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blaPEN-I

PEN-I family class A beta-lactamase

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

Protein entries specific to blaPEN-I

Browse AMR rules for blaPEN-I

Validation StatusConfirmed2+ DBs

Overview

Mechanism: hydrolysis of β-lactam antibiotics
Validation Method: expression in E. coli and susceptibility testing
Drug Classes: BETA-LACTAM, Ampicillin, Cephalothin, Ceftazidime, Cefepime
Organisms: Burkholderia pseudomallei, Escherichia coli
Alleles: 3
Papers: 4
Databases: Reference Gene CatalogReslit

Allele Variants3

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
blaPEN-I	Reference Gene Catalog	1	BETA-LACTAM	Burkholderia pseudomallei	-	2003	AY032873.1	AAK52327.1
bla_PenI	Reslit

Papers4

1

Burkholderia pseudomallei class a beta-lactamase mutations that confer selective resistance against ceftazidime or clavulanic acid inhibition.

PMID 12821450 · 2003

Gene Information

Gene Name: blaPEN-I
Allele: blaPEN-I
Encodes: PEN-I family class A beta-lactamase
Source Database: Reference Gene Catalog
Confers Resistance To: BETA-LACTAM
Organisms Tested In: • Burkholderia pseudomallei
Sequence Accession: AY032873.1
Protein Accession: AAK52327.1

2

Insights into beta-lactamases from Burkholderia Species, Two Phylogenetically Related yet Distinct Resistance Determinants.

PMID 23658015 · 2013

The study identifies and characterizes two beta-lactamases, PenA from B. multivorans and PenI from B. pseudomallei, which show distinct resistance profiles against various β-lactam antibiotics.

Gene Information

Gene Name: blaPEN-I
Allele: bla_PenI
Encodes: beta-lactamase
Source Database: Reslit
Mechanism: hydrolysis of β-lactam antibiotics
Confers Resistance To: AmpicillinCephalothin
Organisms Tested In: • Escherichia coli
• Burkholderia pseudomallei
Validation Method: expression in E. coli and susceptibility testing
Sequence Accession: 3W4Q|3W4P|3W4O

3

Exposing a beta-lactamase "Twist": the Mechanistic Basis for the High Level of Ceftazidime Resistance in the C69F Variant of the Burkholderia pseudomallei PenI beta-lactamase.

PMID 26596949 · 2016

The C69F variant of the PenI beta-lactamase from Burkholderia pseudomallei confers high-level ceftazidime resistance through a conformational change that enhances catalytic efficiency.

Gene Information

Gene Name: blaPEN-I
Allele: blaPenI
Encodes: beta-lactamase
Source Database: Reslit
Mechanism: The C69F variant of the Burkholderia pseudomallei PenI beta-lactamase exhibits a branched kinetic mechanism for ceftazidime hydrolysis, suggesting there are two different conformations of the enzyme.
Confers Resistance To: Ceftazidime
Organisms Tested In: • Burkholderia pseudomallei
• Escherichia coli
Validation Method: expression in E. coli and determination of MIC values

4

Burkholderia pseudomallei PenI beta-lactamase and variants are potently inhibited by taniborbactam.

PMID 40938351 · 2025

Taniborbactam effectively inhibits the beta-lactamase PenI and its variants C69Y and P167S, reducing the MIC of cefepime in E. coli strains expressing these enzymes.

Gene Information

Gene Name: blaPEN-I
Allele: blaPenI
Encodes: beta-lactamase
Source Database: Reslit
Mechanism: inhibition by taniborbactam
Confers Resistance To: CeftazidimeCefepime
Organisms Tested In: • Escherichia coli
• Burkholderia pseudomallei
Validation Method: MIC testing in isogenic E. coli strains