The study identifies rmtF as a new 16S rRNA N7 G1405 methyltransferase gene responsible for high-level aminoglycoside resistance in Klebsiella pneumoniae BM4686.

The study presents SSTAR, a software tool for identifying antimicrobial resistance (AR) genes from whole-genome sequencing data. It detects known AR genes and potential new variants, including truncated forms. The tool was applied to analyze resistance genes in Klebsiella pneumoniae ST437 and Escherichia coli ST44, revealing various beta-lactamases, aminoglycoside resistance genes, and porin mutations contributing to resistance.

The paper discusses the rapid spread of carbapenem-resistant Enterobacteriaceae (CRE) and characterizes various carbapenemase genes such as bla KPC, bla NDM, bla VIM, bla OXA-48, and bla IMP, highlighting their roles in conferring resistance to carbapenems.

The study presents a rapid colorimetric test for detecting multiple aminoglycoside resistance in Enterobacteriaceae, focusing on the identification of 16S rRNA methylases (armA, rmtB, rmtC, rmtF, rmtG, npmA) and aminoglycoside-modifying enzymes (aac(3)-IV, aac(3)-Ia, aac(3)-V, aph(3')-I, aph(3')-Ib, ant(2'')).

The study identified the emergence of rmtC and rmtF 16S rRNA methyltransferase genes in clinical isolates of Pseudomonas aeruginosa, contributing to aminoglycoside resistance.

The study identified bla OXA-181, bla NDM, arr-3, aac (6′)-Ib-cr, rmtF, and catB1 as key resistance genes in colistin-resistant K. pneumoniae strains, highlighting the complex resistance mechanisms involving carbapenemases and aminoglycoside-modifying enzymes.

The study identified ten different 16S rRNA methyltransferase genes (rmtA, rmtB, rmtC, rmtD, rmtE, rmtF, rmtG, rmtH, armA, npmA) along with CTX-M-15, NDM, and OXA-48 beta-lactamases in three sequence types of Escherichia coli from northeast India.

Three ST15 Klebsiella pneumoniae isolates producing plasmid-mediated RmtF and OXA-232 were identified in China, highlighting the emergence of multidrug-resistant clones.

The study developed a targeted LC-MS/MS assay for the rapid and accurate detection of aminoglycoside-modifying enzymes and 16S rRNA methyltransferases in E. coli and K. pneumoniae, demonstrating high sensitivity and specificity for detecting resistance mechanisms to gentamicin, tobramycin, and amikacin.

The study identifies aminoglycoside-resistance genes such as aac(6')-Ib, aac(6')-Ib', aac(3)-II, aac(3)-IV, ant(2'')-I, aph(3')-I, and rmtF as significant predictors of aminoglycoside MICs in carbapenem-resistant Klebsiella pneumoniae.

The study identifies high rates of aminoglycoside methyltransferases (rmtB, armA, rmtF) and metallo-beta-lactamases (bla NDM, bla VIM) in multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa isolates from Egypt.

The study identifies various carbapenem resistance genes such as blaOXA232, blaNDM1, blaNDM5, blaOXA181, and others in Klebsiella pneumoniae isolates from India. It also characterizes mutations in ompK35 and ompK36 contributing to carbapenem resistance.

The study identifies multiple AMR genes and mutations in the pandrug-resistant K. pneumoniae strain DJ, including carbapenemases (bla NDM-5, bla OXA-181, bla CTX-M-15), aminoglycoside resistance genes (rmtB, rmtF, aac(6')-Ib, aadA2, strAB), sulfonamide resistance genes (sul1, sul2), dihydrofolate reductase (dfrA12), polymyxin resistance gene (mgrB), tetracycline resistance gene (ramR), chloramphenicol resistance genes (catA2, catB), fosfomycin resistance gene (fosA), and macrolide resistance genes (mphA, ermB). Mutations in gyrA, parC, ompK35, ompK36, and ramR contribute to resistance to fluoroquinolones, polymyxins, tetracyclines, and other antibiotics.

The study identified various aminoglycoside-modifying enzymes (AMEs) and efflux pump genes contributing to tobramycin resistance in E. coli, K. pneumoniae, and A. baumannii. These genes include acc(3)-IIa, aac(6')-Ib-cr, ant(2")-Ia, aph(6)-Id, aph(3")-Ib, armA, rmtF, acrD, adeA, adeB, adeC, ompA, omp37, csgB, csgD, csgF, csgG, pgaA, pgaB, pgaC, pgaD, csuA, csuB, csuC, csuD, csuE, and bap.

The study identified a high prevalence of 16S rRNA methylases (ArmA, RmtF, RmtB, RmtC, RmtG) and carbapenemases (NDM-1, NDM-5, KPC-2, KPC-3, OXA-48, OXA-181, OXA-232, VIM-1, VIM-2) in carbapenem- and aminoglycoside-resistant Enterobacterales isolates from Switzerland, highlighting the increasing trends of their association.

The study identified OXA-232-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) strains that exhibited multidrug resistance, including resistance to carbapenems, cephalosporins, aminoglycosides, and quinolones. The strains were part of a clonal spread within the ICU, showing genetic similarities and carrying resistance genes such as blaOXA-232, blaCTX-M-15, blaSHV-106, and others.

