rfaE

hldE

Validation StatusCandidate

Overview

Mechanism: mutated in phage-resistant UPEC strains
Validation Method: whole-genome sequencing and growth assays
Drug Classes: Tigecycline
Organisms: Escherichia coli, Escherichia coli 536, Klebsiella species
Papers: 3
Databases: Reslit
Mutations: 1

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
T302P	-	-	Klebsiella species, Escherichia coli	Tigecycline	Reslit	Candidate

Papers3

Phage Resistance Accompanies Reduced Fitness of Uropathogenic Escherichia coli in the Urinary Environment.

PMID 35920561 · 2022

Phage-resistant UPEC strains exhibited mutations in lipopolysaccharide (LPS) biosynthesis genes, including rfaH, rfaI, waaO, and rfaE, which conferred resistance to phages HP3 and ES17.

Gene Information

Gene Name: rfaE
Allele: rfaE
Encodes: hldE
Source Database: Reslit
Mechanism: mutated in phage-resistant UPEC strains
Organisms Tested In: • Escherichia coli
Validation Method: whole-genome sequencing and growth assays

Variable fitness effects of bacteriophage resistance mutations in Escherichia coli: implications for phage therapy.

PMID 39213164 · 2024

The study identifies mutations in genes involved in lipopolysaccharide (LPS) biosynthesis and K15 capsule biosynthesis that confer resistance to phage 536_P1 in Escherichia coli 536. These mutations either modify the phage receptor or mask it via capsule overproduction.

Gene Information

Gene Name: rfaE
Allele: rfaE
Encodes: lipopolysaccharide biosynthesis
Source Database: Reslit
Mechanism: modification of phage receptor
Organisms Tested In: • Escherichia coli 536
Validation Method: trans-complementation experiment

Browse AMR Genes

rfaE

Mutations

Gene Information

Gene Information