mef(En2)
macrolide efflux pump
Overview
- Drug Classes
- macrolides, erythromycin
- Organisms
- Streptococcus pneumoniae, Bacteroides thetaiotaomicron
- Alleles
- 3
- Papers
- 9
| Allele | Papers | Drug Classes | Organisms | Countries | Years | Status |
|---|---|---|---|---|---|---|
| mefE | 3 | macrolides | Streptococcus pneumoniae | Alabama|South Carolina|Tennessee, Europe|North America|Asia | 2000, 2005, 2023 | Not Validated |
| mefE N2 | 1 | erythromycin | Bacteroides thetaiotaomicron | - | 2000 | Not Validated |
| mef(E) | 5 | erythromycin, macrolides | Streptococcus pneumoniae | Italy, North America|Europe|Africa|Asia, Canada | 2003, 2004, 2005 | Not Validated |
Use of clindamycin disks To detect macrolide resistance mediated by ermB and mefE in Streptococcus pneumoniae isolates from adults and children.
PMID 10790089 · 2000The study identified ermB and mefE as major mechanisms of macrolide resistance in Streptococcus pneumoniae, with ermB causing high-level resistance to clindamycin and macrolides, while mefE conferred lower-level resistance to macrolides but susceptibility to clindamycin.
Gene Information
- Gene Name
- mef(En2)
- Allele
- mefE
- Encodes
- macrolide efflux pump
- Validation Method
- PCR and MIC testing
Mechanism
efflux
Confers Resistance To
Organisms Tested In
Isolation Details
Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene.
PMID 10852890 · 2000The study identifies linA N2 and mefE N2 as functional lincomycin and macrolide resistance genes carried by the Bacteroides mobilizable transposon NBU2.
Gene Information
- Gene Name
- mef(En2)
- Allele
- mefE N2
- Encodes
- macrolide resistance protein
- Validation Method
- expression in Bacteroides thetaiotaomicron and MIC testing
Confers Resistance To
Organisms Tested In
Antimicrobial Resistance Mechanisms and Molecular Detection Techniques
PMID 11585788 · 2023The paper discusses molecular detection methods for antimicrobial resistance, focusing on genes like mecA and blaZ, which confer resistance to beta-lactam antibiotics in staphylococci.
Gene Information
- Gene Name
- mef(En2)
- Allele
- mefE
- Encodes
- macrolide efflux pump
- Validation Method
- PCR and sequencing
Confers Resistance To
Organisms Tested In
Isolation Details
Phenotypic and molecular characterization of tetracycline- and erythromycin-resistant strains of Streptococcus pneumoniae.
PMID 12821474 · 2003The study identified tet(M), erm(B), mef(A), and mef(E) as the primary genes responsible for tetracycline and erythromycin resistance in Streptococcus pneumoniae strains.
Gene Information
- Gene Name
- mef(En2)
- Allele
- mef(E)
- Encodes
- macrolide efflux pump
- Validation Method
- PCR and MIC testing
Confers Resistance To
Organisms Tested In
Isolation Details
Tn2009, a Tn916-like element containing mef(E) in Streptococcus pneumoniae.
PMID 15155196 · 2004The study identifies Tn2009, a composite transposon containing the macrolide efflux gene mef(E) and the tetracycline resistance gene tet(M) in Streptococcus pneumoniae.
Gene Information
- Gene Name
- mef(En2)
- Allele
- mef(E)
- Encodes
- macrolide efflux pump
- Validation Method
- PCR and sequencing
Confers Resistance To
Organisms Tested In
Characterization and prevalence of MefA, MefE, and the associated msr(D) gene in Streptococcus pneumoniae clinical isolates.
PMID 15297499 · 2004The study characterizes the MefA, MefE, and msr(D) genes in Streptococcus pneumoniae clinical isolates, demonstrating that these genes confer resistance to macrolides and ketolides. The msr(D) gene was shown to function independently as an efflux pump.
Gene Information
- Gene Name
- mef(En2)
- Allele
- mef(E)
- Encodes
- efflux pump
- Validation Method
- transformation into a susceptible strain and MIC testing
Confers Resistance To
Organisms Tested In
Isolation Details
Pharmacodynamic activity of telithromycin at simulated clinically achievable free-drug concentrations in serum and epithelial lining fluid against efflux (mefE)-producing macrolide-resistant Streptococcus pneumoniae for which telithromycin MICs vary.
PMID 15855517 · 2005The study characterizes the mefE gene, which encodes an efflux pump responsible for macrolide resistance in Streptococcus pneumoniae. Telithromycin was effective against mefE-producing strains with MICs up to 0.25 μg/ml in serum and 1 μg/ml in epithelial lining fluid.
Gene Information
- Gene Name
- mef(En2)
- Allele
- mefE
- Encodes
- efflux pump
- Validation Method
- PCR and MIC testing
Confers Resistance To
Organisms Tested In
Expression of the mef(E) Gene Encoding the Macrolide Efflux Pump Protein Increases in Streptococcus pneumoniae with Increasing Resistance to Macrolides.
PMID 16251306 · 2005The study shows that increased expression of the mef(E) gene is associated with higher levels of macrolide resistance in Streptococcus pneumoniae.
Gene Information
- Gene Name
- mef(En2)
- Allele
- mef(E)
- Encodes
- macrolide efflux pump protein
- Validation Method
- expression analysis and MIC testing
Confers Resistance To
Organisms Tested In
Isolation Details
Molecular characterization of pneumococci with efflux-mediated erythromycin resistance and identification of a novel mef gene subclass, mef(I).
PMID 16304164 · 2005The study identified a novel mef gene subclass, mef(I), in pneumococci with efflux-mediated erythromycin resistance. It also characterized the presence of tet(M) and tet(O) genes contributing to tetracycline resistance in certain isolates.
Gene Information
- Gene Name
- mef(En2)
- Allele
- mef(E)
- Encodes
- efflux pump
- Validation Method
- PCR-RFLP analysis and sequencing
Confers Resistance To
Organisms Tested In
Isolation Details
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