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Explore antimicrobial resistance genes from the literature

ResLit

pbp5

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

UniProt

Protein entries specific to pbp5

AMR Rules

Browse AMR rules for pbp5

Overview

Drug Classes: BETA-LACTAM, Ampicillin, Penicillin
Organisms: Enterococcus faecium, Enterococcus faecalis
Papers: 15
Mutations: 43

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
N496K	-	penicillin-binding protein 5, Target modification	Enterococcus faecium	BETA-LACTAMAmpicillin	Reference Gene CatalogResFinder Database	Confirmed
E629V	-	penicillin-binding protein 5, Target modification	Enterococcus faecium, Enterococcus faecalis	AmpicillinBETA-LACTAM	Reference Gene CatalogResFinder DatabaseReslit

Papers15

1

Molecular characterization of ampicillin-resistant Enterococcus faecium isolates from hospitalized patients in Norway.

PMID 12791845 · 2003

pbp5

Mutation Information

pbp5: p.N496K pbp5: p.A499T

Protein Change: N496K, A499T
Organisms: Enterococcus faecium
Confers Resistance To: BETA-LACTAM
Effect on Function: penicillin-binding protein 5
Source Database: Reference Gene Catalog

2

The ionophore resistance genes narA and narB are geographically widespread and linked to resistance to medically important antibiotics.

PMID 40525834 · 2025

Mutation Information

pbp5: p.E629V

Protein Change: E629V
Organisms: Enterococcus faecium, Enterococcus faecalis
Confers Resistance To: Ampicillin
Source Database: Reslit

3

Global epidemiological insights on the antimicrobial resistance of Enterococcus faecium and a genomic analysis framework.

PMID 41264352 · 2025

Mutation Information

pbp5: p.M485A

Protein Change: M485A
Organisms: Enterococcus faecium
Confers Resistance To: Penicillin
Source Database: Reslit

4

Optimizing Nanopore Sequencing for Rapid Detection of Microbial Species and Antimicrobial Resistance in Patients at Risk of Surgical Site Infections.

PMID 35171692 · 2022

Detected in Enterococcus faecium

Mutation Information

pbp5: p.A111G pbp5: p.A192V

Protein Change: A111G, A192V
Confers Resistance To: PenicillinAmpicillin
Source Database: Reslit

5

Mechanisms of antibiotic resistance in enterococci.

PMID 25199988 · 2014

Mutation in Pbp5 leading to high-level ampicillin resistance

Mutation Information

pbp5: p.M485A

Protein Change: M485A
Organisms: Enterococcus faecium, Enterococcus faecalis
Confers Resistance To: Ampicillin
Source Database: Reslit

6

Characterization of a new transferable MDR plasmid carrying the pbp5 gene from a clade B commensal Enterococcus faecium.

PMID 30649343 · 2019

The PBP5 amino acid sequence differed from the C46 variant by two mutations (S39T and D644N).

Mutation Information

pbp5: p.D644N pbp5: p.S39T

Protein Change: D644N, S39T
Organisms: Enterococcus faecium
Confers Resistance To: Ampicillin
Source Database: Reslit

7

Diversification and Evolution of Vancomycin-Resistant Enterococcus faecium during Intestinal Domination.

PMID 31010813 · 2019

A nonsynonymous mutation in the gene for penicillin-binding protein 5 (pbp5) became fixed in the population by day 49.

pbp5

Mutation Information

pbp5: p.D434A

Protein Change: D434A
Organisms: Enterococcus faecium
Confers Resistance To: AmpicillinBETA-LACTAM
Effect on Function: penicillin-binding protein 5
Source Database: ReslitReference Gene Catalog

8

Genotyping using whole-genome sequencing is a realistic alternative to surveillance based on phenotypic antimicrobial susceptibility testing.

PMID 23233485 · 2013

pbp5

Mutation Information

pbp5: p.A401S pbp5: p.D325E pbp5: p.L610I

Protein Change: A401S, D325E, L610I
Organisms: Enterococcus faecium
Confers Resistance To: BETA-LACTAM
Effect on Function: penicillin-binding protein 5
Source Database: Reference Gene Catalog

9

Prediction of Antimicrobial Resistance in Clinical Enterococcus faecium Isolates Using a Rules-Based Analysis of Whole-Genome Sequences.

