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Explore antimicrobial resistance genes from the literature
Explore antimicrobial resistance genes from the literature
trimethoprim-resistant dihydrofolate reductase DfrA5
Overview
| Allele | Database | Papers | Drug Classes | Organisms | Countries | Years | Sequence Accession | Protein Accession |
|---|---|---|---|---|---|---|---|---|
| DfrA5 | Card DatabaseReference Gene CatalogReslit | 80 | TRIMETHOPRIM, Trimethoprim +2 | Escherichia coli +30 | India, Sweden, Australia, Global, Nebraska|United States, Nepal, Norway, San Francisco|Seattle|Minnesota|Minneapolis|Sacramento|Dallas|Jackson, Kenya, Germany, UK|Latin America, South Asia|sub-Saharan Africa|The Gambia|Mali|Kenya|Mozambique|Bangladesh|India|Pakistan, Europe, Italy, Ecuador, South Africa, United Kingdom, China, Brazil, Czech Republic, Denmark|Finland|Iceland|Lithuania|Netherlands|Spain, Armenia|Georgia, Pacific region|Hawaii, Greece, Spain, Canada, Russia|Moscow, Bangladesh, Northern Ireland, South Australia|Australia, North Sea|Baltic Sea, Portugal|Antarctic|South Shetland Islands|North Adriatic Sea|Bohai Sea|Adriatic Sea|Pacific Ocean|Baltic Sea, Thailand, Oxfordshire, UK, Belgium|The Netherlands|Europe, Western Africa|Mali, Kuwait, Mozambique, Oman, Iran, Central Adriatic|Central Adriatic Sea, Egypt, UK, Baton Rouge, LA, Croatia, Ethiopia, Uruguay|Chile, Portugal|various regions, Armenia, Austria, Somali region, Ethiopia|Somali Region, Ethiopia, Europe|Africa|North America|South America|Asia|Oceania, New Mexico, USA, Australia|France|Bangladesh|United States, Saudi Arabia, northwest China|China | 2002, 2003, 2006, 2010, 2014, 2015, 2017, 2018, 2019, 2020, 2021, 2022, 2023, 2024, 2025 | AB188269.1 | BAD99255.1 |
| dfr A5 | Reslit | 3 | Trimethoprim | Escherichia coli +2 | Edo state, Nigeria, Italy, Germany | 2022, 2023, 2024 | JAIKTX000000000|JAIKUW000000000|JAIKTX010000000|JAIKUW010000000 | - |
| dfrA5/qacEdelta | Reslit | 1 | Trimethoprim | - | South Australia|New South Wales | 2024 | OR095837|OR095838|OR095839|OR095840|OR095841|OR095842|OR095843|OR095845|OR095846|OR095847|OR095848|OR095849|OR095850|OR095851|OR095852|OR095853|OR095854|OR095855|OR095856 | - |
| dfrA5 | Card DatabaseResFinder Database | 2 | TRIMETHOPRIM | Acinetobacter baumannii, Escherichia coli, uncultured bacterium, Plasmid pLMO20 +1 | - | 2010 | KF030794, FJ001870, AY139589, X12868 | ABB89122.1 |
Occurrence of antibiotic resistance gene cassettes aac(6')-Ib, dfrA5, dfrA12, and ereA2 in class I integrons in non-O1, non-O139 Vibrio cholerae strains in India.
Occurrence of antibiotic resistance gene cassettes aac(6')-Ib, dfrA5, dfrA12, and ereA2 in class I integrons in non-O1, non-O139 Vibrio cholerae strains in India.
Incidence of class 1 integrons in multiple antibiotic-resistant Gram-negative copiotrophic bacteria from the River Torsa in India
The study identified various gene cassettes within class 1 integrons in multiple antibiotic-resistant Gram-negative bacteria from the River Torsa in India, including dfrA1, dfrA5, dfrA7, dfrA12, dfrA17, aac(6')-Ib, aadA1, aadA6, and a novel ORF with homology to dfrA1.
Occurrence of integron-associated resistance gene cassettes located on antibiotic resistance plasmids isolated from a wastewater treatment plant.
Molecular characterisation of trimethoprim resistance in Escherichia coli and Klebsiella pneumoniae during a two year intervention on trimethoprim use.
The study identified various dfr genes, including dfrA1, dfrA17, dfrA5, dfrA7, dfrA8, dfrA12, dfrA14, dfrA24, and dfrA26, as major contributors to trimethoprim resistance in E. coli and K. pneumoniae. The distribution of these genes remained largely unchanged during the intervention period.
Distribution of class 1 integrons with IS26-mediated deletions in their 3'-conserved segments in Escherichia coli of human and animal origin.
The study identifies a unique dfrA5-IS26 configuration in class 1 integrons of E. coli strains from both human and animal origins, highlighting the role of IS26 in modifying integron structures and facilitating the spread of antibiotic resistance genes.
Antimicrobial Resistance in Bacteria: Mechanisms and Current Challenges
This paper characterizes several beta-lactamases, including TEM-1, SHV-1, CTX-M-15, and NDM-1, which confer resistance to various beta-lactam antibiotics. It also identifies erm(B) and mef(A) as mechanisms of macrolide, lincosamide, and streptogramin B resistance. Additionally, aadA1 and aac(6')-Ib are noted for aminoglycoside resistance, while catA1 and floR contribute to chloramphenicol resistance. The vanA gene is associated with glycopeptide resistance, and mcr-1 is linked to polymyxin resistance.
