The study identifies SME-1 and SME-2, carbapenem-hydrolyzing class A beta-lactamases from geographically diverse Serratia marcescens strains, which confer resistance to imipenem, meropenem, aztreonam, and cefoxacin.

The study identifies the blaSME-1 gene as a beta-lactamase that imposes a significant fitness cost on Escherichia coli, affecting growth, culture viability, and plasmid stability. The fitness cost is associated with the SME-1 protein and its signal sequence, but not with its enzymatic activity.

The study identifies various carbapenem resistance mechanisms in pediatric Enterobacteriaceae, including plasmid-mediated beta-lactamases (CTX-M, CMY, KPC, IMP), chromosomal beta-lactamase (SME), and porin alterations (ompK35 and ompK36).

Ceftaroline-avibactam showed potent activity against β-lactamase-producing Enterobacteriaceae, including those with multiple β-lactamases, and was effective against KPC-producing strains. It had limited activity against Acinetobacter spp. and P. aeruginosa.

Ceftaroline-avibactam showed potent activity against β-lactamase-producing Enterobacteriaceae, including those with multiple β-lactamases, and was effective against KPC-producing strains. It had limited activity against Acinetobacter spp. and P. aeruginosa.

Substitutions in the Mycobacterium tuberculosis β-lactamase BlaC, particularly R220A, R220S, A244R, S130G, T237A, and T237S, were found to reduce the effectiveness of clavulanate, although the wild-type BlaC remains susceptible to ampicillin-clavulanate.

The study identified bla KPC, bla SME, and bla NDM-1 as the primary carbapenemase-encoding genes in carbapenem-resistant Enterobacteriaceae (CRE) isolates, with bla KPC being the most common. These genes conferred resistance to carbapenems such as meropenem and imipenem.

The study identified various carbapenemase genes, including blaKPC, blaSME, blaOXA-48, blaNDM, and blaNMC, in carbapenem-non-susceptible Enterobacteriaceae isolates in Quebec, Canada. These genes were associated with resistance to carbapenems and other antibiotics.

The paper characterizes various class A carbapenemases, including KPC, GES, SME, NmcA, FRI-1, BIC-1, SFC-1, and PenA, highlighting their roles in conferring resistance to carbapenems and other β-lactam antibiotics.

The paper characterizes various class A carbapenemases, including KPC, GES, SME, NmcA, FRI-1, BIC-1, SFC-1, and PenA, highlighting their roles in conferring resistance to carbapenems and other β-lactam antibiotics.

The study evaluates 11 phenotypic assays for the detection of carbapenemase-producing Enterobacteriaceae (CPE) and identifies the effectiveness of various assays in detecting different types of carbapenemase genes, including bla KPC, bla NDM, bla OXA-48-type, bla VIM, bla IMP, bla SME, and bla IMI.

The study identified various carbapenemase genes, including bla OXA-48 like, bla KPC, bla NDM, bla SME, bla IMP, and bla VIM, contributing to carbapenem resistance in Enterobacteriaceae isolates. These genes were found in different bacterial species, and their presence influenced the susceptibility profiles of the isolates to newer β-lactam/β-lactamase inhibitors.

The study evaluates the effectiveness of imipenem-relebactam and meropenem-vaborbactam against carbapenem-resistant Serratia marcescens strains producing the SME-4 carbapenemase.

The study presents a novel assay called FIBA for the rapid detection and classification of bacterial carbapenemases. It identifies various carbapenemase types including KPC, SME, NMC-A, NDM, VIM, IMP, and OXA, demonstrating high sensitivity and specificity.

The study evaluates the NG-Test® CARBA 5 assay for detecting carbapenemases in Enterobacterales, showing high sensitivity and specificity. It identifies bla KPC, bla OXA-48-like, bla NDM, bla VIM, bla IMP, bla IMI, and bla SME as the main carbapenemase genes detected.

Disruption of DsbA-mediated disulfide bond formation incapacitates diverse β-lactamases and destabilizes mobile colistin resistance enzymes. Chemical inhibition of DsbA sensitizes multidrug-resistant clinical isolates to existing antibiotics.

