Browse AMR Genes

Explore antimicrobial resistance genes from the literature

uhpT

transporter for fosfomycin uptake

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

Protein entries specific to uhpT

Browse AMR rules for uhpT

Validation StatusSupported

Overview

Mechanism: deletion or nonsense mutation leading to loss of function
Validation Method: quantitative PCR and transport assay
Drug Classes: Fosfomycin, Ampicillin, ATM, Ceftiofur, Amoxicillin, CHL, TET, CIP, Sulfonamides, SXT, GEN, STR, Nalidixic acid, IPM, Polymyxin b, Fluoroquinolone, FOSFOMYCIN
Organisms: Escherichia coli, Staphylococcus aureus, Salmonella enterica, Klebsiella pneumoniae, Pseudomonas aeruginosa
Geographic Location: Bangladesh, Europe, Kuwait, Shanghai, Sichuan, China +1 more
Alleles: 2
Papers: 24
Databases: Reslit
Mutations: 43

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
W228Ter	-	nonsense mutation, hexose phosphate transporter UhpT	Staphylococcus aureus	FosfomycinFOSFOMYCIN	Card DatabaseReference Gene Catalog	Confirmed
G335A	-	-	Staphylococcus aureus	Fosfomycin	Reslit	Candidate
G683A	-	-

Allele Variants2

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
uhpT	Reslit	11	Fosfomycin, Ampicillin +14	Escherichia coli +4	Spain, Shanghai, Sichuan, China, Europe, Bangladesh	2015, 2018, 2020, 2022, 2023, 2025	SRX2676277\|SRX2676278\|SRX2676279\|SRX2676280\|SRX2676281\|SRX2676282\|SRX2676285\|SRX2676286\|SRX2676287\|SRX2676288	-
uhpT_E350Q	Reslit	1	Fosfomycin	Escherichia coli	Kuwait	2022	SRX8356292\|SRX8356293\|SRX8356294\|SRX8356295\|SRX8344629\|SRX8344630\|SRX8344631\|SRX8344632	-

Papers24

1

Elevated Expression of GlpT and UhpT via FNR Activation Contributes to Increased Fosfomycin Susceptibility in Escherichia coli under Anaerobic Conditions.

PMID 26248376 · 2015

The study shows that elevated expression of glpT and uhpT in anaerobic conditions increases fosfomycin susceptibility in E. coli by enhancing drug uptake.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: transporter for fosfomycin uptake
Source Database: Reslit
Confers Resistance To: Fosfomycin
Organisms Tested In: • Escherichia coli
Validation Method: quantitative PCR and transport assay

2

Frequency and Mechanisms of Spontaneous Fosfomycin Nonsusceptibility Observed upon Disk Diffusion Testing of Escherichia coli.

PMID 29093108 · 2018

The study identifies that fosfomycin-nonsusceptible inner colony mutants in E. coli arise due to the loss of function or induction of UhpT, primarily through deletions in uhpT or nonsense mutations in uhpA.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: hexose-6-phosphate antiporter
Source Database: Reslit
Mechanism: deletion or nonsense mutation leading to loss of function
Confers Resistance To: Fosfomycin
Organisms Tested In: • Escherichia coli
Validation Method: whole-genome sequencing, MIC testing, qRT-PCR
Sequence Accession: SRX2676277|SRX2676278|SRX2676279|SRX2676280|SRX2676281|SRX2676282|SRX2676285|SRX2676286|SRX2676287|SRX2676288

3

Characterization of Fosfomycin and Nitrofurantoin Resistance Mechanisms in Escherichia coli Isolated in Clinical Urine Samples.

PMID 32847131 · 2020

The study identifies fosA3 as a novel plasmid-mediated fosfomycin resistance gene in E. coli isolates in Spain. Fosfomycin resistance is primarily due to defects in the UhpT transporter system, while nitrofurantoin resistance involves mutations in nfsA, nfsB, and ribE genes.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: hexose phosphate transporter
Source Database: Reslit
Confers Resistance To: Fosfomycin
Organisms Tested In: • Escherichia coli
Geographic Location: Spain
Validation Method: gene deletion analysis

4

Whole Genome Sequence Analysis of Multidrug Resistant Escherichia coli and Klebsiella pneumoniae Strains in Kuwait.

PMID 35336083 · 2022

The study identified multiple AMR genes in multidrug-resistant E. coli and K. pneumoniae isolates from Kuwait, including beta-lactamases (blaKPC-2, blaCTX-M-15, blaOXA-1, blaCMY-4, blaTEM), aminoglycoside-modifying enzymes (aac(3)-IIa, aph(6)-Id, aadA5), sulfonamide resistance genes (sul1, sul2), quinolone resistance genes (gyrA_D87N, qnrB1), and others. Colistin resistance was linked to the pmrB_R256G mutation.

Gene Information

Gene Name: uhpT
Allele: uhpT_E350Q
Encodes: Fosfomycin resistance
Source Database: Reslit
Confers Resistance To: Fosfomycin
Organisms Tested In: • Escherichia coli
Geographic Location: Kuwait
Validation Method: whole genome sequencing
Sequence Accession: SRX8356292|SRX8356293|SRX8356294|SRX8356295|SRX8344629|SRX8344630|SRX8344631|SRX8344632

5

Mechanisms of high-level fosfomycin resistance in Staphylococcus aureus epidemic lineage ST5.

PMID 35848785 · 2022

High-level fosfomycin resistance in S. aureus ST5 lineage is mainly due to mutations in glpT and uhpT, whereas ST239 resistance is mainly due to mutations in hptA.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: membrane transporter
Source Database: Reslit
Confers Resistance To: Fosfomycin
Organisms Tested In: • Staphylococcus aureus
Geographic Location: Shanghai
Validation Method: gene deletion and complementation
Sequence Accession: PRJNA803935|PRJNA660290

6

Phenotypic and genotypic characterization of antimicrobial resistance profiles in Salmonella isolated from waterfowl in 2002-2005 and 2018-2020 in Sichuan, China.