Within-population distribution of trimethoprim resistance in Escherichia coli before and after a community-wide intervention on trimethoprim use.
The study identified dfrA1, dfrA5, and dfrA17 as trimethoprim resistance genes in Escherichia coli, showing their distribution across different genetic subpopulations and changes in resistance frequencies after a community-wide intervention on trimethoprim use.
Occurrence of Antimicrobial-Resistant Escherichia coli and Salmonella enterica in the Beef Cattle Production and Processing Continuum.
The study identified several AMR genes in E. coli and Salmonella, including beta-lactamase genes (blaCMY, blaCTX-M), sulfonamide resistance genes (sul1, sul2, sul3), and dihydrofolate reductase genes (dfrA1, dfrA5, dfrA7, dfrA12). These genes conferred resistance to various antibiotics such as ampicillin, ceftiofur, ceftriaxone, and trimethoprim-sulfamethoxazole.
Phylogenetic distribution and prevalence of genes encoding class I Integrons and CTX-M-15 extended-spectrum β-lactamases in Escherichia coli isolates from healthy humans in Chandigarh, India.
The study identifies the presence of class 1 integrons and bla CTX-M-15 genes in commensal E. coli isolates from healthy individuals in Chandigarh, India. It also detects several gene cassettes conferring resistance to trimethoprim, streptomycin, spectinomycin, streptothricin, chloramphenicol, tetracycline, and sulfonamides.
Clinical epidemiology and molecular analysis of extended-spectrum-β-lactamase-producing Escherichia coli in Nepal: characteristics of sequence types 131 and 648.
The study identified bla CTX-M-15, aac (3)-IIa, aadA1, aadA2, aadA5, qnrB4, qnrS1, aac (6')-Ib-cr, bla OXA-1, bla TEM-1B, bla SHV-12, bla CMY-42, bla DHA-1, mphA, ermB, catA1, catB3, sul1, sul2, dfrA12, dfrA17, dfrA1, dfrA5, tetA, tetB, and tetD as key AMR genes in ESBL-producing E. coli isolates in Nepal, highlighting the prevalence of multidrug resistance.
Comparative genomics of non-pseudomonal bacterial species colonising paediatric cystic fibrosis patients.
The study analyzed non-pseudomonal bacterial species from pediatric cystic fibrosis patients, identifying several AMR genes and mutations. Key findings include the presence of beta-lactamase genes (blaZ, blaI, blaRI) in Staphylococcus aureus, fosfomycin resistance gene fosB, fusidic acid resistance gene fusC, and efflux pumps smeZ, smeJ, and smeK in Stenotrophomonas maltophilia. Additionally, Enterobacter cloacae isolates carried resistance genes qacE delta 1, sul1, and dfrA5.
Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family.
The study identified multiple AMR genes in E. coli isolates from marine bivalves, including bla TEM-1, bla CTX-M-14, bla CTX-M-15, and various aminoglycoside, trimethoprim, sulfonamide, tetracycline, chloramphenicol, quinolone, and macrolide resistance genes. These findings highlight the potential risk of MDR Enterobacteriaceae in marine environments.
Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic Escherichia coli Sequence Type 95 Strains.
The study identifies several AMR genes in ST95 E. coli strains, including blaTEM-1, blaCTX-M14, aadA1, aadA2, aadA5, aac3, strA, strB, tetA, tetB, tetD, sul1, sul2, dfrA5, dfrA12, dfrA17, mphA, and catA1, which confer resistance to various antibiotics such as ampicillin, cephalothin, streptomycin, tetracycline, sulfamethoxazole, trimethoprim, azithromycin, and chloramphenicol.
Antimicrobial resistance of Klebsiella pneumoniae stool isolates circulating in Kenya.
The study identified 46 AMR genes or gene families in 90 Klebsiella pneumoniae isolates from Kenya, highlighting the prevalence of multidrug resistance and the diversity of resistance mechanisms.
Porcine commensal Escherichia coli: a reservoir for class 1 integrons associated with IS26.
The study identified 17 antimicrobial resistance genes (ARGs) in 103 class 1 integron-positive Escherichia coli strains from porcine feces, highlighting the prevalence of multidrug-resistant commensal E. coli in pigs and their potential role in the dissemination of resistance genes.
Complete Genome Sequence of a bla(CTX-M-1)-Harboring Escherichia coli Isolate Recovered from Cattle in Germany.
The study reports the complete genome sequence of an Escherichia coli isolate carrying the bla(CTX-M-1) gene, along with other resistance genes including mph(A), sul2, dfrA5, strA, and strB, indicating multidrug resistance.
An outbreak of a rare Shiga-toxin-producing Escherichia coli serotype (O117:H7) among men who have sex with men.
The study identified several AMR genes and mutations in STEC O117:H7 isolates, including the azithromycin resistance gene mphA, aadA1, aadA2, aadA5, blaTEM-1B, blaTEM-1C, dfrA1, dfrA12, dfrA14, dfrA, dfrA5, ermB, strA, strB, sul1, sul2, tet(A), tet(B), qnrs1, and a mutation in gyrA (S83L) associated with fluoroquinolone resistance.
A pan-genome-based machine learning approach for predicting antimicrobial resistance activities of the Escherichia coli strains.
