blaIMP
metallobeta-lactamase
Overview
- Mechanism
- hydrolysis of β-lactam antibiotics
- Validation Method
- hydrolysis of imipenem and decreased susceptibility to β-lactams
- Drug Classes
- carbapenems, ampicillin, cephalothin, cefoxitin, ceftazidime, cefepime, imipenem, meropenem, aztreonam, extended-spectrum beta-lactams, piperacillin-tazobactam, penicillins, cephalosporins
- Organisms
- Pseudomonas aeruginosa, Escherichia coli, Bacteroides fragilis, Stenotrophomonas maltophilia, Bacillus cereus, Acinetobacter baumannii AC-54/97, Shigella flexneri, Acinetobacter spp., Serratia marcescens, Klebsiella pneumoniae, Alcaligenes xylosoxidans, Pseudomonas putida, Acinetobacter baumannii, Pseudomonas fluorescens
- Geographic Location
- Argentina, Brazil, Canada, Hong Kong, Italy +4 more
- Alleles
- 10
- Papers
- 16
- Databases
- Reslit
| Allele | Database | Papers | Drug Classes | Organisms | Countries | Years | Sequence Accession | Protein Accession |
|---|---|---|---|---|---|---|---|---|
| blaIMP | Reslit | 5 | carbapenems | Pseudomonas aeruginosa +6 | Japan, Italy | 1999, 2003, 2004 | AJ223604 | - |
| blaIMP-2 | Reslit | 2 | ampicillin, cephalothin +7 | Acinetobacter baumannii AC-54/97 +2 | Italy, Japan | 2000, 2004 | AJ243491 | - |
| blaIMP-3 | Reslit | 1 | ampicillin, ceftazidime +1 | Escherichia coli +1 | - | 2000 | AB010417 | - |
| blaIMP-4 | Reslit | 1 | imipenem, meropenem | Acinetobacter spp. +1 | Hong Kong | 2001 | AF244145 | - |
| blaIMP-6 | Reslit | 1 | carbapenems | Serratia marcescens +1 | - | 2001 | AB040994 | - |
| blaIMP-8 | Reslit | 1 | aztreonam, carbapenems +1 | Klebsiella pneumoniae +1 | Taiwan | 2001 | AF322577 | - |
| blaIMP-7 | Reslit | 2 | carbapenems, ceftazidime +2 | Pseudomonas aeruginosa +1 | Canada, Argentina | 2002, 2005 | AF318077 | - |
| blaIMP-10 | Reslit | 1 | carbapenems | Pseudomonas aeruginosa +1 | Japan | 2002 | AB074433|AB074434|AB074435 | - |
| blaIMP-1 | Reslit | 3 | carbapenems, imipenem +6 | Pseudomonas aeruginosa +4 | Korea, Singapore, Japan | 2003, 2004 | AY165025|AY251052|AY250709 | - |
| blaIMP-16 | Reslit | 1 | carbapenems, cephalosporins | Pseudomonas aeruginosa | Brazil | 2004 | AJ584652 | - |
Structure of In31, a blaIMP-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes.
The study characterizes the blaIMP gene, which encodes the IMP-1 metallo-beta-lactamase, and identifies several other resistance genes including aacA4, catB6, and qacG, all of which were experimentally validated for their roles in antimicrobial resistance.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP
- Encodes
- metallobeta-lactamase
- Source Database
- Reslit
- Confers Resistance To
- carbapenems
- Organisms Tested In
- • Pseudomonas aeruginosa• Escherichia coli
- Geographic Location
- Japan
- Validation Method
- hydrolysis of imipenem and decreased susceptibility to β-lactams
- Sequence Accession
- AJ223604
Carbapenem Derivatives as Potential Inhibitors of Various beta-lactamases, Including Class B Metallo-beta-lactamases.
