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ompT

outer membrane protease

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

Protein entries specific to ompT

Browse AMR rules for ompT

Validation StatusConfirmed2+ DBs

Overview

Mechanism: degradation of α-helical antimicrobial peptides
Validation Method: generation of ompT deletion mutants and complementation studies
Drug Classes: Polymyxin b, Aminoglycoside, Polymyxin
Organisms: Escherichia coli, Citrobacter rodentium, Vibrio cholerae O1 El Tor A1552, E. coli, Vibrio cholerae, Enterobacter cloacae
Geographic Location: Canada|USA, China|USA, Northern Palestinian territories|Northern Palestine
Alleles: 3
Papers: 10
Databases: Card DatabaseReslit

Allele Variants3

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
ompT	Reslit	9	Polymyxin b, Aminoglycoside +1	Escherichia coli +4	Canada\|USA, Northern Palestinian territories\|Northern Palestine, China\|USA	2012, 2013, 2019, 2020, 2022, 2023, 2024, 2025	PRJNA551561\|PRJNA551565\|PRJNA551566	-

Papers10

1

OmpT outer membrane proteases of enterohemorrhagic and enteropathogenic Escherichia coli contribute differently to the degradation of human LL-37.

PMID 22144482 · 2012

OmpT outer membrane protease of EHEC and EPEC contributes to the degradation of human LL-37 and synthetic α-helical peptide C18G, with EHEC OmpT showing greater efficiency in degradation compared to EPEC OmpT.

Gene Information

Gene Name: ompT
Allele: ompT
Encodes: outer membrane protease
Source Database: Reslit
Mechanism: degradation of α-helical antimicrobial peptides
Organisms Tested In: • Escherichia coli
• Citrobacter rodentium
Validation Method: generation of ompT deletion mutants and complementation studies

2

Role of the Vibrio cholerae matrix protein Bap1 in cross-resistance to antimicrobial peptides.

PMID 24098113 · 2013

The study identifies Bap1 and OmpT as critical factors in Vibrio cholerae's cross-resistance to antimicrobial peptides Polymyxin B and LL-37. Bap1 binds to OmpT on outer membrane vesicles (OMVs) and traps LL-37, reducing its effectiveness. OmpT's LDV domain is essential for Bap1 binding.

Gene Information

Gene Name: ompT
Allele: ompT
Encodes: major outer membrane porin
Source Database: Reslit
Confers Resistance To: Polymyxin b
Organisms Tested In: • Vibrio cholerae O1 El Tor A1552
Validation Method: Western blot analysis and mutagenesis experiments

3

Antimicrobial Peptides and Their Mechanisms of Action Against Pathogens

Gene Information

Gene Name: ompT
Allele: OmpT
Encodes: outer membrane protease
Source Database: Reslit
Mechanism: proteolysis
Organisms Tested In: • E. coli
Validation Method: OmpT plays an important role in the resistance of E. coli to protamine and urinary cationic peptides

4

Identification and characterization of OmpT-like proteases in uropathogenic Escherichia coli clinical isolates.

PMID 31496120 · 2019

The study identified and characterized OmpT and arlC, two OmpT-like proteases in uropathogenic E. coli (UPEC) clinical isolates, which cleave and inactivate antimicrobial peptides, contributing to antimicrobial resistance.

Gene Information

Gene Name: ompT
Allele: ompT
Encodes: protease
Source Database: Reslit
Mechanism: cleaves and inactivates antimicrobial peptides
Organisms Tested In: • Escherichia coli
Geographic Location: Canada|USA
Validation Method: FRET activity assay, proteolytic cleavage of AMPs
Sequence Accession: PRJNA551561|PRJNA551565|PRJNA551566

5

Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae.

PMID 31901519 · 2020

The study shows that hypervesiculation in Vibrio cholerae leads to faster adaptation to antimicrobial peptides and bile through the depletion of OmpT and accumulation of (di)glycine-modified lipid A, respectively.