The study identifies and characterizes several AMR genes in E. coli, including beta-lactamases (blaTEM-1, blaOXA-1, blaCMY-2, ampC), aminoglycoside modifying enzymes (aac(3')-Ia, aac(3')-VI), dihydrofolate reductases (dfrA1, dfrA5, dfrA12, dfrA15), quinolone resistance proteins (qnrB2, qnrB6, qnrS2), and others. These genes were validated through computational and wet lab methods, showing their roles in conferring resistance to various antibiotics.
Dynamics of antimicrobial resistance in intestinal Escherichia coli from children in community settings in South Asia and sub-Saharan Africa.
The study identified multiple AMR genes in aEPEC isolates from children in South Asia and sub-Saharan Africa, highlighting the prevalence of resistance to multiple antibiotics, including ampicillin, streptomycin, trimethoprim/sulphamethoxazole, and tetracycline.
Emerging Multidrug-Resistant Hybrid Pathotype Shiga Toxin-Producing Escherichia coli O80 and Related Strains of Clonal Complex 165, Europe.
The study identifies multidrug-resistant hybrid pathotype Shiga toxin-producing E. coli O80 strains belonging to clonal complex 165, which harbor a mosaic plasmid with genes conferring resistance to multiple antibiotics, including beta-lactams, tetracyclines, sulfonamides, trimethoprim, kanamycin, streptomycin, and colistin.
Diversity, Virulence, and Antimicrobial Resistance in Isolates From the Newly Emerging Klebsiella pneumoniae ST101 Lineage.
The study characterizes the antimicrobial resistance genes and mutations in the emerging Klebsiella pneumoniae ST101 lineage, highlighting the presence of multiple resistance mechanisms including carbapenemases, extended-spectrum beta-lactamases, and various other resistance genes.
Diverse Commensal Escherichia coli Clones and Plasmids Disseminate Antimicrobial Resistance Genes in Domestic Animals and Children in a Semirural Community in Ecuador.
The study identified various antimicrobial resistance (AMR) genes in commensal Escherichia coli isolates from children and domestic animals in a semirural community in Ecuador. These genes included blaTEM-1B, dfrA8, qnrB19, strA, strB, tetA, tetB, sul1, sul2, and others, contributing to resistance against multiple antibiotics such as ampicillin, trimethoprim, tetracycline, and sulfamethoxazole. The research highlights the role of plasmids in disseminating these AMR genes and emphasizes the complexity of AMR transmission in such environments.
Comparative Genomics and Phenotypic Investigations Into Antibiotic, Heavy Metal, and Disinfectant Susceptibilities of Salmonella enterica Strains Isolated in Australia.
The study identified several AMR genes and mutations in Salmonella enterica strains, including blaTEM-1, aph(3')-I, aph(6')-ld, dfrA5, tetA, and various arsenic resistance genes. A mutation in gyrA was also found to confer nalidixic acid resistance.
The Resistome, Mobilome, Virulome and Phylogenomics of Multidrug-Resistant Escherichia coli Clinical Isolates from Pretoria, South Africa.
The study characterized the resistome, mobilome, and virulome of 20 multidrug-resistant E. coli isolates from Pretoria, South Africa. Key findings include the identification of various beta-lactamase genes (blaCTX-M-15, blaCTX-M-14, blaCTX-M-27, blaOXA-1, blaOXA-10, blaTEM-1B), aminoglycoside resistance genes (aac(3)-IIa, aac(3)-IId, aac(6')-Ib-cr, mph(A)), sulfonamide resistance genes (sul1, sul2, sul3), dihydrofolate reductase genes (dfrA17, dfrA14, dfrA1, dfrA5, dfrA7, dfrA12, dfrA23), tetracycline resistance genes (tet(A), tet(B)), chloramphenicol resistance genes (catB3, catA1), and fluoroquinolone resistance mutations in gyrA, gyrB, parC, and parE.
Use of whole genome sequencing of commensal Escherichia coli in pigs for antimicrobial resistance surveillance, United Kingdom, 2018.
The study analyzed 515 E. coli isolates from pigs using whole genome sequencing to identify AMR genes and mutations. Key findings include the prevalence of blaTEM-1b, tet(A), and tetA(B) genes, along with various mutations in gyrA, parC, and parE that confer resistance to fluoroquinolones. The study highlights the effectiveness of WGS in predicting AMR phenotypes with high concordance to MIC results.
Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China.
The study characterizes an IMP-4-producing Klebsiella pneumoniae ST1873 strain, identifying multiple antimicrobial resistance genes including blaIMP-4, blaSHV-2, blaSHV-11, oqxA, oqxB, aph(6)-Id, strA, catA1, dfrA5, sul2, and fosA.
Comparative analysis of multidrug resistance plasmids and genetic background of CTX-M-producing Escherichia coli recovered from captive wild animals.
The study identifies multiple AMR genes and mutations in MDR E. coli strains from captive wild animals, highlighting the presence of CTX-M-8 and CTX-M-65 beta-lactamases, along with various other resistance mechanisms such as aminoglycoside, tetracycline, and fluoroquinolone resistance genes, as well as mutations in quinolone resistance-determining regions.
Genomic analysis of Escherichia coli strains isolated from diseased chicken in the Czech Republic.
The study identified multiple AMR genes and mutations in E. coli isolates from diseased chickens in the Czech Republic, highlighting the presence of multidrug-resistant strains with resistance to β-lactams, quinolones, sulfonamides, and tetracyclines.
Co-Occurrence of the bla (KPC-2) and Mcr-3.3 Gene in Aeromonas caviae SCAc2001 Isolated from Patients with Diarrheal Disease.
