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ResLit

tet(50)

tetracycline destructase Tet(50)

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

UniProt

Protein entries specific to tet(50)

AMR Rules

Browse AMR rules for tet(50)

Validation StatusConfirmed2+ DBs

Overview

Mechanism: Enzymatic inactivation
Validation Method: expression in E. coli and resistance assay
Drug Classes: TETRACYCLINE, Tetracycline
Organisms: uncultured bacterium, Escherichia coli, Legionella longbeachae
Alleles: 4
Papers: 6
Databases: Card DatabaseReference Gene CatalogResFinder DatabaseReslit

Allele Variants4

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
Tet(50)	Card DatabaseReference Gene CatalogResFinder DatabaseReslit	7	TETRACYCLINE, Tetracycline	uncultured bacterium +1	-	2015, 2017, 2019, 2023, 2024

Papers6

The Tetracycline Destructases: A Novel Family of Tetracycline-Inactivating Enzymes.

PMID 26097034 · 2015

Gene Information

Gene Name: tet(50)
Allele: Tet(50)
Encodes: tetracycline destructase Tet(50)
Source Database: Card Database
Mechanism: Enzymatic inactivation
Confers Resistance To: TETRACYCLINE
Organisms Tested In: • uncultured bacterium
Sequence Accession: KR857684.1
Protein Accession: AKQ05894.1
Notes: ARO:3004584

The Tetracycline Destructases: A Novel Family of Tetracycline-Inactivating Enzymes.

PMID 26097034 · 2015

Gene Information

Gene Name: tet(50)
Allele: tet(50)
Source Database: ResFinder Database
Mechanism: Enzymatic inactivation
Confers Resistance To: TETRACYCLINE
Organisms Tested In: • uncultured bacterium
Sequence Accession: KR857684

The Tetracycline Destructases: A Novel Family of Tetracycline-Inactivating Enzymes.

PMID 26097034 · 2015

Gene Information

Gene Name: tet(50)
Allele: Tet(50)
Encodes: tetracycline destructase Tet(50)
Source Database: Reference Gene Catalog
Mechanism: Enzymatic inactivation
Confers Resistance To: TETRACYCLINE
Organisms Tested In: • uncultured bacterium
Sequence Accession: KR857684.1
Protein Accession: AKQ05894.1
Notes: ARO:3004584

The Tetracycline Destructases: A Novel Family of Tetracycline-Inactivating Enzymes.

PMID 26097034 · 2015

Gene Information

Gene Name: tet(50)
Allele: Tet(50)
Encodes: tetracycline destructase Tet(50)
Source Database: ResFinder Database
Mechanism: Enzymatic inactivation
Confers Resistance To: TETRACYCLINE
Organisms Tested In: • uncultured bacterium
Sequence Accession: KR857684.1
Protein Accession: AKQ05894.1
Notes: ARO:3004584

Plasticity, dynamics, and inhibition of emerging tetracycline resistance enzymes.

PMID 28481346 · 2017

The study identifies and characterizes several tetracycline destructases, including tet(56), tet(X), tet(50), tet(51), and tet(55), which confer tetracycline resistance through enzymatic inactivation. These enzymes degrade tetracycline, leading to increased minimum inhibitory concentrations (MICs) in E. coli. Additionally, anhydrotetracycline is identified as an inhibitor of these enzymes, restoring tetracycline activity.

Gene Information

Gene Name: tet(50)
Allele: Tet(50)
Encodes: tetracycline destructase
Source Database: Reslit
Mechanism: enzymatic inactivation of tetracycline
Confers Resistance To: Tetracycline
Organisms Tested In: • Escherichia coli
Validation Method: expression in E. coli and resistance assay
Sequence Accession: NCBI Protein: WP_011221123|NCBI Protein: WP_011221124|NCBI Protein: WP_011221125|NCBI Protein: WP_011221126|NCBI Protein: WP_011221127|5TUE|5TUK|5TUL|5TUI|5TUF|5TUM

Tetracycline-Inactivating Enzymes.

PMID 29899733 · 2018

The paper reviews the structure, mechanism, and inhibition of tetracycline-destructase enzymes, highlighting their role in enzymatic inactivation of tetracyclines and the identification of new tetracycline destructase genes such as tetX, tetX1, tetX2, tet49, tet50, tet51, tet55, and tet56.

Gene Information

Gene Name: tet(50)
Allele: tet50
Encodes: tetracycline-inactivating enzyme
Source Database: Reslit
Confers Resistance To: Tetracycline
Organisms Tested In: • Legionella longbeachae
Validation Method: cloning, heterologous expression, and in vitro characterization
Sequence Accession: PDB:4a6n

Tetracycline-Inactivating Enzymes.

PMID 29899733 · 2018

Gene Information

Gene Name: tet(50)
Allele: Tet50
Encodes: tetracycline destructase
Source Database: Reslit
Confers Resistance To: Tetracycline
Organisms Tested In: • Escherichia coli
Validation Method: X-ray crystallography and biochemical assays
Sequence Accession: PDB:4a6n

Semisynthetic Analogues of Anhydrotetracycline as Inhibitors of Tetracycline Destructase Enzymes.

PMID 30835428 · 2019

The study characterizes the tetracycline destructase enzymes Tet(50), Tet(X), and Tet(X)_3, highlighting their roles in tetracycline inactivation and the development of anhydrotetracycline analogs as inhibitors.

Gene Information

Gene Name: tet(50)
Allele: Tet(50)
Encodes: tetracycline destructase enzyme
Source Database: Reslit
Confers Resistance To: Tetracycline
Organisms Tested In: • Escherichia coli
Validation Method: in vitro enzyme-dependent inactivation of tetracycline
Sequence Accession: KU547176.1

Structure-Based Design of Bisubstrate Tetracycline Destructase Inhibitors That Block Flavin Redox Cycling.

PMID 36877173 · 2023

The study identifies and characterizes novel bisubstrate inhibitors of tetracycline destructases (TDases), which are responsible for enzymatic inactivation of tetracycline antibiotics. These inhibitors effectively block the redox cycling of FAD, thereby preventing the inactivation of tetracyclines by TDases.

Gene Information

Gene Name: tet(50)
Allele: Tet(50)
Encodes: tetracycline destructase
Source Database: Reslit
Mechanism: inhibition of tetracycline destruction
Confers Resistance To: Tetracycline
Organisms Tested In: • Escherichia coli
Validation Method: in vitro TDase inhibition assays

Sequence-structure-function characterization of the emerging tetracycline destructase family of antibiotic resistance enzymes.

PMID 38493211 · 2024

The study identified 14 new tetracycline destructases (TDases) through HMM screening and functional validation, expanding the diversity of TDase sequences and providing insights into their sequence-structure-function relationships.

Gene Information

Gene Name: tet(50)
Allele: Tet(50)
Encodes: tetracycline deacylase
Source Database: Reslit
Confers Resistance To: Tetracycline
Organisms Tested In: • Escherichia coli
Validation Method: cloning in E. coli + MIC testing
Sequence Accession: 8TWG|8TWF

PMID N/A

Gene Information

Gene Name: tet(50)
Allele: tet(50)
Encodes: tetracycline inactivation enzyme
Source Database: Card Database
Mechanism: antibiotic inactivation
Organisms Tested In: • uncultured bacterium
Sequence Accession: KR857684.1
Protein Accession: AKQ05894.1
Notes: ARO:3004584