The study identifies J-110,441 as a potent inhibitor of various beta-lactamases, including the IMP-1 metallo-beta-lactamase, which is responsible for carbapenem resistance in several bacterial species.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Confers Resistance To
- carbapenems
- Organisms Tested In
- • Pseudomonas aeruginosa• Bacteroides fragilis• Stenotrophomonas maltophilia• Bacillus cereus
- Validation Method
- enzyme inhibition assay
Characterization of the metallo-beta-lactamase determinant of Acinetobacter baumannii AC-54/97 reveals the existence of blaIMP allelic variants carried by gene cassettes of different phylogeny.
The study identifies blaIMP-2, an allelic variant of blaIMP, as a metallo-beta-lactamase gene responsible for resistance to multiple beta-lactam antibiotics in Acinetobacter baumannii AC-54/97.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-2
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Confers Resistance To
- ampicillincephalothincefoxitinceftazidimecefepimeimipenemmeropenem
- Organisms Tested In
- • Acinetobacter baumannii AC-54/97• Escherichia coli
- Geographic Location
- Italy
- Validation Method
- expression in E. coli and determination of kinetic parameters
- Sequence Accession
- AJ243491
Amino acid substitutions in a variant of IMP-1 metallo-beta-lactamase.
The study identifies the blaIMP-3 gene, a variant of the IMP-1 metallo-beta-lactamase, which confers resistance to β-lactam antibiotics through specific amino acid substitutions. The mutation at nucleotide 640 (G→A) leads to a Ser→Gly substitution at position 196, enhancing resistance to ampicillin and ceftazidime.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-3
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Mechanism
- hydrolysis of β-lactam antibiotics
- Confers Resistance To
- ampicillinceftazidimeimipenem
- Organisms Tested In
- • Escherichia coli• Shigella flexneri
- Validation Method
- cloning in E. coli + MIC testing
- Sequence Accession
- AB010417
IMP-4, a novel metallo-beta-lactamase from nosocomial Acinetobacter spp. collected in Hong Kong between 1994 and 1998.
The study identifies IMP-4, a novel metallo-beta-lactamase from Acinetobacter spp. that confers resistance to imipenem and meropenem.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-4
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Confers Resistance To
- imipenemmeropenem
- Organisms Tested In
- • Acinetobacter spp.• Escherichia coli
- Geographic Location
- Hong Kong
- Validation Method
- purification and characterization of the enzyme, MIC testing
- Sequence Accession
- AF244145
Plasmid-encoded metallo-beta-lactamase (IMP-6) conferring resistance to carbapenems, especially meropenem.
The study identifies IMP-6, a plasmid-encoded metallo-beta-lactamase that confers resistance to carbapenems, particularly meropenem, through a single point mutation resulting in an amino acid substitution (Ser-196 to Gly).
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-6
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Mechanism
- hydrolysis of carbapenems
- Confers Resistance To
- carbapenems
- Organisms Tested In
- • Serratia marcescens• Escherichia coli
- Validation Method
- purification and enzymatic activity assay
- Sequence Accession
- AB040994
Identification of a plasmid encoding SHV-12, TEM-1, and a variant of IMP-2 metallo-beta-lactamase, IMP-8, from a clinical isolate of Klebsiella pneumoniae.
The study identifies a plasmid encoding SHV-12, TEM-1, and a variant of IMP-2 metallo-beta-lactamase, IMP-8, from a clinical isolate of Klebsiella pneumoniae. IMP-8 differs from IMP-2 by four nucleotide substitutions leading to two amino acid changes and is carried on an integron-borne gene cassette.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-8
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Confers Resistance To
- aztreonamcarbapenemsextended-spectrum beta-lactams
- Organisms Tested In
- • Klebsiella pneumoniae• Escherichia coli
- Geographic Location
- Taiwan
- Validation Method
- susceptibility testing
- Sequence Accession
- AF322577
Nosocomial outbreak of carbapenem-resistant Pseudomonas aeruginosa with a new blaIMP allele, blaIMP-7.