The study identifies multiple antimicrobial resistance genes in Aeromonas caviae SCAc2001, including blaKPC, mcr-3.3, and others, highlighting the potential public health risks posed by this strain.
Comparative Genomic Analysis of 450 Strains of Salmonella enterica Isolated from Diseased Animals.
The study identified 60 antimicrobial resistance genes (ARGs), 4 disinfectant resistance genes (DRGs), and 33 heavy metal resistance genes (HMRGs) in 450 Salmonella strains isolated from diseased animals. These genes contributed to resistance against multiple antimicrobial classes, including aminoglycosides, beta-lactams, tetracyclines, sulfonamides, and phenicols.
Employing MIC Data for Mink Pathogens to Propose Tentative Epidemiological Cut-Off Values: A Step Toward Rationalizing Antimicrobial Use in Mink.
The study identified several AMR genes in mink pathogens, including beta-lactamases (blaTEM-1, blaCTX-M-1), tetracycline resistance genes (tet(A), tet(B)), aminoglycoside resistance genes (aadA5, aadA1), sulfonamide resistance genes (sul2), dihydrofolate reductase genes (dfrA1, dfrA5, dfrA8, dfrA14), macrolide/lincosamide/streptogramin B resistance genes (erm), lincomycin resistance gene (lnu(A)), spectinomycin resistance gene (spc), and additional sulfonamide and trimethoprim resistance genes (sul1, sul3, dfrK, dfrG).
Extended-Spectrum β-Lactamases in Human Isolates of Multidrug-Resistant Non-typhoidal Salmonella enterica.
The study identified several β-lactamase genes, including bla CTX-M-5, bla OXA-1, bla CTX-M-15, bla CTX-M-3, and bla TEM-1, which contribute to extended-spectrum β-lactamase (ESBL) production in multidrug-resistant non-typhoidal Salmonella enterica isolates. Additionally, a mutation in the gyrA gene (D87N) was linked to quinolone resistance.
A Longitudinal Evaluation of the Bacterial Pathogens Colonizing Chronic Non-Healing Wound Sites at a United States Military Treatment Facility in the Pacific Region.
The study identified multiple antimicrobial resistance genes in bacterial isolates from chronic non-healing wounds, including beta-lactamases, aminoglycoside modifying enzymes, macrolide resistance genes, and others. These genes were found in various bacterial species such as E. coli, S. aureus, P. aeruginosa, and others.
Integron activity accelerates the evolution of antibiotic resistance.
The study shows that integron activity accelerates the evolution of antibiotic resistance by enabling cassette re-shuffling and duplication, particularly for aadB, blaVEB-1, and dfrA5 genes, which confer resistance to gentamicin, beta-lactams, and trimethoprim, respectively.
Wild Boars Carry Extended-Spectrum β-Lactamase- and AmpC-Producing Escherichia coli.
Wild boars in Germany carry ESBL/AmpC-producing E. coli, with bla CTX-M-1, bla SHV-12, and bla CMY-2 being the most common beta-lactamase genes. Additional resistance genes include sul1, sul2, sul3, dfrA1, dfrA5, dfrA14, dfrA17, tet(A), cmlA, floR, and qnrS.
Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece.
The study identified several AMR genes in ESBL-producing E. coli isolates from animals in Greece, including bla CTX-M-1/15, bla TEM, aadA1, aadA2, aphA, strA, strB, sul1, sul2, sul3, dfrA1, dfrA5, dfrA7, dfrA12, dfrA14, dfrA15, dfrA17, dfrA19, mph, mrx, intI1, tnpISE cp1, qnrS, and qnrB.
Genomic Insights into Drug Resistance and Virulence Platforms, CRISPR-Cas Systems and Phylogeny of Commensal E. coli from Wildlife.
The study identified various AMR genes in commensal E. coli strains from wildlife, including bla TEM-1, bla CTX-M-1, tet(A), tet(B), and several resistance gene cassettes in integrons. These genes were found to confer resistance to multiple antibiotics, highlighting the presence of AMR in wildlife E. coli populations.
Prevalence and mechanisms of antibiotic resistance in Escherichia coli isolated from mastitic dairy cattle in Canada.
The study identified several AMR genes in E. coli isolates from bovine mastitis, including beta-lactamases (blaTEM-1, blaCARB-3, blaCMY-59), tetracycline resistance genes (tetA, tetB, tetC), aminoglycoside resistance genes (aph(3')-Ia, aph(3'')-Ib, aph(6)-Id, aadA2), and multidrug efflux pump genes (acrA, acrB, acrD, tolC, baeR, emrA, emrB).
CRISPR Element Patterns vs. Pathoadaptability of Clinical Pseudomonas aeruginosa Isolates from a Medical Center in Moscow, Russia.
The study identified multiple antibiotic resistance genes in clinical Pseudomonas aeruginosa isolates, including blaVIM-2, blaOXA-396, blaOXA-488, floR, tetG, sul1, dfrA5, dfrB2, dfrB5, aph(3)-Ib, blaPAO, catB7, and fosA, which confer resistance to various antibiotics such as beta-lactams, chloramphenicol, tetracycline, sulfonamides, trimethoprim, aminoglycosides, and fosfomycin.
Genetic Characterization of Antibiotic Resistant Enterobacteriaceae Isolates From Bovine Animals and the Environment in Nigeria.