PMID 36274743 · 2022

The study identified multiple AMR genes and mutations in Salmonella isolates from waterfowl in Sichuan, China, including beta-lactamases, aminoglycoside-modifying enzymes, tetracycline efflux pumps, and quinolone resistance genes. Mutations in gyrA and gyrB were associated with nalidixic acid resistance.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: glucose transporter
Source Database: Reslit
Confers Resistance To: AmpicillinATMCeftiofurAmoxicillinCHLTETCIPSulfonamidesSXTGENSTRNalidixic acidIPMPolymyxin b
Organisms Tested In: • Salmonella enterica
Geographic Location: Sichuan, China
Validation Method: whole-genome sequencing
Sequence Accession: PRJNA857101

7

Pervasive Selection for Clinically Relevant Resistance and Media Adaptive Mutations at Very Low Antibiotic Concentrations.

PMID 36627817 · 2023

The study identifies clinically relevant resistance mutations in E. coli under subMIC antibiotic concentrations, showing that mutations in glpT, uhpT, uhpC, uhpA, nfsA, nfsB, gyrA, gyrB, and envZ confer resistance to fosfomycin, nitrofurantoin, ciprofloxacin, and tetracycline.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: hexose-6 phosphate:phosphate antiporter
Source Database: Reslit
Mechanism: reduces fosfomycin uptake
Confers Resistance To: Fosfomycin
Organisms Tested In: • Escherichia coli
Validation Method: whole-genome sequencing

8

Uropathogenic Escherichia coli (UPEC)-Associated Urinary Tract Infections: The Molecular Basis for Challenges to Effective Treatment.

PMID 37764013 · 2023

This review discusses the molecular basis of challenges to effective treatment of UPEC-associated urinary tract infections, focusing on virulence factors and antibiotic resistance mechanisms.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: hexose phosphate transporter
Source Database: Reslit
Confers Resistance To: Fosfomycin
Organisms Tested In: • Escherichia coli
Validation Method: mutation analysis

9

Detection of Possible Resistance Mechanisms in Uropathogenic Escherichia coli Strains Isolated from Kidney Transplant Recipients Based on Whole Genome Sequencing.

PMID 40001563 · 2025

The study identifies mutations in the gyrA gene and the presence of the qnrS1 gene as key contributors to quinolone resistance in uropathogenic E. coli strains. Additionally, mutations in glpT, cyaA, and uhpT genes are associated with fosfomycin resistance.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: hexose phosphate transporter
Source Database: Reslit
Mechanism: chromosomal mutation leading to fosfomycin resistance
Confers Resistance To: Fosfomycin
Organisms Tested In: • Escherichia coli
Geographic Location: Europe
Validation Method: whole-genome sequencing and mutation analysis

10

Synergistic antibacterial activity and prevention of drug resistance of daptomycin combined with fosfomycin against methicillin-resistant Staphylococcus aureus.

PMID 40530996 · 2025

The study found that daptomycin and fosfomycin combination displayed synergistic antibacterial activity against MRSA and prevented the emergence of drug-resistant mutants. Specific mutations in mprF, cls2, uhpT, and murA genes were identified in single-drug resistant mutants, but no mutations were detected in the combination group.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: transporter protein
Source Database: Reslit
Mechanism: contributes to fosfomycin resistance
Confers Resistance To: Fosfomycin
Organisms Tested In: • Staphylococcus aureus
Validation Method: experimental validation in mutants
Sequence Accession: PQ298983|PQ299000|PQ285482|PQ285499|PQ299001|PQ299009

11

Genomic Characterization of Pan-Drug Resistant Klebsiella pneumoniae KPNW Isolated From UTI Patient in Bangladesh.

PMID 41025610 · 2025

The study identifies 42 antimicrobial resistance (AMR) genes in the pan-drug resistant Klebsiella pneumoniae isolate KPNW, including beta-lactamases (bla CTX-M-15, bla NDM-1, bla OXA-1, bla TEM-63, bla TEM-104, bla SHV-28), tetracycline resistance genes (tet(A)), and efflux pump genes (oqxA, oqxB, marA, marR, ompK37, pbp3, crp, h-ns, kpnG, kpnH, parC, rsmA). Additionally, the isolate shows resistance to polymyxin B and colistin through modifications in lipid A (eptB, arnT, lptD, msbA, vanG) and other mechanisms.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: Glucose-phosphate transporter
Source Database: Reslit
Mechanism: Reduced permeability
Confers Resistance To: Fluoroquinolone
Organisms Tested In: • Klebsiella pneumoniae
Geographic Location: Bangladesh
Validation Method: Whole genome sequencing and annotation
Sequence Accession: JAWJYM01|JAWJYM010000000.1|PRJNA1029536|SAMN37872047|SRR34563343|GCA_041928875.1

12

Multidrug-resistant Pseudomonas aeruginosa: Pathogenesis, resistance mechanisms, and novel therapeutic strategies.

PMID 41170650 · 2025

The paper discusses the multidrug resistance mechanisms of Pseudomonas aeruginosa, including beta-lactamases, aminoglycoside modifying enzymes, efflux pumps, and mutations in porin genes. It highlights the role of these mechanisms in antibiotic resistance and the challenges they pose in treating infections.

Gene Information

Gene Name: uhpT
Allele: uhpT
Encodes: hexose phosphate transporter
Source Database: Reslit
Confers Resistance To: Fosfomycin
Organisms Tested In: • Pseudomonas aeruginosa
Validation Method: experimental validation in this study