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mexE

multidrug efflux RND transporter periplasmic adaptor subunit MexE

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

Protein entries specific to mexE

Browse AMR rules for mexE

Validation StatusConfirmed2+ DBs

Overview

Mechanism: antibiotic efflux
Validation Method: transcriptome profiling, promoter-lacZ fusion assays
Drug Classes: EFFLUX, Tetracycline, Ofloxacin, Ciprofloxacin, Chloramphenicol, Trimethoprim, Fluoroquinolone, Bacitracin, Erythromycin, Roxithromycin, Glycylcyclines, Aminoglycoside, Ciprofloxacin|Levofloxacin, Piperacillin/tazobactam|Ceftazidime|Cefepime|Meropenem|Imipenem|Tobramycin|Ciprofloxacin, Fluoroquinolones
Organisms: Pseudomonas aeruginosa PAO1, Pseudomonas aeruginosa, Pseudomonas entomophila L48
Geographic Location: Romania
Alleles: 2
Papers: 11
Databases: Card DatabaseReference Gene CatalogReslit
Mutations: 3

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
S8F	-	-	Pseudomonas aeruginosa	Ciprofloxacin\|Levofloxacin	Reslit	Candidate
G195D	-	-	Pseudomonas aeruginosa	Piperacillin/tazobactam\|Ceftazidime\|Cefepime\|Meropenem\|Imipenem\|Tobramycin\|Ciprofloxacin	Reslit	Candidate
-	T4C	-	Pseudomonas aeruginosa

Allele Variants2

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
MexE	Card DatabaseReference Gene CatalogReslit	9	EFFLUX, Tetracycline +9	Pseudomonas aeruginosa PAO1 +2	-	1999, 2009, 2011, 2012, 2013, 2022, 2024	AE004091.2	AAG05881.1
mex E	Reslit	1	Fluoroquinolone, Aminoglycoside +1	Pseudomonas aeruginosa	Romania	2023	-	-

Papers11

1

Characterization of MexT, the regulator of the MexE-MexF-OprN multidrug efflux system of Pseudomonas aeruginosa.

PMID 10515918 · 1999

Gene Information

Gene Name: mexE
Allele: MexE
Encodes: multidrug efflux RND transporter periplasmic adaptor subunit MexE
Source Database: Reference Gene Catalog
Mechanism: antibiotic efflux
Confers Resistance To: EFFLUX
Organisms Tested In: • Pseudomonas aeruginosa PAO1
Sequence Accession: AE004091.2
Protein Accession: AAG05881.1
Notes: ARO:3000803

2

Characterization of MexT, the regulator of the MexE-MexF-OprN multidrug efflux system of Pseudomonas aeruginosa.

PMID 10515918 · 1999

Gene Information

Gene Name: mexE
Allele: MexE
Encodes: multidrug efflux RND transporter periplasmic adaptor subunit MexE, resistance-nodulation-cell division (RND) antibiotic efflux pump
Source Database: Card Database
Mechanism: antibiotic efflux
Confers Resistance To: EFFLUX
Organisms Tested In: • Pseudomonas aeruginosa PAO1
Sequence Accession: AE004091.2
Protein Accession: AAG05881.1
Notes: ARO:3000803

3

Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa.

PMID 19846594 · 2009

The study identifies a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa, including genes such as mexT, mexE, mexF, oprN, and mexS. The conserved DNA motif required for MexT-mediated gene activation was identified, and the binding of MexT to this motif was confirmed through EMSA.

Gene Information

Gene Name: mexE
Allele: MexE
Encodes: RND multidrug efflux membrane fusion protein
Source Database: Reslit
Mechanism: part of the MexEF-OprN efflux system, which is activated by MexT
Organisms Tested In: • Pseudomonas aeruginosa PAO1
Validation Method: transcriptome profiling, promoter-lacZ fusion assays

4

MexEF-OprN efflux pump exports the Pseudomonas quinolone signal (PQS) precursor HHQ (4-hydroxy-2-heptylquinoline).

PMID 21957445 · 2011

The study identifies the MexEF-OprN efflux pump as a key player in exporting the Pseudomonas quinolone signal (PQS) precursor HHQ, affecting quorum sensing and virulence in Pseudomonas aeruginosa.