PMID 34694881 · 2022

pbp5

Mutation Information

pbp5: p.M485A pbp5: p.M485T

Protein Change: M485A, M485T
Organisms: Enterococcus faecium
Confers Resistance To: BETA-LACTAM
Effect on Function: penicillin-binding protein 5
Source Database: Reference Gene Catalog

10

Structure of the low-affinity penicillin-binding protein 5 PBP5fm in wild-type and highly penicillin-resistant strains of Enterococcus faecium.

PMID 8759860 · 1996

pbp5

Mutation Information

pbp5: p.A499T

Protein Change: A499T
Organisms: Enterococcus faecium
Confers Resistance To: BETA-LACTAM
Effect on Function: penicillin-binding protein 5
Source Database: Reference Gene Catalog

11

Antibiotic resistance determination using Enterococcus faecium whole-genome sequences: a diagnostic accuracy study using genotypic and phenotypic data.

PMID 38219758 · 2024

pbp5

Mutation Information

pbp5: p.V586L pbp5: p.M426I pbp5: p.E629V

Protein Change: V586L, M426I, E629V
Organisms: Enterococcus faecium
Confers Resistance To: BETA-LACTAM
Effect on Function: penicillin-binding protein 5
Source Database: Reference Gene Catalog

12

Whole-genome sequencing based characterization of antimicrobial resistance in Enterococcus.

PMID 29617860 · 2018

pbp5

Mutation Information

pbp5: p.D467DD pbp5: p.S466SS

Protein Change: D467DD, S466SS
Organisms: Enterococcus faecium
Confers Resistance To: BETA-LACTAM
Effect on Function: penicillin-binding protein 5
Source Database: Reference Gene Catalog

13

Modification of penicillin-binding protein 5 associated with high-level ampicillin resistance in Enterococcus faecium.

PMID 8834879 · 1996

pbp5

Mutation Information

pbp5: p.V586L pbp5: p.M426I

Protein Change: V586L, M426I
Organisms: Enterococcus faecium
Confers Resistance To: BETA-LACTAM
Effect on Function: penicillin-binding protein 5
Source Database: Reference Gene Catalog

14

Enterococcus faecium PBP5-S/R, the missing link between PBP5-S and PBP5-R.

PMID 25182648 · 2014

The nineteen pbp5 mutations must be present simultaneously for resistance phenotype

Not essential for resistance phenotype

Mutation Information

Protein Change: S27G, A68T, K144Q, D204G, M485A, E525D, P667S, ins466D, N496K, A499I, V586L, ins466S, V24A, G66E, E100Q, L177I, T324A, M485T, A499T, E629V, R34Q, E85D, T172A, A216S
Nucleotide Change: ins1396AGC, ins1396AGT, ins1396GAC, ins1396GAT, ins1396TCA, ins1396TCC, ins1396TCG, ins1396TCT
Organisms: Enterococcus faecium
Confers Resistance To: Ampicillin
Effect on Function: Target modification
Source Database: ResFinder Database

15

[The artificial feeding of tumor patients at home. Collaboration by the nutritional team and the community nursing service].

PMID 2518265 · 1989

The nineteen pbp5 mutations must be present simultaneously for resistance phenotype

Not essential for resistance phenotype

Mutation Information

pbp5: p.M485T pbp5: p.A499T pbp5: p.M485A pbp5: p.ins466D pbp5: p.A499I pbp5: p.ins466S pbp5: c.ins1396AGC pbp5: c.ins1396AGT pbp5: c.ins1396GAC pbp5: c.ins1396GAT pbp5: c.ins1396TCA pbp5: c.ins1396TCC pbp5: c.ins1396TCG pbp5: c.ins1396TCT

Protein Change: M485T, A499T, M485A, ins466D, A499I, ins466S
Nucleotide Change: ins1396AGC, ins1396AGT, ins1396GAC, ins1396GAT, ins1396TCA, ins1396TCC, ins1396TCG, ins1396TCT
Organisms: Enterococcus faecium
Confers Resistance To: Ampicillin
Effect on Function: Target modification
Source Database: ResFinder Database