blaTEM-1

beta-lactamase

Overview

Mechanism: prevents irreversible cross-linking of the inhibitor to Ser130
Drug Classes: Penicillin, Ceftazidime, Cefotaxime, Ampicillin, Clavulanic acid, Sulbactam, Tazobactam, Cefixime, Amoxicillin, Amoxicillin clavulanic acid
Organisms: Escherichia coli, E. coli, Klebsiella pneumoniae
Papers: 7
Mutations: 13

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
S130G	-	prevents irreversible cross-linking of the inhibitor to Ser130, confers resistance to β-lactam-clavulanate combinations	Escherichia coli, E. coli	penicillinampicillinclavulanic acid+2 more	Reslit	Candidate
R164S	-	-	E. coli	ceftazidimecefotaxime	Reslit	Candidate
E104K	-	-	E. coli	ceftazidimecefotaximeampicillin	Reslit	Candidate
G238S	-	-	E. coli	ceftazidimecefotaxime	Reslit	Candidate
S268G	-	-	E. coli	ceftazidime	Reslit	Candidate
S164F	-	-	-	ampicillinpenicillin	Reslit	Candidate
R244C	-	confers resistance to β-lactam-clavulanate combinations	E. coli	ampicillinclavulanic acidsulbactam+1 more	Reslit	Candidate
R244S	-	confers resistance to β-lactam-clavulanate combinations	E. coli	ampicillinclavulanic acidsulbactam+1 more	Reslit	Candidate
N276D	-	confers resistance to β-lactam-clavulanate combinations	E. coli	ampicillinclavulanic acidsulbactam+1 more	Reslit	Candidate
M69L	-	confers resistance to β-lactam-clavulanate combinations	E. coli	ampicillinclavulanic acidsulbactam+1 more	Reslit	Candidate
A237R	-	-	E. coli	cefixime	Reslit	Candidate
A237K	-	-	E. coli	cefixime	Reslit	Candidate
-	G175A	-	Escherichia coli, Klebsiella pneumoniae	amoxicillinamoxicillin clavulanic acid	Reslit	Candidate

Papers7

Structural consequences of the inhibitor-resistant Ser130Gly substitution in TEM beta-lactamase.

Research Article

PMID 15981999 · 2005

The Ser130Gly substitution prevents irreversible cross-linking of the inhibitor to Ser130, leading to resistance.

Mutation Information

blaTEM-1: p.S130G

Protein Change: S130G
Organisms: Escherichia coli
Confers Resistance To: penicillin
Effect on Function: prevents irreversible cross-linking of the inhibitor to Ser130
Validation Method: structural analysis and kinetic measurements
Source Database: Reslit

Construction and characterization of mutants of the TEM-1 beta-lactamase containing amino acid substitutions associated with both extended-spectrum resistance and resistance to beta-lactamase inhibitors.

Research Article

PMID 10428907 · 1999

These mutations were introduced into the TEM-1 beta-lactamase to study their effects on resistance to beta-lactam antibiotics and beta-lactamase inhibitors.

Mutation Information

blaTEM-1: p.R164S blaTEM-1: p.E104K blaTEM-1: p.G238S

Protein Change: R164S, E104K, G238S
Organisms: E. coli
Confers Resistance To: ceftazidimecefotaxime
Validation Method: MIC testing
Source Database: Reslit

Mutant TEM beta-lactamase producing resistance to ceftazidime, ampicillins, and beta-lactamase inhibitors.

Research Article

PMID 11850243 · 2002

The mutations were identified through DNA shuffling and mutagenesis experiments.

Mutation Information

blaTEM-1: p.E104K blaTEM-1: p.S268G

Protein Change: E104K, S268G
Organisms: E. coli
Confers Resistance To: ceftazidimeampicillin
Validation Method: experimental validation through DNA shuffling and mutagenesis
Source Database: Reslit

Antibiotic resistance in Pakistan: a systematic review of past decade.

Review

PMID 33676421 · 2021

Mutation Information

blaTEM-1: p.S164F

Protein Change: S164F
Confers Resistance To: ampicillinpenicillin
Source Database: Reslit

Selection and characterization of beta-lactam-beta-lactamase inactivator-resistant mutants following PCR mutagenesis of the TEM-1 beta-lactamase gene.

Research Article

PMID 9660980 · 1998

Mutant enzymes with replacements of Met69, Arg244, and Asn276 had been reported to confer resistance to beta-lactam-clavulanate combinations.

Mutation Information

blaTEM-1: p.S130G blaTEM-1: p.R244C blaTEM-1: p.R244S blaTEM-1: p.N276D blaTEM-1: p.M69L

Protein Change: S130G, R244C, R244S, N276D, M69L
Organisms: E. coli
Confers Resistance To: ampicillinclavulanic acidsulbactamtazobactam
Effect on Function: confers resistance to β-lactam-clavulanate combinations
Validation Method: MIC testing
Source Database: Reslit

Covalent docking and molecular dynamics simulations reveal the specificity-shifting mutations Ala237Arg and Ala237Lys in TEM beta-lactamase.

Research Article

PMID 35759512 · 2022

Both mutations enhance resistance to cefixime by forming salt bridges with the drug's negatively-charged R-group.

Mutation Information

blaTEM-1: p.A237R blaTEM-1: p.A237K

Protein Change: A237R, A237K
Organisms: E. coli
Confers Resistance To: cefixime
Validation Method: minimum inhibitory concentration assays
Source Database: Reslit

Predicting antimicrobial susceptibilities for Escherichia coli and Klebsiella pneumoniae isolates using whole genomic sequence data.

Research Article

PMID 23722448 · 2013

associated with increased beta-lactamase production

Mutation Information

blaTEM-1: c.G175A

Nucleotide Change: G175A
Organisms: Escherichia coli, Klebsiella pneumoniae
Confers Resistance To: amoxicillinamoxicillin clavulanic acid
Validation Method: whole-genome sequencing and phenotypic testing
Source Database: Reslit

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
S130G	-	prevents irreversible cross-linking of the inhibitor to Ser130, confers resistance to β-lactam-clavulanate combinations	Escherichia coli, E. coli	penicillinampicillinclavulanic acid+2 more	Reslit	Candidate
R164S	-	-	E. coli	ceftazidimecefotaxime	Reslit	Candidate
E104K	-	-	E. coli	ceftazidimecefotaximeampicillin	Reslit	Candidate
G238S	-	-	E. coli	ceftazidimecefotaxime	Reslit	Candidate
S268G	-	-	E. coli	ceftazidime	Reslit	Candidate
S164F	-	-	-	ampicillinpenicillin	Reslit	Candidate
R244C	-	confers resistance to β-lactam-clavulanate combinations	E. coli	ampicillinclavulanic acidsulbactam+1 more	Reslit	Candidate
R244S	-	confers resistance to β-lactam-clavulanate combinations	E. coli	ampicillinclavulanic acidsulbactam+1 more	Reslit	Candidate
N276D	-	confers resistance to β-lactam-clavulanate combinations	E. coli	ampicillinclavulanic acidsulbactam+1 more	Reslit	Candidate
M69L	-	confers resistance to β-lactam-clavulanate combinations	E. coli	ampicillinclavulanic acidsulbactam+1 more	Reslit	Candidate
A237R	-	-	E. coli	cefixime	Reslit	Candidate
A237K	-	-	E. coli	cefixime	Reslit	Candidate
-	G175A	-	Escherichia coli, Klebsiella pneumoniae	amoxicillinamoxicillin clavulanic acid	Reslit	Candidate

blaTEM-1

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blaTEM-1

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