Browse AMR Genes

Explore antimicrobial resistance genes from the literature

blaKHM

metallo-beta-lactamase

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

Protein entries specific to blaKHM

Browse AMR rules for blaKHM

Validation StatusConfirmed2+ DBs

Overview

Mechanism: Enzymatic inactivation
Validation Method: cloning in E. coli + MIC testing
Drug Classes: Ampicillin, Ampicillin/sulbactam, Ticarcillin, Ticarcillin/clavulanic acid, Piperacillin, Ceftazidime, Cefotaxime, Cefepime, Imipenem, Meropenem, Cefoxitin, CARBAPENEM, UNKNOWN BETA-LACTAM, Carbapenem, Beta-lactams
Organisms: Citrobacter freundii, Escherichia coli, Klebsiella quasipneumoniae subsp. quasipneumoniae, Escherichia coli J53, Escherichia coli DH5α, Enterobacter hormaechei subsp. hoffmannii, Pseudomonas mosselii
Geographic Location: Japan
Alleles: 2
Papers: 4
Databases: Card DatabaseReference Gene CatalogResFinder DatabaseReslit

Allele Variants2

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
blaKHM-1	Card DatabaseReference Gene CatalogResFinder DatabaseReslit	7	Ampicillin, Ampicillin/sulbactam +13

Papers4

1

KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate.

PMID 18765691 · 2008

The study identifies KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate, which confers resistance to a wide range of β-lactam antibiotics.

Gene Information

Gene Name: blaKHM
Allele: blaKHM-1
Encodes: metallo-beta-lactamase
Source Database: Reslit
Confers Resistance To: AmpicillinAmpicillin/sulbactamTicarcillinTicarcillin/clavulanic acidPiperacillinCeftazidimeCefotaximeCefepimeImipenemMeropenemCefoxitin
Organisms Tested In: • Citrobacter freundii
• Escherichia coli
Geographic Location: Japan
Validation Method: cloning in E. coli + MIC testing
Sequence Accession: AB443628

2

KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate.

PMID 18765691 · 2008

Gene Information

Gene Name: blaKHM
Allele: blaKHM-1
Encodes: subclass B1 metallo-beta-lactamase KHM-1
Source Database: Reference Gene Catalog
Mechanism: Enzymatic inactivation
Confers Resistance To: CARBAPENEMUNKNOWN BETA-LACTAM
Organisms Tested In: • Citrobacter freundii
Sequence Accession: AB364006.1
Protein Accession: BAF91108.1
Notes: metallo beta-lactamase

3

KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate.

PMID 18765691 · 2008

Gene Information

Gene Name: blaKHM
Allele: blaKHM-1
Encodes: subclass B1 metallo-beta-lactamase KHM-1
Source Database: ResFinder Database
Mechanism: Enzymatic inactivation
Confers Resistance To: CARBAPENEMUNKNOWN BETA-LACTAM
Organisms Tested In: • Citrobacter freundii
Sequence Accession: AB364006.1
Protein Accession: BAF91108.1
Notes: metallo beta-lactamase

4

KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate.

PMID 18765691 · 2008

Gene Information

Gene Name: blaKHM
Allele: blaKHM-1
Encodes: subclass B1 metallo-beta-lactamase KHM-1, KHM beta-lactamase
Source Database: Card Database
Mechanism: Enzymatic inactivation, antibiotic inactivation
Confers Resistance To: CARBAPENEMUNKNOWN BETA-LACTAM
Organisms Tested In: • Citrobacter freundii
Sequence Accession: AB364006.1
Protein Accession: BAF91108.1
Notes: metallo beta-lactamase, ARO:3000847

5

Multiple β-Lactam Resistance Gene-Carrying Plasmid Harbored by Klebsiella quasipneumoniae Isolated from Urban Sewage in Japan.

PMID 31554719 · 2019

The study identifies a plasmid carrying four beta-lactamase genes, including the rare carbapenemase KHM-1, in a carbapenem-resistant Klebsiella quasipneumoniae isolate from urban sewage in Japan. The plasmid pTMSNI47-1 was found to confer broad-spectrum β-lactam resistance through the cooperative effects of its beta-lactamase genes.

Gene Information

Gene Name: blaKHM
Allele: blaKHM-1
Encodes: metallo-beta-lactamase
Source Database: Reslit
Confers Resistance To: CarbapenemBeta-lactams
Organisms Tested In: • Klebsiella quasipneumoniae subsp. quasipneumoniae
• Escherichia coli J53
• Escherichia coli DH5α
Geographic Location: Japan
Validation Method: transformation and MIC testing
Sequence Accession: AP019687|AP019688|AP019689|AP019690|AP019691

6

Molecular characterization of blaKHM-1 encoding plasmid in an Enterobacter hormaechei subsp. hoffmannii isolate from blood culture.

PMID 31929575 · 2020

The study characterizes the blaKHM-1 gene, a metallo-beta-lactamase, located on a plasmid in an Enterobacter hormaechei subsp. hoffmannii isolate, highlighting its role in carbapenem resistance and potential for horizontal gene transfer.

Gene Information

Gene Name: blaKHM
Allele: blaKHM-1
Encodes: metallo-beta-lactamase
Source Database: Reslit
Mechanism: hydrolysis of β-lactam antibiotics
Confers Resistance To: Carbapenem
Organisms Tested In: • Enterobacter hormaechei subsp. hoffmannii
• Escherichia coli
Geographic Location: Japan
Validation Method: PCR and Carba NP test
Sequence Accession: AP019817|AP019818|AP019819|AP019820|AP019821

7

PMID unpublished

Gene Information

Gene Name: blaKHM
Allele: blaKHM-1
Source Database: ResFinder Database
Mechanism: Enzymatic inactivation
Confers Resistance To: UNKNOWN BETA-LACTAM
Organisms Tested In: • Citrobacter freundii
Sequence Accession: AB364006
Notes: metallo beta-lactamase

8

Gene Information

Gene Name: blaKHM
Allele: blaKHM-2
Encodes: subclass B1 metallo-beta-lactamase KHM-2, KHM beta-lactamase
Source Database: Card Database
Mechanism: antibiotic inactivation
Confers Resistance To: CARBAPENEM
Organisms Tested In: • Pseudomonas mosselii
Sequence Accession: OQ646729.1
Protein Accession: WEY36412.1
Notes: ARO:3008228

9

Gene Information

Gene Name: blaKHM
Allele: blaKHM-2
Encodes: subclass B1 metallo-beta-lactamase KHM-2
Source Database: Reference Gene Catalog
Mechanism: antibiotic inactivation
Confers Resistance To: CARBAPENEM
Organisms Tested In: • Pseudomonas mosselii
Sequence Accession: OQ646729.1
Protein Accession: WEY36412.1
Notes: ARO:3008228