organisms Tested In: • Pseudomonas aeruginosa
Sequence Accession: AY114142.1
Protein Accession: AAM76670.1
Notes: ARO:3002625

4

Aminoglycoside resistance gene ant(4')-IIb of Pseudomonas aeruginosa BM4492, a clinical isolate from Bulgaria.

PMID 12709326 · 2003

Gene Information

Gene Name: ant(4')-IIb
Allele: ant(4')-IIb
Source Database: ResFinder Database
Mechanism: Enzymatic modification
Confers Resistance To: ISEPAMICINTOBRAMYCINAMIKACIN
Organisms Tested In: • Pseudomonas aeruginosa
Sequence Accession: AY114142

5

Spectrum of Aminoglycoside Modifying Enzymes in Gram-Negative Bacteria Causing Human Infections.

PMID 32792790 · 2020

The study identifies various aminoglycoside modifying enzymes (AMEs) in gram-negative bacteria causing human infections, including aac(6′)-lb, aac(3′)-I, aph(3′)-VI, aac(3′)-II, ant(2′)-I, ant(4′)-IIb, aph(2′)-Ib, aph(2′)-Ic, aph(2′)-Id, aac(6′)-Ie-aph(2′)-Ia, and aph(3′)-IIIa. These AMEs confer resistance to multiple aminoglycosides such as amikacin, gentamicin, tobramycin, and netilmicin.

Gene Information

Gene Name: ant(4')-IIb
Allele: ANT(4')-IIb
Encodes: aminoglycoside modifying enzyme
Source Database: Reslit
Confers Resistance To: Tobramycin
Organisms Tested In: • E. coli
• Klebsiella pneumonia
• Klebsiella oxytoca
• Citrobacter freundii
• Enterobacter cloacae
• Proteus mirabilis
• Proteus vulgaris
• Providencia rettgeri
• Morganella morganii
• Pseudomonas aeruginosa
• Pseudomonas fluorescens
• Pseudomonas putida
• Acinetobacter baumannii
Geographic Location: India
Validation Method: PCR and sequencing

6

The secrets of environmental Pseudomonas aeruginosa in slaughterhouses: Antibiogram profile, virulence, and antibiotic resistance genes.

PMID 38091178 · 2024

The study identified multiple antibiotic resistance genes in Pseudomonas aeruginosa isolates from slaughterhouses, including blaCTX-M, blaAmpC, blaSHV, blaNDM, IMP-1, aac(6')-Ib, ant(4')IIb, qnrB, tetA, mexY, TEM, and rmtC. These genes confer resistance to various antibiotics such as beta-lactams, aminoglycosides, fluoroquinolones, and tetracyclines.

Gene Information

Gene Name: ant(4')-IIb
Allele: ant(4')IIb
Encodes: Phosphorylation of aminoglycoside antibiotics
Source Database: Reslit
Mechanism: Phosphorylation of aminoglycoside antibiotics
Confers Resistance To: Aminoglycoside
Organisms Tested In: • Pseudomonas aeruginosa
Geographic Location: Iraq
Validation Method: PCR and sequencing