The study characterizes antibiotic resistance genes in Enterobacteriaceae isolates from bovine animals and the environment in Nigeria, identifying several beta-lactamase, aminoglycoside modifying enzymes, qnr, sulfonamide, tetracycline, and trimethoprim resistance genes, highlighting the presence of multidrug-resistant strains.
Identifying the Sources of Intestinal Colonization With Extended-Spectrum β-Lactamase-Producing Escherichia coli in Healthy Infants in the Community.
The study identifies several AMR genes, including blaCTX-M-15, qnrS1, ermB, aadA1, aph(3')-Ib, aph(6)-Id, dfrA1, catA1, aadA2, dfrA5, dfrA12, qnrS13, aac(6')-Ib-cr5, blaTEM-1, blaOXA-1, sul1, and dfrA17, associated with ESBL-Ec in children, mothers, and drinking water in rural Bangladesh.
Structure-guided functional studies of plasmid-encoded dihydrofolate reductases reveal a common mechanism of trimethoprim resistance in Gram-negative pathogens.
The study identifies D27E and L28Q substitutions in DfrA1 and DfrA5 as key determinants of trimethoprim resistance, revealing a common mechanism of resistance in plasmid-encoded dihydrofolate reductases.
Antimicrobial resistance in urinary pathogens and culture-independent detection of trimethoprim resistance in urine from patients with urinary tract infection.
The study identifies dfrA1, dfrA5, dfrA7, dfrA12, and dfrA17 as trimethoprim resistance genes in urinary pathogens, demonstrating their utility in predicting phenotypic resistance without culture.
Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece.
The study identified a high prevalence of ESBL-producing Enterobacteriaceae in Greek pigs, with a focus on resistance mechanisms involving bla CTX-M1/15, bla TEM, and bla SHV genes, as well as resistance to fluoroquinolones, aminoglycosides, sulfonamides, trimethoprim, macrolides, and colistin.
High Prevalence of Beta-Lactam-Resistant Escherichia coli in South Australian Grey-Headed Flying Fox Pups (Pteropus poliocephalus).
The study identified various beta-lactam-resistant Escherichia coli strains in South Australian grey-headed flying fox pups, including resistance genes such as blaTEM-1B, blaTEM-1C, blaTEM-33, blaCMY-2, dfrA17, aadA5, dfrA5, sul2, tet(A), tet(B), and catA1.
Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China.
The study identifies tet(X4) as a major cause of tigecycline resistance in E. coli ST761 isolates from a pig farm in China. The gene is located on a hybrid plasmid and is part of a multidrug resistance region that includes other resistance genes such as blaTEM-1, tet(A), tet(M), floR, qnrS1, sul3, dfrA5, and mef(B).
Occurrence of Antimicrobial-Resistant Escherichia coli in Marine Mammals of the North and Baltic Seas: Sentinels for Human Health.
The study identified antimicrobial-resistant Escherichia coli in marine mammals from the North and Baltic Seas, highlighting the presence of resistance genes such as blaTEM, strA, strB, aadA1, sul1, sul2, tet(A), tet(B), tet(D), qnrS, floR, catA1, blaOXA-1-like, blaSHV, and blaCMY-2.
Molluscs-A ticking microbial bomb.
The paper discusses the presence of antibiotic resistance genes (ARGs) in bivalve molluscs, highlighting the spread of resistance to various antibiotics such as colistin, beta-lactams, fluoroquinolones, and tetracyclines. It emphasizes the role of bivalve aquacultures in the dissemination of ARGs and the potential risks to human health through the food chain.
Phylogenetic analysis and antibiotic resistance of Escherichia coli isolated from wild and domestic animals at an agricultural land interface area of Salaphra wildlife sanctuary, Thailand.
The study identified several AMR genes in E. coli isolates from wild and domestic animals in Thailand, including blaTEM, blaSHV, blaCMY-2, aac(3)-IV, aadA, tetA, tetB, qnrA, sul3, dfrA1, dfrA5, and dfrA7, which conferred resistance to various antibiotics such as ampicillin, gentamicin, tetracycline, ciprofloxacin, and trimethoprim-sulfamethoxazole.
Enterobacterales plasmid sharing amongst human bloodstream infections, livestock, wastewater, and waterway niches in Oxfordshire, UK.
The study identifies various AMR genes, including bla TEM-1, aph(3'')-Ib, aph(6)-Id, dfrA5, sul2, and robA, which are associated with plasmids shared among human bloodstream infections, livestock, wastewater, and waterways in Oxfordshire, UK.
Virotyping and genetic antimicrobial susceptibility testing of porcine ETEC/STEC strains and associated plasmid types.
The study identified several AMR genes and mutations in porcine ETEC/STEC strains, including beta-lactamases (blaTEM-1A, blaTEM-1B, blaTEM-106), polymyxin resistance genes (mcr-1.1, mcr-2.1, mcr-5.1), aminoglycoside resistance genes (aac(3)-IId, aac(3)-IV, aac(3)-IVa, aph(3')-Ia, aadA1, aadA10, aadA12), florfenicol resistance gene (floR), tetracycline resistance genes (tet(A), tet(B)), quinolone resistance gene (qnrS1), and trimethoprim-sulfamethoxazole resistance genes (dfrA1, dfrA5, dfrA12, dfrA14, dfrA36).
Molecular epidemiology of enterically colonizing Escherichia coli with resistance against third-generation cephalosporins isolated from stool samples of European soldiers with concomitant diarrhea on deployment in Western African Mali.
