leuS

leucine-tRNA synthetase

Validation StatusSupported

Overview

Mechanism: mutant confers resistance to novobiocin via stringent response
Validation Method: mutant isolation and resistance testing
Drug Classes: Novobiocin, Levofloxacin, Ciprofloxacin
Organisms: Escherichia coli K-12, Mycobacterium abscessus, Staphylococcus aureus
Geographic Location: Europe
Papers: 5
Databases: Reslit
Mutations: 1

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
R422C	-	-	Staphylococcus aureus	Ciprofloxacin	Reslit	Candidate

Papers5

tRNA Synthetase Mutants of Escherichia coli K-12 Are Resistant to the Gyrase Inhibitor Novobiocin.

PMID 10217798 · 1999

Mutations in tRNA synthetase genes (alaS, argS, ileS, leuS) and rpoN confer resistance to the gyrase inhibitor novobiocin in Escherichia coli K-12 through the RelA-mediated stringent response.

Gene Information

Gene Name: leuS
Allele: leuS
Encodes: leucine-tRNA synthetase
Source Database: Reslit
Mechanism: mutant confers resistance to novobiocin via stringent response
Confers Resistance To: Novobiocin
Organisms Tested In: • Escherichia coli K-12
Validation Method: mutant isolation and resistance testing

Efficacy of epetraborole against Mycobacterium abscessus is increased with norvaline.

PMID 34637487 · 2021

The study identifies leuS as the target of epetraborole (EPT) in Mycobacterium abscessus, with a D436H mutation in leuS conferring high-level resistance to EPT.

Gene Information

Gene Name: leuS
Allele: leuS
Encodes: leucyl-tRNA synthetase
Source Database: Reslit
Organisms Tested In: • Mycobacterium abscessus
Validation Method: cloning and complementation experiments
Sequence Accession: P9WFV1.1|11896261|6224275|WP005112800.1|WP_003900794.1

DS86760016, a Leucyl-tRNA Synthetase Inhibitor, Is Active against Mycobacterium abscessus.

PMID 37212672 · 2023

Gene Information

Gene Name: leuS
Allele: leuS
Encodes: leucyl-tRNA synthetase
Source Database: Reslit
Organisms Tested In: • Mycobacterium abscessus
Validation Method: sequencing of resistant mutants

A Study of Antibiotic Tolerance to Levofloxacin and Rifampin in Staphylococcus aureus Isolates Causing Prosthetic Joint Infections: Clinical Relevance and Treatment Challenges.

PMID 41594048 · 2025

The study identified sspA and leuS as genes associated with increased levofloxacin tolerance in biofilm-forming S. aureus isolates, and prs and pgm as genes linked to rifampin tolerance in non-biofilm isolates.

Gene Information

Gene Name: leuS
Allele: leuS
Encodes: leucyl-tRNA synthetase
Source Database: Reslit
Confers Resistance To: Levofloxacin
Organisms Tested In: • Staphylococcus aureus
Geographic Location: Europe
Validation Method: genomic analysis and TDtest
Sequence Accession: JAJHMK000000000|JAJHNB000000000|JAJHMI000000000|JAJHNN000000000|JAJHMB000000000|JAJHLP000000000|JAJHLR000000000|JAJHLS000000000|JAJHLQ000000000|JAJHMV000000000|JAJHNG000000000|JAJHNF000000000

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leuS

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