pac

puromycin-N-acetyl transferase

Validation StatusSupported

Overview

Mechanism: confers resistance to puromycin by enzymatic modification
Validation Method: functional testing in E. coli and HeLa cells
Drug Classes: Puromycin
Organisms: Escherichia coli, Mycoplasma mycoides subsp. mycoides, Mycoplasma capricolum subsp. capricolum, Mycoplasma genitalium, Mycoplasma gallisepticum, Mycoplasma pneumoniae, Streptomyces sp. VKM Ac-502, E. coli
Papers: 3
Databases: Reslit

Papers3

A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination.

PMID 19105832 · 2008

The study presents a set of vectors (pINS) containing antibiotic resistance genes for puromycin, blasticidin S, and G418, enabling efficient Cre-mediated recombination for introducing these resistance markers into target vectors.

Gene Information

Gene Name: pac
Allele: pac
Encodes: puromycin-N-acetyl transferase
Source Database: Reslit
Confers Resistance To: Puromycin
Organisms Tested In: • Escherichia coli
Validation Method: functional testing in E. coli and HeLa cells

New selectable marker for manipulating the simple genomes of Mycoplasma species.

PMID 19687239 · 2009

The study describes the development of a new selectable marker, the pac gene encoding puromycin-N-acetyltransferase, which confers resistance to puromycin in multiple Mycoplasma species.

Gene Information

Gene Name: pac
Allele: pac
Encodes: puromycin-N-acetyltransferase
Source Database: Reslit
Mechanism: confers resistance to puromycin by enzymatic modification
Confers Resistance To: Puromycin
Organisms Tested In: • Mycoplasma mycoides subsp. mycoides
• Mycoplasma capricolum subsp. capricolum
• Mycoplasma genitalium
• Mycoplasma gallisepticum
• Mycoplasma pneumoniae
Validation Method: transformation experiments with puromycin selection
Sequence Accession: FJ872396|GQ420675|GQ420676

Mining Translation Inhibitors by a Unique Peptidyl-Aminonucleoside Synthetase Reveals Cystocin Biosynthesis and Self-Resistance.

PMID 39684615 · 2024

The study identifies the N-acetyltransferase CstC as a mediator of resistance to cystocin, demonstrating its ability to inactivate the antibiotic through N-acylation. CstC provides resistance to both cystocin and puromycin in bacterial and mammalian cells.

Gene Information

Gene Name: pac
Allele: pac
Encodes: protein
Source Database: Reslit
Confers Resistance To: Puromycin
Organisms Tested In: • Streptomyces sp. VKM Ac-502
• E. coli
Validation Method: transformation and MIC testing
Sequence Accession: JBHFFO000000000

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