gapA

Glyceraldehyde-3-phosphate dehydrogenase

Validation StatusCandidate

Overview

Mechanism: Inactivation of gapA leads to reduced enzymatic activity, contributing to fosfomycin resistance.
Validation Method: Complementation experiments showed that introducing the wild-type gapA gene restored susceptibility to fosfomycin.
Drug Classes: Fosfomycin
Organisms: Stenotrophomonas maltophilia, Escherichia coli
Geographic Location: Krasnodar Krai
Papers: 2
Databases: Reslit

Papers2

The Inactivation of Enzymes Belonging to the Central Carbon Metabolism Is a Novel Mechanism of Developing Antibiotic Resistance.

PMID 32487742 · 2020

The study identifies mutations in genes encoding enzymes of the Embden-Meyerhof-Parnas (EMP) metabolic pathway as a novel mechanism of fosfomycin resistance in Stenotrophomonas maltophilia.

Gene Information

Gene Name: gapA
Allele: gapA
Encodes: Glyceraldehyde-3-phosphate dehydrogenase
Source Database: Reslit
Mechanism: Inactivation of gapA leads to reduced enzymatic activity, contributing to fosfomycin resistance.
Confers Resistance To: Fosfomycin
Organisms Tested In: • Stenotrophomonas maltophilia
Validation Method: Complementation experiments showed that introducing the wild-type gapA gene restored susceptibility to fosfomycin.
Sequence Accession: NCBI:CP041005|SRX8197527|SRX8197528|SRX8197529|SRX8197530|GSE141276

Novel Selectable Marker Sesquiterpenoid Antibiotic Pentalenolactone.

PMID 39769093 · 2024

The study identified a mutation in the gapA gene of E. coli that confers resistance to pentalenolactone, a sesquiterpenoid antibiotic. This mutation, Thr175Ser, was experimentally validated and can serve as a selectable marker for genetic engineering applications.

Gene Information

Gene Name: gapA
Allele: gapA
Encodes: glyceraldehyde-3-phosphate dehydrogenase
Source Database: Reslit
Mechanism: resistance to pentalenolactone
Organisms Tested In: • Escherichia coli
Geographic Location: Krasnodar Krai
Validation Method: genomic DNA sequencing and Sanger sequencing
Sequence Accession: CP108521.1

Comments (0)

No comments yet. Be the first to comment!

Browse AMR Genes

gapA

Gene Information

Gene Information