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aph(9)-Ia

aminoglycoside 9-phosphotransferase

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

Protein entries specific to aph(9)-Ia

Browse AMR rules for aph(9)-Ia

Validation StatusConfirmed2+ DBs

Overview

Mechanism: phosphorylation of spectinomycin
Validation Method: cloning in E. coli + MIC testing
Drug Classes: Gentamicin, SPECTINOMYCIN
Organisms: Stenotrophomonas maltophilia 142, Escherichia coli DH5α, Stenotrophomonas maltophilia 156, Stenotrophomonas maltophilia, Legionella pneumophila 130b, Legionella pneumophila 130b, Legionella pneumophila str. Lens, Legionella pneumophila str. Lens
Geographic Location: China, Lithuania
Alleles: 5
Papers: 4
Databases: Card DatabaseReference Gene CatalogResFinder DatabaseReslit

Allele Variants5

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
APH(9)-Ic	Card DatabaseReslit	2	-	Stenotrophomonas maltophilia 142 +2	China	2022	ON693243\|ON838098\|USA18493.1\|MCM2524399.1	WCI13726.1

Papers4

1

Functional characterization of a novel aminoglycoside phosphotransferase, APH(9)-Ic, and its variant from Stenotrophomonas maltophilia.

PMID 36699730 · 2022

The study identifies and characterizes a novel aminoglycoside phosphotransferase, APH(9)-Ic, and its variant, APH(9)-Ic1, from Stenotrophomonas maltophilia, which confer resistance to spectinomycin.

Gene Information

Gene Name: aph(9)-Ia
Allele: APH(9)-Ic
Encodes: aminoglycoside 9-phosphotransferase
Source Database: Reslit
Mechanism: phosphorylation of spectinomycin
Organisms Tested In: • Stenotrophomonas maltophilia 142
• Escherichia coli DH5α
Geographic Location: China
Validation Method: cloning in E. coli + MIC testing
Sequence Accession: ON693243|ON838098|USA18493.1|MCM2524399.1

2

Functional characterization of a novel aminoglycoside phosphotransferase, APH(9)-Ic, and its variant from Stenotrophomonas maltophilia.

PMID 36699730 · 2022

The study identifies and characterizes a novel aminoglycoside phosphotransferase, APH(9)-Ic, and its variant, APH(9)-Ic1, from Stenotrophomonas maltophilia, which confer resistance to spectinomycin.

Gene Information

Gene Name: aph(9)-Ia
Allele: aph(9)-Ic1
Encodes: aminoglycoside 9-phosphotransferase
Source Database: Reslit
Mechanism: phosphorylates spectinomycin
Organisms Tested In: • Stenotrophomonas maltophilia 156
• Escherichia coli DH5α
Geographic Location: China
Validation Method: cloning in E. coli + MIC testing
Sequence Accession: ON693243|ON838098|USA18493.1|MCM2524399.1

3

Stenotrophomonas maltophilia of clinical origin display higher temperature tolerance comparing with environmental isolates.

PMID 40314203 · 2025

The study identifies several AMR genes in clinical and environmental Stenotrophomonas maltophilia isolates, including beta-lactamases (bla L1, bla L2), aminoglycoside resistance genes (aph(9), aph(3), aph(6)), and a quinolone resistance gene (smqnr). Clinical isolates showed higher prevalence of these genes compared to environmental isolates.

Gene Information

Gene Name: aph(9)-Ia
Allele: aph(9)
Encodes: aminoglycoside phosphotransferase
Source Database: Reslit
Mechanism: confers resistance to aminoglycoside antibiotics
Confers Resistance To: Gentamicin
Organisms Tested In: • Stenotrophomonas maltophilia
Geographic Location: Lithuania
Validation Method: PCR and antibiotic susceptibility testing

4

Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila.

PMID 9174205 · 1997

Gene Information

Gene Name: aph(9)-Ia
Allele: APH(9)-Ia
Encodes: aminoglycoside O-phosphotransferase APH(9)-Ia
Source Database: ResFinder Database
Mechanism: Enzymatic modification
Confers Resistance To: SPECTINOMYCIN
Organisms Tested In: • Legionella pneumophila 130b
Sequence Accession: U94857.1
Protein Accession: AAB58447.1
Notes: ARO:3002662

5

Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila.

PMID 9174205 · 1997

Gene Information

Gene Name: aph(9)-Ia
Allele: APH(9)-Ia
Encodes: aminoglycoside O-phosphotransferase APH(9)-Ia
Source Database: Reference Gene Catalog
Mechanism: Enzymatic modification
Confers Resistance To: SPECTINOMYCIN
Organisms Tested In: • Legionella pneumophila 130b
Sequence Accession: U94857.1
Protein Accession: AAB58447.1
Notes: ARO:3002662

6

Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila.

PMID 9174205 · 1997

Gene Information

Gene Name: aph(9)-Ia
Allele: APH(9)-Ia
Encodes: aminoglycoside O-phosphotransferase APH(9)-Ia, APH(9)
Source Database: Card Database
Mechanism: Enzymatic modification, antibiotic inactivation
Confers Resistance To: SPECTINOMYCIN
Organisms Tested In: • Legionella pneumophila 130b
Sequence Accession: U94857.1
Protein Accession: AAB58447.1
Notes: ARO:3002662

7

Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila., Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity.

PMID 9174205, 15467720 · 1997

Gene Information

Gene Name: aph(9)-Ia
Allele: aph(9)-Ia
Source Database: ResFinder Database
Mechanism: Enzymatic modification
Confers Resistance To: SPECTINOMYCIN
Organisms Tested In: • Legionella pneumophila 130b, Legionella pneumophila str. Lens
Sequence Accession: U94857, CR628337

8

Gene Information

Gene Name: aph(9)-Ia
Allele: APH(9)-Ia
Encodes: aminoglycoside O-phosphotransferase APH(9)-Ia
Source Database: ResFinder Database
Mechanism: Enzymatic modification
Confers Resistance To: SPECTINOMYCIN
Organisms Tested In: • Legionella pneumophila str. Lens
Sequence Accession: CR628337.1
Protein Accession: CAH15775.1

9

Gene Information

Gene Name: aph(9)-Ia
Allele: APH(9)-Ia
Encodes: aminoglycoside O-phosphotransferase APH(9)-Ia
Source Database: Reference Gene Catalog
Mechanism: Enzymatic modification
Confers Resistance To: SPECTINOMYCIN
Organisms Tested In: • Legionella pneumophila str. Lens
Sequence Accession: CR628337.1
Protein Accession: CAH15775.1

10

Gene Information

Gene Name: aph(9)-Ia
Allele: APH(9)-Ic
Encodes: APH(9)
Source Database: Card Database
Mechanism: antibiotic inactivation
Organisms Tested In: • Stenotrophomonas maltophilia
Sequence Accession: ON693243.1
Protein Accession: WCI13726.1
Notes: ARO:3007539