Gene Information

Gene Name: mexE
Allele: MexE
Encodes: efflux pump component
Source Database: Reslit
Mechanism: constitutive expression leads to increased resistance
Confers Resistance To: Tetracycline
Organisms Tested In: • Pseudomonas aeruginosa
Validation Method: qRT-PCR and mutant construction

5

The regulatory repertoire of Pseudomonas aeruginosa AmpC ß-lactamase regulator AmpR includes virulence genes.

PMID 22479525 · 2012

AmpR is a global regulator in P. aeruginosa that influences the expression of over 500 genes, including those involved in β-lactam resistance, virulence, and biofilm formation.

Gene Information

Gene Name: mexE
Allele: MexE
Encodes: efflux pump component
Source Database: Reslit
Confers Resistance To: OfloxacinCiprofloxacinChloramphenicolTrimethoprim
Organisms Tested In: • Pseudomonas aeruginosa
Validation Method: microarray and qPCR
Sequence Accession: NCBI:NC_002516

6

Transcriptome profiling reveals links between ParS/ParR, MexEF-OprN, and quorum sensing in the regulation of adaptation and virulence in Pseudomonas aeruginosa.

PMID 24034668 · 2013

The study identifies that the ParS/ParR system regulates the mexEF-oprN operon through mexS, which is involved in resistance to fluoroquinolones, trimethoprim, and chloramphenicol.

Gene Information

Gene Name: mexE
Allele: MexE
Encodes: Multidrug efflux membrane fusion protein
Source Database: Reslit
Confers Resistance To: FluoroquinoloneTrimethoprimChloramphenicol
Organisms Tested In: • Pseudomonas aeruginosa PAO1
Validation Method: RNA-seq and qRT-PCR

7

Antibiotic resistance in plant growth promoting bacteria: A comprehensive review and future perspectives to mitigate potential gene invasion risks.

PMID 36204627 · 2022

This review highlights the prevalence of antibiotic resistance genes (ARGs) in plant growth-promoting bacteria (PGPB) and emphasizes the potential risks of ARG dissemination through biofertilizers. Key findings include the identification of various ARGs such as bacA, fosB, ermD, bl2a, vanSA, cat, acrD, mexF, mexD, mexW, mexE, mexY, mexX, mexZ, mexR, mexA, mexB, mexC, mexD, mexE, and mexF, which confer resistance to multiple antibiotics.

Gene Information

Gene Name: mexE
Allele: MexE
Encodes: efflux pump component
Source Database: Reslit
Confers Resistance To: ChloramphenicolBacitracinErythromycinFluoroquinoloneRoxithromycinGlycylcyclines
Organisms Tested In: • Pseudomonas aeruginosa
Validation Method: Table 1

8

Uncovering the Resistance Mechanisms in Extended-Drug-Resistant Pseudomonas aeruginosa Clinical Isolates: Insights from Gene Expression and Phenotypic Tests.

PMID 37764055 · 2023

The study identified bla GES-2, bla OXA48-like, bla NDM, bla SPM, and bla VIM as the main carbapenemase genes in MDR P. aeruginosa isolates. Overexpression of mex efflux pumps, particularly mex C, was strongly associated with multidrug resistance.

Gene Information

Gene Name: mexE
Allele: mex E
Encodes: efflux pump
Source Database: Reslit
Confers Resistance To: FluoroquinoloneAminoglycosideTetracycline
Organisms Tested In: • Pseudomonas aeruginosa
Geographic Location: Romania
Validation Method: real-time RT-PCR

9

From Proteome to Potential Drugs: Integration of Subtractive Proteomics and Ensemble Docking for Drug Repurposing against Pseudomonas aeruginosa RND Superfamily Proteins.

PMID 39125594 · 2024

The study identifies and characterizes essential proteins in Pseudomonas aeruginosa, particularly RND efflux pumps, which are critical for antibiotic resistance. Computational methods and ensemble docking were used to find potential inhibitors like MK-3207, R-428, and Suramin, showing promise for drug repurposing.

Gene Information

Gene Name: mexE
Allele: MexE
Encodes: membrane fusion protein
Source Database: Reslit
Mechanism: efflux pump
Organisms Tested In: • Pseudomonas aeruginosa
Validation Method: ensemble docking and non-covalent interaction index

10

Gene Information

Gene Name: mexE
Allele: MexE
Encodes: multidrug efflux RND transporter periplasmic adaptor subunit MexE
Source Database: Reference Gene Catalog
Confers Resistance To: EFFLUX
Organisms Tested In: • Pseudomonas entomophila L48
Sequence Accession: CT573326.1
Protein Accession: CAK15125.1