The study identified bla CTX-M-15 and bla TEM-1b as the primary genes responsible for third-generation cephalosporin resistance in E. coli isolates from European soldiers in Mali. Additionally, several quinolone resistance genes including qnrS1, gyrA S83L, gyrA D87N, parE S458T, parE S458A, and parC S80I were found. Trimethoprim-sulfamethoxazole resistance was mediated by sul1, sul2, dfrA1, dfrA5, dfrA14, and dfrA17. Gentamicin resistance was associated with aph6-Id, aph3-Ib, aac3-IId, aadA5, and aac6-Ib-cr5. Tetracycline resistance was conferred by tetA, tetB, and tetD.
Dominance of Escherichia coli sequence types ST73, ST95, ST127 and ST131 in Australian urine isolates: a genomic analysis of antimicrobial resistance and virulence linked to F plasmids.
The study identified various antimicrobial resistance genes in E. coli isolates from Australian urine samples, including blaCTX-M-15, blaCTX-M-14, blaTEM-28, sul1, sul2, sul3, dfrA17, dfrA5, dfrA1, dfrB4, tetA, tetB, mphA, cmlA1, cmlA5, catB3, sat2, qnrD1, fosA7, aac(3)-IId, aac(3)-IIe, aph(3')-IIa, aph(6)-Id, ant(3'')-IIa, intI1, and intI2. These genes were associated with resistance to various antibiotics such as beta-lactams, sulfonamides, trimethoprim, tetracycline, macrolides, chloramphenicol, streptothricin, quinolones, fosfomycin, and aminoglycosides.
Antibiotic Resistance Mediated by Escherichia coli in Kuwait Marine Environment as Revealed through Genomic Analysis.
The study identified various antibiotic resistance genes in Escherichia coli isolates from Kuwait's marine environment, including beta-lactamases, aminoglycoside-modifying enzymes, fluoroquinolone resistance genes, sulfonamide resistance genes, tetracycline resistance genes, and macrolide resistance genes. Additionally, the MFS-type drug efflux gene mdfA was commonly found in E. coli isolates.
Polyclonal Multidrug ESBL-Producing Klebsiella pneumoniae and Emergence of Susceptible Hypervirulent Klebsiella pneumoniae ST23 Isolates in Mozambique.
The study identified multiple AMR genes in Klebsiella isolates, including bla CTX-M-15, bla TEM-1, bla SHV, and bla OXA-1, which confer resistance to beta-lactams. Other genes like aac(3)-IIa, aac(6')-Ib-cr, qnrB1, qnrB6, catA1, catA2, catB3, dfrA5, dfrA7, dfrA12, dfrA14, dfrA27, sul1, sul2, mph(A), tet(A), tet(D), fosA, ARR-3, and oqxAB were also found, contributing to resistance against aminoglycosides, quinolones, chloramphenicol, trimethoprim, sulfamethoxazole, macrolides, tetracycline, fosfomycin, rifampicin, and quinolones respectively. Mutations in ompK36, ompK37, and acrR were associated with resistance to cephalosporins and fluoroquinolones, while mutations in RamR were linked to tigecycline resistance.
Comparative Genomic Analysis Reveals the Emergence of ST-231 and ST-395 Klebsiella pneumoniae Strains Associated with the High Transmissibility of bla(KPC) Plasmids.
The study identifies multiple AMR genes, including blaOXA-232, blaCTX-M-15, dfrA14, aac(6')-Ib-cr, and others, in K. pneumoniae isolates, highlighting the role of integrons and plasmids in the dissemination of resistance.
Characterization of integrons, extended spectrum beta lactamases and genetic diversity among uropathogenic Escherichia coli isolates from Kerman, south east of Iran.
The study identified the presence of class I integrons and ESBLs, particularly bla CTX-M, bla TEM, and bla SHV, in uropathogenic E. coli isolates from Kerman, Iran. Additionally, various gene cassettes such as dfrA17-aadA5, aadA1-dfrA1, and others were detected, contributing to resistance against multiple antibiotics.
Occurrence and temporal distribution of extended-spectrum β-lactamase-producing Escherichia coli in clams from the Central Adriatic, Italy.
The study identified several β-lactamase genes, including bla CTX-M-1, bla CTX-M-14, bla CTX-M-15, bla CTX-M-27, bla CTX-M-55, bla SHV12, bla CMY-2, bla TEM-1B, bla TEM-106, and bla TEM-126, which confer resistance to various β-lactam antibiotics. Other resistance genes such as aac(6′)-Ib-cr, qnrS1, tet(A), tet(B), tet(M), aadA5, aadA2b, sul1, sul2, sul3, dfrA17, dfrA5, dfrA14, dfrA12, mph(A), cmlA1, catA2, aac(3)-IIa, aac(3)-IId, and lnu(F) were also characterized, providing insights into the multidrug resistance profiles of ESBL-producing E. coli isolates from clams in the Central Adriatic.
Dynamics of Antimicrobial Resistance Carriage in Koalas (Phascolarctos Cinereus) and Pteropid Bats (Pteropus Poliocephalus) Before, During and After Wildfires.
The study identified various antimicrobial resistance genes, including class 1 integrons and bla TEM genes, in koalas and Pteropid bats before, during, and after wildfires. The presence of these genes was influenced by factors such as fire-impact, captivity, and antibiotic usage.
