The study identified multiple antibiotic resistance genes (ARGs) in carbapenem-resistant Klebsiella pneumoniae (CRKP) strains isolated from captive giant pandas, including aac C, bla CTX−M−04, bla OXY, bla SHV−01, bla SHV−02, amp C /bla DHA, tet B−01, tet B−02, tet C−01, tet C−02, and van TC−02. These genes were found to confer resistance to various antibiotics, highlighting the complexity of antimicrobial resistance in these strains.