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bpeB

RND efflux pump permease component

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

Protein entries specific to bpeB

Browse AMR rules for bpeB

Validation StatusSupported

Overview

Mechanism: substrate-specific efflux
Validation Method: RT-qPCR analysis
Drug Classes: Chloramphenicol, Trimethoprim-sulfamethoxazole, Erythromycin, Clindamycin, Tetracycline, Levofloxacin, Trimethoprim, Acriflavine, Ethidium bromide, Erythromycin|Clindamycin
Organisms: Burkholderia pseudomallei, Escherichia coli
Geographic Location: Europe|Malaysia, Malaysian Borneo
Alleles: 2
Papers: 3
Databases: Reslit
Mutations: 6

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
S132A	-	-	Escherichia coli	Erythromycin\|Clindamycin	Reslit	Candidate
S133A	-	-	Escherichia coli	Erythromycin\|Clindamycin	Reslit	Candidate
S134D	-	-	Escherichia coli	Erythromycin\|Clindamycin

Allele Variants2

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
bpeB	Reslit	2	Chloramphenicol, Trimethoprim-sulfamethoxazole +7	Burkholderia pseudomallei +1	Europe\|Malaysia, Malaysian Borneo	2021, 2026	PRJNA526444	-
BpeB	Reslit	1	-	Burkholderia pseudomallei +1	-	2023	7WLS\|7WLV	-

Papers3

1

Genomic and RT-qPCR analysis of trimethoprim-sulfamethoxazole and meropenem resistance in Burkholderia pseudomallei clinical isolates.

PMID 33592059 · 2021

The study identifies a mutation in the amrR gene, a regulator of the AmrAB-OprA efflux pump, which leads to meropenem resistance in Burkholderia pseudomallei. This mutation was experimentally validated through genomic analysis and RT-qPCR.

Gene Information

Gene Name: bpeB
Allele: bpeB
Encodes: RND efflux pump permease component
Source Database: Reslit
Confers Resistance To: ChloramphenicolTrimethoprim-sulfamethoxazole
Organisms Tested In: • Burkholderia pseudomallei
Geographic Location: Europe|Malaysia
Validation Method: RT-qPCR analysis
Sequence Accession: PRJNA526444

2

Crystal structures of multidrug efflux transporters from Burkholderia pseudomallei suggest details of transport mechanism.

PMID 37428905 · 2023

The study characterizes the multidrug efflux transporters BpeB and BpeF from Burkholderia pseudomallei, demonstrating their ability to confer resistance to detergents through experimental validation via MIC testing.

Gene Information

Gene Name: bpeB
Allele: BpeB
Encodes: multidrug efflux transporter
Source Database: Reslit
Organisms Tested In: • Burkholderia pseudomallei
• Escherichia coli
Validation Method: MIC testing of substrates in E. coli expressing BpeB
Sequence Accession: 7WLS|7WLV

3

Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate-binding pocket.

PMID 41390959 · 2026

The hydrophilic patch in the substrate-binding pocket of BpeB and BpeF influences their substrate specificity, with mutations in this region altering resistance profiles to various antibiotics.

Gene Information

Gene Name: bpeB
Allele: bpeB
Encodes: RND-type transporter
Source Database: Reslit
Mechanism: substrate-specific efflux
Confers Resistance To: ErythromycinClindamycinTetracyclineLevofloxacinTrimethoprimAcriflavineEthidium bromide
Organisms Tested In: • Escherichia coli
• Burkholderia pseudomallei
Geographic Location: Malaysian Borneo
Validation Method: drug susceptibility agar plate assay