The study identified multiple antibiotic resistance genes in vancomycin-resistant enterococci (VRE) isolates from a cattle feedlot, including vanC1, vanC2/C3, vanXY-C, VanR, macA, macB, rlmA (II), erm(A), aac(6')-la, blaEC, tet(A), tet(L), S10p, gyrA, gyrB, msbA, S12p, rpoB, mdfA/cmr, liaF, liaR, liaS, bcrC, mprF, pgsA, ef-G, ef-TU, ddl, alr, kasA, isotRNA, inhA, fabl, murA, folA, and Dfr, which confer resistance to various antibiotics such as vancomycin, macrolides, aminoglycosides, β-lactams, tetracyclines, quinolones, and others.