The study developed and validated a targeted LC-MS/MS assay for the rapid detection of β-lactam, aminoglycoside, and fluoroquinolone resistance mechanisms in blood cultures growing E. coli or K. pneumoniae. The assay successfully detected various resistance genes including β-lactamases (SHV, TEM, CTX-M-1-like, OXA-1, CMY-2-like, cAmpC, KPC, OXA-48, NDM, VIM), aminoglycoside-modifying enzymes (AAC(3)-Ia, AAC(3)-II, AAC(3)-IV, AAC(3)-VI, AAC(6′)-Ib, ANT(2′′)-I, APH(3′)-VI), 16S-RMTases (ArmA, RmtB, RmtC, RmtF), and quinolone resistance mechanisms (QnrA, QnrB, AAC(6′)-Ib-cr, and wildtype QRDR of GyrA).

The study identified multiple carbapenemase genes, including blaKPC-2, blaOXA-48-like, and blaNDM, along with 16S rRNA methyltransferases (armA, rmtF, rmtB) and extended-spectrum beta-lactamase blaCTX-M-15, contributing to carbapenem and aminoglycoside resistance in Klebsiella pneumoniae isolates from Singapore.

The study characterizes an OXA-232-producing ST15 CRKP strain with a multidrug-resistant profile, harboring chromosomal bla CTX-M-15 and plasmid-mediated bla OXA-232, along with other resistance genes such as strAB, rmtF, sul2, dfrA14, qnrB1, arr-2, and catB. Mutations in gyrA (S83F) and parC (S80I) contribute to fluoroquinolone resistance.

The study identifies the co-occurrence of bla OXA-232, rmtF, and a pLVPK-like virulence plasmid in a hypervirulent multidrug-resistant ST15-KL112 Klebsiella pneumoniae strain, highlighting the risks posed by the dissemination of these high-risk elements.

The study characterizes the AMR genes blaOXA-232 and rmtF in OXA-232-producing Klebsiella pneumoniae isolates in China, along with other resistance genes such as blaCTX-M-15, blaTEM-1B, aacA4'-17, aadA2, arr-2, qnrB1, and qnrS1. Mutations in gyrA and parC contribute to fluoroquinolone resistance.

The study identified 34 antimicrobial resistance genes (ARGs) in multidrug-resistant (MDR) plasmids from carbapenemase-producing Klebsiella pneumoniae clinical isolates in Pakistan, including bla NDM-1, bla OXA-48, and various beta-lactamases, aminoglycoside resistance genes, and others.

The study identified the presence of multiple AMR genes, including blaOXA-232, blaCTX-M-15, blaSHV-28, fosA, oqxA, oqxB, tet(E), AAC(6')-Ib, APH(3'')-Ib, APH(6)-Id, TEM-1, sul2, QnrB17, QnrB1, dfrA14, arr-2, AAC(6')-Ib9, and rmtF, in OXA-232-producing CRKP isolates from a hospital outbreak in China.

The study reports the first detection of blaNDM-1-positive Pseudomonas aeruginosa ST308 in Greece, highlighting the presence of multiple resistance genes including blaNDM-1, blaPAO, blaOXA-10, blaOXA-488, and others, indicating multidrug resistance.

The study identifies multiple AMR genes and mutations in Gram-negative bacteria causing neonatal sepsis in LMICs, including ESBLs, carbapenemases, and aminoglycoside-modifying enzymes, highlighting the high prevalence of multidrug resistance and the need for effective antibiotic combinations.

The study identifies various carbapenemase genes such as bla OXA-48, bla VIM-1, bla NDM-1, bla KPC-3, and bla NDM-5 in carbapenem-resistant E. coli isolates from Spain, highlighting their genetic diversity and geographic distribution.

The study identifies the co-harborance of bla(oxa-232) and rmtF in ST15-KL112 Klebsiella pneumoniae strains, highlighting their multidrug resistance to carbapenems and aminoglycosides.

The study identified the aminoglycoside resistance genes rmtF and aac(6')-Ib in carbapenemase-producing Enterobacteriaceae (CPE) strains, particularly in those carrying the bla OXA−48 gene. These genes were located on plasmids and contributed to amikacin resistance.

The study identifies the emergence of Klebsiella pneumoniae ST6260 harboring multiple AMR genes, including rmtF, rmtB, bla(NDM-5), bla(OXA-232), and bla(SFO-1), highlighting the complexity of resistance mechanisms in Enterobacterales.

The study identifies the emergence of Klebsiella pneumoniae ST6260 harboring multiple AMR genes, including rmtF, rmtB, bla(NDM-5), bla(OXA-232), and bla(SFO-1), highlighting the complexity of resistance mechanisms in Enterobacterales.

The study identified several AMR genes and mutations in colistin-resistant P. aeruginosa isolates, including blaNDM-1, blaOXA-1028, blaOXA-904, and mutations in phoQ and basR genes associated with colistin resistance.

The study characterizes the mobile genetic elements co-carrying bla NDM and 16S-RMTase genes in multiple Enterobacterales, highlighting the role of plasmids in the spread of these resistance genes in healthcare settings.

The study identifies 42 antimicrobial resistance (AMR) genes in the pan-drug resistant Klebsiella pneumoniae isolate KPNW, including beta-lactamases (bla CTX-M-15, bla NDM-1, bla OXA-1, bla TEM-63, bla TEM-104, bla SHV-28), tetracycline resistance genes (tet(A)), and efflux pump genes (oqxA, oqxB, marA, marR, ompK37, pbp3, crp, h-ns, kpnG, kpnH, parC, rsmA). Additionally, the isolate shows resistance to polymyxin B and colistin through modifications in lipid A (eptB, arnT, lptD, msbA, vanG) and other mechanisms.