slt

lytic transglycosylase

Validation StatusSupported

Overview

Mechanism: The paper states that loss of slt results in a two to fourfold decrease in MICs of piperacillin, cefotaxime, and ceftazidime, indicating that slt contributes to β-lactam resistance.
Validation Method: MIC testing
Drug Classes: Beta-lactams, Imipenem, Ceftazidime, Vancomycin
Organisms: Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO1, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa ID40
Papers: 5
Databases: Reslit
Mutations: 2

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
-	C1423T	-	-	Imipenem	Reslit	Candidate
E505A	G1514A	-	Escherichia coli	Vancomycin	Reslit	Candidate

Papers5

Loss of membrane-bound lytic transglycosylases increases outer membrane permeability and β-lactam sensitivity in Pseudomonas aeruginosa.

PMID 26374494 · 2015

The study identifies that loss of specific lytic transglycosylases (slt, mltB, and sltB1) in Pseudomonas aeruginosa increases β-lactam sensitivity, indicating their role in maintaining outer membrane integrity and reducing antibiotic resistance.

Gene Information

Gene Name: slt
Allele: slt
Encodes: lytic transglycosylase
Source Database: Reslit
Mechanism: The paper states that loss of slt results in a two to fourfold decrease in MICs of piperacillin, cefotaxime, and ceftazidime, indicating that slt contributes to β-lactam resistance.
Confers Resistance To: Beta-lactams
Organisms Tested In: • Pseudomonas aeruginosa
Validation Method: MIC testing

Slt, MltD, and MltG of Pseudomonas aeruginosa as Targets of Bulgecin A in Potentiation of β-Lactam Antibiotics.

PMID 30620575 · 2019

The study identifies Slt, MltD, and MltG as targets of bulgecin A, showing that their inhibition enhances the potency of β-lactam antibiotics by disrupting cell wall repair and promoting bulge formation and lysis in Pseudomonas aeruginosa.

Gene Information

Gene Name: slt
Allele: slt
Encodes: lytic transglycosylase
Source Database: Reslit
Mechanism: Inhibition by bulgecin A enhances β-lactam antibiotic potency by disrupting cell wall repair and causing bulge formation and subsequent lysis.
Organisms Tested In: • Pseudomonas aeruginosa PAO1
Validation Method: Transposon insertion mutants and swarm-motility assays demonstrating bulge formation and cell lysis in the presence of β-lactams and bulgecin A.

Chemogenomic Screen for Imipenem Resistance in Gram-Negative Bacteria.

PMID 31744905 · 2019

The study identified several genes and mutations associated with imipenem resistance in Gram-negative bacteria, including rpoD, amiC, nlpD, wecA, slt, and oprD, through mutagenesis and functional validation experiments.

Gene Information

Gene Name: slt
Allele: slt
Encodes: soluble lytic murein transglycosylase
Source Database: Reslit
Confers Resistance To: Imipenem
Organisms Tested In: • Escherichia coli
• Klebsiella pneumoniae
Validation Method: knock-in approach
Sequence Accession: PRJNA244551|PRJNA244567|PRJNA316664

Identification of Drug Resistance Determinants in a Clinical Isolate of Pseudomonas aeruginosa by High-Density Transposon Mutagenesis.

PMID 31818817 · 2020

The study identifies several nonessential genes involved in β-lactam resistance in Pseudomonas aeruginosa, including ampC, mltG, mepM1, amgK, and ygfB, which contribute to the induction of ampC expression and resistance against meropenem and cefepime.

Gene Information

Gene Name: slt
Allele: slt
Encodes: peptidoglycan hydrolase
Source Database: Reslit
Mechanism: peptidoglycan catabolite production
Confers Resistance To: Ceftazidime
Organisms Tested In: • Pseudomonas aeruginosa ID40
Validation Method: TraDIS screen, gene deletion
Sequence Accession: PRJEB32702

Lytic Transglycosylase Deficiency Increases Susceptibility to β-lactam Antibiotics But Reduces Susceptibility to Vancomycin in Escherichia coli.

PMID 40418177 · 2025

Gene Information

Gene Name: slt
Allele: slt
Encodes: lytic transglycosylase
Source Database: Reslit
Mechanism: loss of transglycosylase activity leads to susceptibility to β-lactam antibiotics and resistance to vancomycin
Confers Resistance To: Vancomycin
Organisms Tested In: • Escherichia coli
Validation Method: gene knockout and complementation with wild-type slt gene

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slt

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