Gene Information

Gene Name: ompT
Allele: ompT
Encodes: outer membrane porin
Source Database: Reslit
Mechanism: depletion of OmpT from the OM promotes resistance to bile
Organisms Tested In: • Vibrio cholerae
Validation Method: immunoblot analysis

6

Catestatin selects for colonization of antimicrobial-resistant gut bacterial communities.

PMID 35440728 · 2022

Catestatin treatment promotes the abundance of bacteria harboring antimicrobial resistance genes eptA and ompT, which confer resistance to Catestatin through phosphoethanolamine modification and protease-mediated degradation, respectively.

Gene Information

Gene Name: ompT
Allele: ompT
Encodes: outer membrane protease
Source Database: Reslit
Mechanism: protease-mediated degradation
Organisms Tested In: • Escherichia coli
Validation Method: cloning in E. coli + growth inhibition assay
Sequence Accession: PRJNA741992

7

Omptin Proteases of Enterobacterales Show Conserved Regulation by the PhoPQ Two-Component System but Exhibit Divergent Protection from Antimicrobial Host Peptides and Complement.

PMID 36533918 · 2023

The study identifies that omptin proteases in Enterobacterales, including OmpT, OmpP, ArlC, and CroP, are regulated by the PhoPQ two-component system and provide varying levels of protection against antimicrobial host peptides and complement.

Gene Information

Gene Name: ompT
Allele: ompT
Encodes: protease
Source Database: Reslit
Organisms Tested In: • Escherichia coli
• Citrobacter rodentium
Validation Method: FRET-based activity assay, Western blotting, and susceptibility testing

8

Multiple resistance factors collectively promote inoculum-dependent dynamic survival during antimicrobial peptide exposure in Enterobacter cloacae.

PMID 39186812 · 2024

The study identifies PhoPQ, RcsB, and OmpT as critical factors contributing to cecropin B resistance in Enterobacter cloacae, highlighting their roles in outer membrane modifications, membrane stress response, and proteolytic cleavage, respectively.

Gene Information

Gene Name: ompT
Allele: ompT
Encodes: outer membrane protease
Source Database: Reslit
Mechanism: proteolytic cleavage of cecropin B
Organisms Tested In: • Enterobacter cloacae
Validation Method: deletion mutant and overexpression experiments

9

Molecular characterization of avian pathogenic Escherichia coli isolates from broiler farms in Northern Palestinian territories.

PMID 39897376 · 2024

The study identified several virulence genes, including iroN, hlyF, iutA, tsh, iss, ompT, papGI, papGII, papC, and cva/cvi, which were associated with antibiotic resistance in APEC isolates from broiler farms in Northern Palestine. These genes were found to be prevalent among the isolates and correlated with resistance to multiple antibiotics.

Gene Information

Gene Name: ompT
Allele: ompT
Encodes: outer membrane protease gene (protectins)
Source Database: Reslit
Confers Resistance To: AminoglycosidePolymyxin
Organisms Tested In: • Escherichia coli
Geographic Location: Northern Palestinian territories|Northern Palestine
Validation Method: multiplex PCR

10

Convergence of plasmid-driven virulence and antibiotic resistance in Escherichia coli.

PMID 41372146 · 2025

The study identifies mcr-1 as a key gene conferring colistin resistance in ColVLP co-integrates, highlighting the convergence of virulence and resistance on plasmids in E. coli.

Gene Information

Gene Name: ompT
Allele: ompT
Encodes: outer membrane protease
Source Database: Reslit
Organisms Tested In: • Escherichia coli
Geographic Location: China|USA
Validation Method: expression in E. coli and functional assays

11

Gene Information

Gene Name: ompT
Allele: Vcho_OmpT
Encodes: General Bacterial Porin with reduced permeability to peptide antibiotics
Source Database: Card Database
Mechanism: reduced permeability to antibiotic
Organisms Tested In: • Vibrio cholerae
Sequence Accession: AF079766.2
Protein Accession: AAC28105.1
Notes: ARO:3004118