Emergence of carbapenem resistant gram-negative pathogens with high rate of colistin resistance in Egypt: A cross sectional study to assess resistance trends during the COVID-19 pandemic.
The study identified high prevalence of carbapenem resistance in Gram-negative pathogens in Egypt, with bla NDM and bla OXA-48-like being the most prevalent carbapenemase genes. Plasmid-mediated quinolone resistance genes qnrS and qnrB were also detected. Additionally, several aminoglycoside resistance genes and integron-associated gene cassettes were characterized.
Klebsiella pneumoniae sequence type 147: a high-risk clone increasingly associated with plasmids carrying both resistance and virulence elements.
The study identified various AMR genes and mutations in Klebsiella pneumoniae ST147 isolates, including bla NDM-5, bla NDM-1, bla OXA-181, bla OXA-232, bla OXA-48, aadA1, aph(3')-VI, bla CTX-M-15, bla TEM-1B/C, bla OXA-9, truncated catA1, qnrS1, sul1, dfrA5, mph(A), erm(B), aac(6')-Ib, aac(6')-Ib3, sul2, aph(3')-Ia, rmtB, fosA, oqxAB, bla SHV-11/67, arr-3, and catB3.
Cryptic environmental conjugative plasmid recruits a novel hybrid transposon resulting in a new plasmid with higher dispersion potential.
The study identifies a novel hybrid transposon Tn7714 carrying multiple antibiotic resistance genes (ARGs) that was transferred from an IncF plasmid to a cryptic IncX plasmid, resulting in a new multidrug-resistant conjugative plasmid with significantly higher conjugation efficiency.
Multidrug-Resistant Bacteria in Surgical Intensive Care Units: Antibiotic Susceptibility and β-Lactamase Characterization.
The study identified OXA-48 carbapenemase in 82.9% of K. pneumoniae isolates and NDM in 7.3%. bla CTX-M-15, bla SHV, and bla OXA-1 were also detected. Additionally, aac(6")-Ib, dfrA14, oqxA, and oqxB were found to confer resistance to aminoglycosides, trimethoprim, and fluoroquinolones.
Occurrence, molecular characterization, and antimicrobial susceptibility of sorbitol non-fermenting Escherichia coli in lake water, fish and humans in central Oromia, Ethiopia.
The study identified multiple antimicrobial resistance genes in sorbitol non-fermenting E. coli strains, including blaTEM-1B, tet(A), dfrA1, dfrA5, sul1, fos7, catA1, qnrS1, gyrA, and blaCTX-M15, which conferred resistance to various antibiotics such as ampicillin, tetracycline, trimethoprim, sulfamethoxazole, fosfomycin, chloramphenicol, ciprofloxacin, nalidixic acid, and extended-spectrum beta-lactams.
Occurrence and genomic characterization of antimicrobial-resistant and potential pathogenic Escherichia coli from Italian artisanal food productions of animal origin.
The study identified multiple antimicrobial resistance (AMR) genes in Escherichia coli isolates from Italian artisanal food products, including beta-lactamases, aminoglycoside-modifying enzymes, trimethoprim resistance genes, macrolide resistance genes, quinolone resistance proteins, sulfonamide resistance proteins, and tetracycline resistance genes. These genes were primarily carried on plasmids and contributed to multidrug resistance.
Costs of antibiotic resistance genes depend on host strain and environment and can influence community composition.
The study identifies that certain antibiotic resistance genes (ARGs) such as bla TEM-116 *, cat, and dfrA5 impose fitness costs in various Escherichia spp. strains, highlighting the variability of these effects across different host backgrounds.
Genetics of resistance to trimethoprim in cotrimoxazole resistant uropathogenic Escherichia coli: integrons, transposons, and single gene cassettes.
The study identifies various dfrA gene cassettes, including dfrA1, dfrA5, dfrA7, dfrA8, and dfrA14, as the primary genetic determinants of trimethoprim resistance in cotrimoxazole-resistant uropathogenic E. coli strains. These genes are found in class 1 and 2 integrons, as well as in transposons.
Antibiotic Resistance Genes and Plasmid Characteristics in Enterobacteriaceae Isolated from Fresh Produce
The study identified several antibiotic resistance genes on plasmids and chromosomes of Enterobacteriaceae strains isolated from fresh produce, highlighting the diversity of resistance mechanisms and the potential for horizontal gene transfer.
Molecular Characterization of Multidrug-Resistant Escherichia coli from Fecal Samples of Wild Animals.
The study identified multiple AMR genes in E. coli isolates from wild animals, including beta-lactamases (bla TEM-1B, bla CTX-M-65, bla CTX-M-55, bla EC-1982), aminoglycoside resistance genes (aac(3)-IIa, aadA2, aadA5, ant(3")-Ia, aph(3")-Ib, aph(3′)-Ia, aph(6)-Id), tetracycline resistance genes (tetB, tetA), trimethoprim resistance genes (dfrA17, dfrA1, dfrA5, dfrA12), sulfonamide resistance genes (sul1, sul2, sul3), macrolide/lincosamide/streptogramin resistance genes (mphB, lnuF, ermC, mefC), quinolone resistance genes (qnrB19, qnrB5, qnrS1, qnrS2), and others. Additionally, point mutations in gyrA, parC, and parE were associated with fluoroquinolone resistance.
Emergence of mcr-8.1-bearing MDR-hypervirulent Klebsiella pneumoniae ST307.
