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ResLit

estDL136

chloramphenicol hydrolase

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

UniProt

Protein entries specific to estDL136

AMR Rules

Browse AMR rules for estDL136

Validation StatusConfirmed2+ DBs

Overview

Mechanism: Enzymatic inactivation
Validation Method: expression in E. coli and resistance testing
Drug Classes: Chloramphenicol, Florfenicol, CHLORAMPHENICOL, FLORFENICOL
Organisms: Escherichia coli, uncultured bacterium pDL136, Novosphingobium aromaticivorans DSM 12444
Alleles: 2
Papers: 3
Databases: Reference Gene CatalogResFinder DatabaseReslit

Allele Variants2

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
estDL136	Reference Gene CatalogReslit	5	Chloramphenicol, Florfenicol +2	Escherichia coli +1	-	2012, 2019, 2022	JN242251	ABP64512.1

Papers3

Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase

PMID 22752166 · 2012

The study identifies a novel chloramphenicol hydrolase, estDL136, which inactivates chloramphenicol and florfenicol, providing cross-resistance in E. coli.

Gene Information

Gene Name: estDL136
Allele: estDL136
Encodes: chloramphenicol hydrolase
Source Database: Reslit
Confers Resistance To: ChloramphenicolFlorfenicol
Organisms Tested In: • Escherichia coli
Validation Method: expression in E. coli and resistance testing
Sequence Accession: JN242251

Inactivation of chloramphenicol and florfenicol by a novel chloramphenicol hydrolase.

PMID 22752166 · 2012

Gene Information

Gene Name: estDL136
Allele: EstDL136
Source Database: ResFinder Database
Mechanism: Enzymatic inactivation
Confers Resistance To: CHLORAMPHENICOLFLORFENICOL
Organisms Tested In: • uncultured bacterium pDL136
Sequence Accession: JN242251
Notes: Phenicol hydrolase

Crystal structure of chloramphenicol-metabolizing enzyme EstDL136 from a metagenome.

PMID 30645605 · 2019

The study characterizes EstDL136, a metagenome-derived chloramphenicol-metabolizing enzyme, demonstrating its ability to hydrolyze chloramphenicol and confer resistance to bacteria expressing the gene.

Gene Information

Gene Name: estDL136
Allele: estDL136
Encodes: chloramphenicol-metabolizing enzyme
Source Database: Reslit
Mechanism: hydrolysis of chloramphenicol
Confers Resistance To: Chloramphenicol
Organisms Tested In: • Escherichia coli
Validation Method: structural and functional analyses
Sequence Accession: HQ148666

Strategies for Enzymatic Inactivation of the Veterinary Antibiotic Florfenicol.

PMID 35453195 · 2022

The study characterizes the hydrolase EstDL136 and its optimized mutant 36P5, which effectively inactivate florfenicol, demonstrating improved activity and stability in various environments.

Gene Information

Gene Name: estDL136
Allele: estDL136
Encodes: hydrolase
Source Database: Reslit
Confers Resistance To: Florfenicol
Organisms Tested In: • Escherichia coli
Validation Method: growth assays and NMR spectroscopy

Strategies for Enzymatic Inactivation of the Veterinary Antibiotic Florfenicol.

PMID 35453195 · 2022

The study characterizes the hydrolase EstDL136 and its optimized mutant 36P5, which effectively inactivate florfenicol, demonstrating improved activity and stability in various environments.

Gene Information

Gene Name: estDL136
Allele: estDL136
Encodes: hydrolase
Source Database: Reslit
Confers Resistance To: Florfenicol
Organisms Tested In: • Escherichia coli
Validation Method: enzyme activity and bacterial growth assays

PMID N/A

Gene Information

Gene Name: estDL136
Allele: estDL136
Encodes: chloramphenicol hydrolase
Source Database: Reference Gene Catalog
Confers Resistance To: CHLORAMPHENICOLFLORFENICOL
Organisms Tested In: • Novosphingobium aromaticivorans DSM 12444
Sequence Accession: CP000677.1
Protein Accession: ABP64512.1