The study identified multiple antimicrobial resistance (AMR) genes and mutations in the Serratia marcescens complex, highlighting the presence of hospital-adapted lineages with a high prevalence of multidrug-resistant (MDR) strains. Key AMR genes include blaCTX-M, blaNDM, blaOXA, qnrS1, tet(A), aac(6')-Ib, mph(A), erm(B), aadA, floR, sul1, and dfrA12, which confer resistance to various antibiotics such as beta-lactams, fluoroquinolones, tetracyclines, aminoglycosides, macrolides, florfenicol, sulfonamides, and trimethoprim.

The study characterizes various carbapenem resistance genes such as blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP, blaGES, blaSIM, blaTEM, blaSHV, blaCTX-M, ampC, mecA, vanA, vanB, vanC, mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 in Enterobacterales and other bacterial species, highlighting their role in carbapenem resistance and horizontal gene transfer.

The study characterizes various carbapenem resistance genes such as blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP, blaGES, blaSIM, blaTEM, blaSHV, blaCTX-M, ampC, mecA, vanA, vanB, vanC, mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 in Enterobacterales and other bacterial species, highlighting their role in carbapenem resistance and horizontal gene transfer.

The study characterizes various carbapenem resistance genes such as blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP, blaGES, blaSIM, blaTEM, blaSHV, blaCTX-M, ampC, mecA, vanA, vanB, vanC, mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 in Enterobacterales and other bacterial species, highlighting their role in carbapenem resistance and horizontal gene transfer.

The study identifies specific mutations in the SME-1 beta-lactamase that contribute to its carbapenemase activity, enhancing resistance to carbapenems.

The EasyScreen™ ESBL/CPO Detection Kit effectively detects various β-lactam resistance genes, including bla VIM, bla NDM, bla IMP, bla OXA-48, bla KPC, bla OXA-23, bla OXA-51, bla SME, bla IMI, bla GES, bla TEM, bla SHV, bla CTX-M, bla CMY, bla DHA, and the mcr-1 gene, demonstrating high sensitivity and specificity for carbapenemase and ESBL detection in Enterobacterales, Pseudomonas spp., and Acinetobacter spp.

The study characterizes the impact of minor carbapenemases (SME-1, NmcA, IMI-1, and FRI-1) on susceptibility to imipenem-relebactam, meropenem-vaborbactam, and cefiderocol in Enterobacterales. It shows that these carbapenemases significantly reduce susceptibility to imipenem-relebactam but not to meropenem-vaborbactam or cefiderocol.

The study identified that various carbapenemase genes, including blaKPC-3, blaSME-2, blaIMP-4, blaNDM-1, blaVIM-27, blaCMY-10, and blaOXA-48, confer a meropenem inoculum effect when expressed in E. coli K-12. These genes were experimentally validated to show increased resistance with higher inocula.

The study identified several carbapenemase genes, including bla NDM-1, bla NDM-5, bla KPC-2, bla OXA-23, bla SME, and bla IMI, as well as extended-spectrum beta-lactamase genes such as bla CTX-M, bla SHV, and bla TEM, and AmpC beta-lactamase gene bla DHA, which contribute to carbapenem resistance in CRKP isolates from Shanxi Province.

The study reports the first isolation of SME-2-producing S. marcescens in New Zealand, highlighting its resistance to carbapenems and the presence of additional resistance mechanisms such as aac(6')-Ic.

The study identifies and characterizes several L2 beta-lactamase variants (L2a, L2b, L2c, L2d) along with SME-1 and KPC-2, highlighting their structural and functional dynamics in relation to β-lactam resistance.

The study identifies a novel carbapenemase, SME-6, in a Serratia ureilytica isolate from Germany, which shows temperature-dependent resistance to carbapenems.

The study evaluated the performance of bioMérieux Advanced Reporting Tool (bioART) expert rules for identifying carbapenemase classes in Enterobacterales isolates using the VITEK2 platform. It found that the bioART rules, when combined with the VITEK2 Advanced Expert System (AES), improved the detection of carbapenemase-producing isolates, particularly for KPC, MBL, and OXA-48-like enzymes.

The study identified that various carbapenemase genes, including blaKPC-3, blaSME-2, blaIMP-4, blaNDM-1, blaVIM-27, blaCMY-10, and blaOXA-48, when expressed in E. coli, significantly increased meropenem MICs and exhibited an inoculum effect. These findings highlight the role of carbapenemases in carbapenem resistance and the importance of the inoculum effect in diagnosing carbapenemase-producing CRE.

The study identified and characterized the blaSme-1 gene, which encodes a carbapenem-hydrolyzing class A beta-lactamase from Serratia marcescens S6.