Browse AMR Genes

Explore antimicrobial resistance genes from the literature

ptsI

Enzyme IIAGlc

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

Protein entries specific to ptsI

Browse AMR rules for ptsI

Validation StatusSupported

Overview

Mechanism: Lowers cAMP levels
Validation Method: Transposon insertion analysis
Drug Classes: Amikacin, Penicillin, Fosfomycin, Meropenem, Ceftriaxone, Ertapenem, Polymyxin b, Colistin, Gentamicin, Meropenem|Ceftriaxone|Ertapenem|Amikacin|Polymyxin b, FOSFOMYCIN, Trimethoprim-sulfamethoxazole
Organisms: Escherichia coli, Streptococcus gordonii, Klebsiella pneumoniae, Staphylococcus aureus
Geographic Location: Spain
Papers: 11
Databases: Reslit
Mutations: 7

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
V25I	-	single resistance variant, phosphoenolpyruvate--protein phosphotransferase PtsI	Escherichia coli	FosfomycinFOSFOMYCIN	Card DatabaseReference Gene CatalogReslit	Confirmed
I330P	-	-	Escherichia coli	Gentamicin	Reslit	Candidate
V488F

Papers11

1

Genetic architecture of intrinsic antibiotic susceptibility.

PMID 19462005 · 2009

The study identifies multiple genes and mutations that contribute to antibiotic tolerance in E. coli, revealing a large mutational target size for increasing drug resistance. Key findings include the role of genes involved in electron transport, flagella synthesis, and efflux pumps in modulating susceptibility to various antibiotics.

Gene Information

Gene Name: ptsI
Allele: ptsI
Encodes: Enzyme IIAGlc
Source Database: Reslit
Mechanism: Lowers cAMP levels
Confers Resistance To: Amikacin
Organisms Tested In: • Escherichia coli
Validation Method: Transposon insertion analysis

2

A single mutation in enzyme I of the sugar phosphotransferase system confers penicillin tolerance to Streptococcus gordonii.

PMID 19858257 · 2010

A single nucleotide mutation in the ptsI gene, which encodes enzyme I of the sugar phosphotransferase system, confers penicillin tolerance in Streptococcus gordonii. The mutation causes a proline-to-arginine substitution at position 483, reducing enzyme activity and carbohydrate uptake, thereby decreasing susceptibility to penicillin.

Gene Information

Gene Name: ptsI
Allele: ptsI
Encodes: enzyme I of the sugar phosphotransferase system
Source Database: Reslit
Mechanism: confers penicillin tolerance
Confers Resistance To: Penicillin
Organisms Tested In: • Streptococcus gordonii
Validation Method: mutagenesis and functional assays
Sequence Accession: DQ182633

3

Characterization of Fosfomycin and Nitrofurantoin Resistance Mechanisms in Escherichia coli Isolated in Clinical Urine Samples.

PMID 32847131 · 2020

The study identifies fosA3 as a novel plasmid-mediated fosfomycin resistance gene in E. coli isolates in Spain. Fosfomycin resistance is primarily due to defects in the UhpT transporter system, while nitrofurantoin resistance involves mutations in nfsA, nfsB, and ribE genes.

Gene Information

Gene Name: ptsI
Allele: ptsI
Encodes: phosphoenolpyruvate--protein phosphotransferase system
Source Database: Reslit
Confers Resistance To: Fosfomycin
Organisms Tested In: • Escherichia coli
Geographic Location: Spain
Validation Method: amino acid substitution analysis

4

In-patient evolution of a high-persister Escherichia coli strain with reduced in vivo antibiotic susceptibility.

PMID 38190524 · 2024

A loss-of-function mutation in the ptsI gene, encoding Enzyme I of the phosphoenolpyruvate phosphotransferase system, was identified in a high-persister Escherichia coli strain, leading to increased survival to multiple antibiotics in vivo.

Gene Information

Gene Name: ptsI
Allele: ptsI
Encodes: Phosphoenolpyruvate-protein phosphotransferase
Source Database: Reslit
Mechanism: Loss-of-function mutation leading to reduced antibiotic susceptibility
Confers Resistance To: MeropenemCeftriaxoneErtapenemAmikacinPolymyxin b
Organisms Tested In: • Escherichia coli
• Klebsiella pneumoniae
Validation Method: Growth curve analysis, complementation with wild-type ptsI, time-kill assay
Sequence Accession: PRJNA1007270

5

Membrane Proteins ClcB, PtsI, and YcaM Mediate the Bactericidal Effects of Colistin in Escherichia coli.

PMID 41165745 · 2025

The study identifies that the absence of membrane transporters ClcB, PtsI, and YcaM increases tolerance to colistin in E. coli, while their expression increases sensitivity to colistin. These proteins mediate the bactericidal effects of colistin.

Gene Information

Gene Name: ptsI
Allele: ptsI
Encodes: membrane transporter
Source Database: Reslit
Confers Resistance To: Colistin
Organisms Tested In: • Escherichia coli
Validation Method: growth assays, cytotoxic assays, in vivo infections