The study identifies a new blaIMP allele, blaIMP-7, which confers resistance to carbapenems and ceftazidime in Pseudomonas aeruginosa.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-7
- Encodes
- class B beta-lactamase
- Source Database
- Reslit
- Mechanism
- hydrolyzes carbapenems and other beta-lactams
- Confers Resistance To
- carbapenemsceftazidime
- Organisms Tested In
- • Pseudomonas aeruginosa• Escherichia coli
- Geographic Location
- Canada
- Validation Method
- cloning and MIC testing
- Sequence Accession
- AF318077
Detection of a variant metallo-beta-lactamase, IMP-10, from two unrelated strains of Pseudomonas aeruginosa and an alcaligenes xylosoxidans strain.
The study identifies a variant metallo-beta-lactamase, IMP-10, which differs from IMP-1 by a single amino acid substitution (Val49 to Phe) and exhibits reduced penicillin-hydrolyzing activity.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-10
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Confers Resistance To
- carbapenems
- Organisms Tested In
- • Pseudomonas aeruginosa• Alcaligenes xylosoxidans
- Geographic Location
- Japan
- Validation Method
- cloning in E. coli + MIC testing
- Sequence Accession
- AB074433|AB074434|AB074435
Presence of Pseudomonas putida Strains Harboring Plasmids Bearing the Metallo-beta-lactamase Gene blaIMP in a Hospital in Japan.
The study identifies the presence of Pseudomonas putida strains harboring plasmids with the blaIMP gene, which confers resistance to carbapenems and cephalosporins. The blaIMP gene was transferable to Pseudomonas aeruginosa, highlighting the potential for horizontal gene transfer of carbapenem resistance.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP
- Encodes
- metalloy-beta-lactamase
- Source Database
- Reslit
- Mechanism
- hydrolyzes β-lactam antibiotics
- Confers Resistance To
- carbapenems
- Organisms Tested In
- • Pseudomonas putida• Pseudomonas aeruginosa
- Geographic Location
- Japan
- Validation Method
- PCR, conjugation, transformation, MIC testing
Evaluation of the Hodge test and the imipenem-EDTA double-disk synergy test for differentiating metallo-beta-lactamase-producing isolates of Pseudomonas spp. and Acinetobacter spp.
The study evaluated the Hodge test and imipenem-EDTA double-disk synergy test for detecting metallo-beta-lactamase (MBL)-producing isolates of Pseudomonas and Acinetobacter. It identified blaIMP-1 and blaVIM-2 as the MBL genes responsible for carbapenem resistance.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-1
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Mechanism
- hydrolyzes carbapenems
- Confers Resistance To
- carbapenems
- Organisms Tested In
- • Pseudomonas aeruginosa• Acinetobacter spp.
- Geographic Location
- Korea
- Validation Method
- PCR amplification
Molecular epidemiology of sequential outbreaks of Acinetobacter baumannii in an intensive care unit shows the emergence of carbapenem resistance.
The study identifies several AMR genes, including aacC1, aadA1a, aacA4, blaOXA-20, and blaIMP, which confer resistance to various antibiotics in carbapenem-resistant Acinetobacter baumannii strains.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP
- Encodes
- IMP-type beta-lactamase
- Source Database
- Reslit
- Mechanism
- hydrolysis
- Confers Resistance To
- carbapenems
- Organisms Tested In
- • Acinetobacter baumannii
- Geographic Location
- Italy
- Validation Method
- gene characterization
- Sequence Accession
- AY307113|AY307114
IMP-1 and a novel metallo-beta-lactamase, VIM-6, in fluorescent pseudomonads isolated in Singapore.
The study identifies blaIMP-1 and a novel metallo-beta-lactamase, VIM-6, in carbapenem-resistant Pseudomonas spp. isolates from Singapore. Both genes confer resistance to various beta-lactam antibiotics.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-1
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Confers Resistance To
- imipenemmeropenemaztreonamceftazidimecefepimepiperacillin-tazobactam
- Organisms Tested In
- • Pseudomonas putida• Pseudomonas fluorescens
- Geographic Location
- Singapore
- Validation Method
- sequencing and MIC testing
- Sequence Accession
- AY165025|AY251052|AY250709
Metallo-beta-lactamase-Producing Gram-Negative Bacilli: Laboratory-Based Surveillance in Cooperation with 13 Clinical Laboratories in the Kinki Region of Japan.