The study identifies the colistin resistance gene mcr-8.1 in multidrug-resistant Klebsiella pneumoniae ST307 isolates from Armenia, highlighting the emergence of this gene in the region.
Characterizing Methicillin-Resistant Staphylococcus spp. and Extended-Spectrum Cephalosporin-Resistant Escherichia coli in Cattle.
The study identified several AMR genes in E. coli and MRSA isolates from cattle in Austria, including bla CTX-M-1/15, bla CTX-M-9, bla TEM, tet (A), tet (B), dfrA1, dfrA5, dfrA14, dfrA17, sul2, sul3, aadA1, aadA2, floR, cmlA, aphA, and bla ACT. Additionally, the MRSA isolate carried the mecA gene, indicating methicillin resistance.
Fecal carriage of ESBL-producing E. coli and genetic characterization in rural children and livestock in the Somali region, Ethiopia: a one health approach.
The study identified bla CTX-M-15 as the most prevalent ESBL gene in both human and animal E. coli isolates, along with other resistance genes such as bla TEM-1B, bla OXA-1, and various aminoglycoside, sulfonamide, and trimethoprim resistance genes. Mutations in gyrA, parC, and parE were also associated with fluoroquinolone resistance.
Antibiotic Resistance in Mammalian Wild Game: A Meta-Analysis
The study presents a comprehensive meta-analysis of antibiotic resistance in bacteria isolated from mammalian wild game, highlighting the prevalence of various AMR genes and mutations across different bacterial species.
Multidrug-resistant Shigella flexneri outbreak affecting humans and non-human primates in New Mexico, USA.
The study identifies multidrug-resistant Shigella flexneri serotype 2a strains in both human and non-human primate populations in New Mexico, USA, carrying resistance genes such as blaOXA-1, blaTEM-1, sul2, dfrA1, and dfrA5, along with fluoroquinolone resistance-conferring mutations in parC and gyrA.
Genomic Insights of Antibiotic-Resistant Escherichia coli Isolated from Intensive Pig Farming in South Africa Using 'Farm-to-Fork' Approach.
The study identified multiple antibiotic resistance genes and mutations in E. coli isolates from intensive pig farming in South Africa, highlighting the spread of resistance across the pork production continuum.
Trimethoprim resistance in Escherichia coli exhibits an allele-specific growth advantage.
The study identifies that specific dfrA alleles, such as dfrA1, dfrA5, dfrA7, dfrA14, and dfrA17, confer trimethoprim resistance in E. coli. The growth behavior of isolates carrying these alleles varies, with some showing a faster growth phenotype in the presence of trimethoprim.
Genomic and epidemiological characteristics of Shigella boydii in Australia, 1991-2022.
The study identified high levels of antimicrobial resistance in Shigella boydii, with over 60% of isolates classified as multidrug-resistant. Key resistance genes included blaCTX-M-15, blaCTX-M-3, blaDHA-1, mphA, sul1, sul2, dfrA1, dfrA14, dfrA5, dfrA12, dfrA17, dfrA7, aadA1, aph(3’’)-Ib, aph(6)-Id, tet(A), and tet(B). Mutations in gyrA and parC were associated with ciprofloxacin resistance and reduced susceptibility.
Antibiotic resistance in mastitis-causing bacteria: Exploring antibiotic-resistance genes, underlying mechanisms, and their implications for dairy animal and public health.
The study identifies several AMR genes and mutations in Staphylococcus aureus and coagulase-negative staphylococci associated with mastitis, including blaZ, mecA, tetK, tetM, aphA3, aacA-aphD, aadD, ermA, msrA, mphC, lnuB, and vanA, which confer resistance to various antibiotics such as β-lactams, tetracyclines, aminoglycosides, macrolides, and glycopeptides.
Emerging threat of antimicrobial resistance determinants and plasmid replicon types acquisition by Escherichia coli of poultry and other food-producing animal origin in China: local findings with global implications.
The study identifies multiple antimicrobial resistance genes in Escherichia coli from poultry and other food-producing animals in China, highlighting the prevalence of beta-lactamases, tetracycline resistance genes, aminoglycoside modifying enzymes, quinolone resistance genes, and sulfonamide resistance genes.
Genomic Characterization and Resistance Mechanisms of Carbapenem-Resistant Klebsiella pneumoniae ST101 Isolates from Saudi Arabia.
The study identifies bla_OXA-48 and bla_NDM-1 carbapenemase genes in CRKP ST101 isolates from Saudi Arabia, highlighting their role in carbapenem resistance and the complexity of resistance mechanisms in high-risk clones.
Unveiling Equine Abortion Pathogens: A One Health Perspective on Prevalence and Resistance in Northwest China.
The study identified multiple antibiotic resistance genes in equine abortion pathogens, including CTX-M, TEM-1, TetM, ermA/B/C, qnrA/B, sul1/2, dfrA1/5, SHV, OXA-1, OXA-23/48/58, mecA/B/C, IMP-1/2, NDM-1, VIM-1/2, and vanA/B/C, highlighting the widespread resistance to various antibiotics among these pathogens.
Prevalence of integrons and a new dfrA17 variant in Gram-negative bacilli which cause community-acquired infections., Occurrence of integron-associated resistance gene cassettes located on antibiotic resistance plasmids isolated from a wastewater treatment plant., Site-specific recombination promotes linkage between trimethoprim- and sulfonamide resistance genes. Sequence characterization of dhfrV and sulI and a recombination active locus of Tn21.
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