mspA

porin

Validation StatusCandidate

Overview

Validation Method: transposon mutagenesis and bactericidal assays
Drug Classes: Ethidium bromide, IPM|Cefoxitin
Organisms: Mycobacterium smegmatis, Mycobacterium tuberculosis
Papers: 3
Databases: Reslit
Mutations: 1

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
P67L	C200T	-	-	IPM\|Cefoxitin	Reslit	Candidate

Papers3

Decreased outer membrane permeability protects mycobacteria from killing by ubiquitin-derived peptides.

PMID 19682257 · 2009

The study identified mspA as a gene that, when mutated, decreases outer membrane permeability and confers resistance to ubiquitin-derived peptides and other antimicrobial peptides in mycobacteria.

Gene Information

Gene Name: mspA
Allele: mspA
Encodes: porin
Source Database: Reslit
Organisms Tested In: • Mycobacterium smegmatis
• Mycobacterium tuberculosis
Validation Method: transposon mutagenesis and bactericidal assays

Ethidium bromide transport across Mycobacterium smegmatis cell-wall: correlation with antibiotic resistance.

PMID 21332993 · 2011

The study identifies lfrA as a primary efflux pump involved in ethidium bromide extrusion in Mycobacterium smegmatis, and mspA as a major porin affecting ethidium bromide influx. Disruption of lfrA increases susceptibility to several antibiotics, while deletion of mspA reduces ethidium bromide accumulation and enhances resistance.

Gene Information

Gene Name: mspA
Allele: mspA
Encodes: porin
Source Database: Reslit
Confers Resistance To: Ethidium bromide
Organisms Tested In: • Mycobacterium smegmatis
Validation Method: efflux assays and MIC testing

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mspA

Mutations

Gene Information

Gene Information