The study identified metallo-beta-lactamase (MBL) genes, including blaIMP-1, blaIMP-2, and blaVIM-2, in various gram-negative bacilli, highlighting the widespread distribution of MBL-producing organisms in the Kinki region of Japan.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Confers Resistance To
- carbapenems
- Organisms Tested In
- • Pseudomonas aeruginosa• Acinetobacter baumannii
- Geographic Location
- Japan
- Validation Method
- molecular characterization
Metallo-beta-lactamase-Producing Gram-Negative Bacilli: Laboratory-Based Surveillance in Cooperation with 13 Clinical Laboratories in the Kinki Region of Japan.
The study identified metallo-beta-lactamase (MBL) genes, including blaIMP-1, blaIMP-2, and blaVIM-2, in various gram-negative bacilli, highlighting the widespread distribution of MBL-producing organisms in the Kinki region of Japan.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-2
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Confers Resistance To
- carbapenemspenicillins
- Organisms Tested In
- • Acinetobacter spp.
- Geographic Location
- Japan
- Validation Method
- PCR and DNA sequencing
Metallo-beta-lactamase-Producing Gram-Negative Bacilli: Laboratory-Based Surveillance in Cooperation with 13 Clinical Laboratories in the Kinki Region of Japan.
The study identified metallo-beta-lactamase (MBL) genes, including blaIMP-1, blaIMP-2, and blaVIM-2, in various gram-negative bacilli, highlighting the widespread distribution of MBL-producing organisms in the Kinki region of Japan.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-1
- Encodes
- metallo-beta-lactamase
- Source Database
- Reslit
- Confers Resistance To
- carbapenemspenicillins
- Organisms Tested In
- • Acinetobacter spp.• Pseudomonas aeruginosa• Serratia marcescens
- Geographic Location
- Japan
- Validation Method
- PCR and DNA sequencing
Integron Carrying a Novel Metallo-β-Lactamase Gene, blaIMP-16, and a Fused Form of Aminoglycoside-Resistant Gene aac(6′)-30/aac(6′)-Ib′: report from the SENTRY Antimicrobial Surveillance Program.
The study identifies a novel metallo-beta-lactamase gene, blaIMP-16, and a fused form of aminoglycoside-resistant gene aac(6′)-30/aac(6′)-Ib′ in a Pseudomonas aeruginosa strain. blaIMP-16 confers resistance to carbapenems and cephalosporins, while the fused aac(6′)-30/aac(6′)-Ib′ gene confers resistance to multiple aminoglycosides.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-16
- Encodes
- metallobeta-lactamase
- Source Database
- Reslit
- Mechanism
- hydrolysis of β-lactams
- Confers Resistance To
- carbapenemscephalosporins
- Organisms Tested In
- • Pseudomonas aeruginosa
- Geographic Location
- Brazil
- Validation Method
- purification and kinetic analysis
- Sequence Accession
- AJ584652
Sensitive EDTA-Based Microbiological Assays for Detection of Metallo-β-Lactamases in Nonfermentative Gram-Negative Bacteria.
The study describes the development of a sensitive microbiological assay (EIM) for detecting metallo-β-lactamases (MBLs) in nonfermentative gram-negative bacteria. It identifies several MBL genes, including bla VIM-11a, bla IMP-7, bla SPM-1, bla L1-like, bla GOB-like, bla IND-like, and bla BcII, which confer resistance to carbapenems.
Gene Information
- Gene Name
- blaIMP
- Allele
- blaIMP-7
- Encodes
- metallobeta-lactamase
- Source Database
- Reslit
- Mechanism
- hydrolyzes carbapenems
- Confers Resistance To
- imipenemmeropenem
- Organisms Tested In
- • Pseudomonas aeruginosa
- Geographic Location
- Argentina
- Validation Method
- spectrophotometric assay and PCR
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