rlmN

methyltransferase

Validation StatusCandidate

Overview

Validation Method: cloning in E. coli + primer extension and mass spectrometry
Drug Classes: Tiamulin, Linezolid|Quinupristin/dalfopristin
Organisms: Escherichia coli, Staphylococcus sciuri, Bacillus amyloliquefaciens, Bacillus clausii, Brevibacillus brevis, Clostridium sporogenes, Paenibacillus sp. Y412MC10, Thermus thermophilus, Bacillus subtilis, Deinococcus radiodurans, Arcobacter butzleri 55, E. coli, Staphylococcus aureus
Geographic Location: shellfish
Papers: 4
Databases: Reslit
Mutations: 2

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
Q89R	-	inhibits the endogenous RlmN C2 methylation of A2503	E. coli	Tiamulin	Reslit	Candidate
M1T	-	-	Staphylococcus aureus	Linezolid\|Quinupristin/dalfopristin	Reslit	Candidate

Papers4

Distinction between the Cfr Methyltransferase Conferring Antibiotic Resistance and the Housekeeping RlmN Methyltransferase

PMID 23752511 · 2013

The study distinguishes between the Cfr methyltransferase, which confers resistance to multiple antibiotics, and the RlmN methyltransferase, which does not significantly affect antibiotic resistance. Both are radical SAM enzymes but differ in their methylation sites and functional roles.

Gene Information

Gene Name: rlmN
Allele: rlmN
Encodes: methyltransferase
Source Database: Reslit
Organisms Tested In: • Escherichia coli
• Staphylococcus sciuri
• Bacillus amyloliquefaciens
• Bacillus clausii
• Brevibacillus brevis
• Clostridium sporogenes
• Paenibacillus sp. Y412MC10
• Thermus thermophilus
• Bacillus subtilis
• Deinococcus radiodurans
Validation Method: cloning in E. coli + primer extension and mass spectrometry

Genomic Characterization of Arcobacter butzleri Isolated From Shellfish: Novel Insight Into Antibiotic Resistance and Virulence Determinants.

PMID 31057492 · 2019

The study identified several antibiotic resistance genes in Arcobacter butzleri strains isolated from shellfish, including genes conferring resistance to beta-lactams, polymyxin, chloramphenicol, tetracycline, and macrolides.

Gene Information

Gene Name: rlmN
Allele: rlmN
Encodes: Dual-specificity RNA methyltransferase RlmN
Source Database: Reslit
Organisms Tested In: • Arcobacter butzleri 55
Geographic Location: shellfish
Validation Method: genomic analysis
Sequence Accession: QXMK00000000|QXNB0000000

Browse AMR Genes

rlmN

Mutations

Gene Information

Gene Information