vanA

glycopeptide resistance

Overview

Drug Classes
vancomycin, teicoplanin
Organisms
Enterococcus faecium, Enterococcus faecium BM4147, Enterococcus faecalis +7 more
Papers
12
Papers12
1

vanA and vanB incorporate into an endemic ampicillin-resistant vancomycin-sensitive Enterococcus faecium strain: effect on interpretation of clonality.

PMID 10565910 · 1999

The study characterizes the incorporation of vanA and vanB genes into an endemic ampicillin-resistant vancomycin-sensitive Enterococcus faecium strain, highlighting their role in vancomycin resistance.

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
glycopeptide resistance
Validation Method
multiplex PCR and hybridization with vanA probe

Confers Resistance To

vancomycin

Organisms Tested In

Enterococcus faecium

Isolation Details

Country
Finland
2

The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA).

PMID 10908650 · 2000

The study elucidates the molecular basis of vancomycin resistance in Enterococci by characterizing the crystal structure of VanA, a d-alanyl-d-lactate ligase that plays a critical role in the biosynthesis of cell-wall precursors resistant to vancomycin.

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
d-alanyl-d-lactate ligase
Validation Method
crystal structure determination and enzymatic activity analysis

Mechanism

biosynthesis of alternate cell-wall precursors

Confers Resistance To

vancomycin

Organisms Tested In

Enterococcus faecium BM4147
3

Enterococcus faecalis resistant to vancomycin and teicoplanin (VanA phenotype) isolated from a bone marrow transplanted patient in Brazil.

PMID 11290314 · 2001

The study reports the first case of Enterococcus faecalis with the VanA phenotype resistant to vancomycin and teicoplanin in Brazil.

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
glycopeptide resistance
Validation Method
PCR and Etest

Confers Resistance To

vancomycinteicoplanin

Organisms Tested In

Enterococcus faecalis

Isolation Details

Country
Brazil
4

Antimicrobial Resistance Mechanisms and Molecular Detection Techniques

PMID 11585788 · 2023

The paper discusses molecular detection methods for antimicrobial resistance, focusing on genes like mecA and blaZ, which confer resistance to beta-lactam antibiotics in staphylococci.

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
d-Ala-d-Ala ligase
Validation Method
PCR and sequencing

Confers Resistance To

vancomycinteicoplanin

Organisms Tested In

Enterococcus faecium

Isolation Details

Country
Europe|North America|Asia
5

Excretion of vancomycin-resistant enterococci by wild mammals.

PMID 12023924 · 2002

Vancomycin-resistant Enterococcus faecium (VRE) were found in wild mammals, specifically woodmice and badgers, with the vanA gene being responsible for vancomycin resistance.

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
glycopeptide resistance
Validation Method
PCR and MIC testing

Confers Resistance To

vancomycin

Organisms Tested In

Enterococcus faecium
Apodemus sylvaticus
Clethrionomys glareolus
Meles meles

Isolation Details

Country
England
6

Impact of elements containing glycopeptide resistance genes on expression of virulence in Enterococcus faecalis peritonitis: a pilot study with rats.

PMID 12709322 · 2003

The study investigated the impact of glycopeptide resistance genes vanA and vanB on the virulence of Enterococcus faecalis in a rat peritonitis model. Both vanA and vanB conferred resistance to vancomycin.

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
glycopeptide resistance
Validation Method
susceptibility testing

Confers Resistance To

vancomycin

Organisms Tested In

Enterococcus faecalis
7

Presence of a vanA-carrying pheromone response plasmid (pBRG1) in a clinical isolate of Enterococcus faecium.

PMID 12709324 · 2003

The study identified a vanA-carrying pheromone response plasmid (pBRG1) in a clinical isolate of Enterococcus faecium, which confers high-level resistance to vancomycin and teicoplanin.

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
glycopeptide resistance determinant
Validation Method
plasmid analysis and hybridization

Confers Resistance To

vancomycinteicoplanin

Organisms Tested In

Enterococcus faecium
Enterococcus faecalis
8

Highly Conjugative pMG1-Like Plasmids Carrying Tn1546-Like Transposons That Encode Vancomycin Resistance in Enterococcus faecium.

PMID 14617670 · 2003

The study identifies vanA and Tn1546 as responsible for vancomycin resistance in Enterococcus faecium, highlighting their role in the dissemination of resistance through conjugative plasmids.

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
glycopeptide resistance determinant
Validation Method
PCR analysis and sequencing

Confers Resistance To

vancomycin

Organisms Tested In

Enterococcus faecium
Enterococcus avium

Isolation Details

Country
United States
9

Vancomycin-resistant Staphylococcus aureus isolate from a patient in Pennsylvania.

PMID 14693550 · 2004

The study reports the second VRSA isolate in the United States, which contains the vanA gene responsible for vancomycin resistance and other resistance genes such as mecA, erm(A), and aac(6')-aph(2").

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
glycopeptide resistance determinant
Validation Method
PCR and DNA sequence analysis

Confers Resistance To

vancomycin

Organisms Tested In

Staphylococcus aureus

Isolation Details

Country
Pennsylvania
10

Persistence of animal and human glycopeptide-resistant enterococci on two Norwegian poultry farms formerly exposed to avoparcin is associated with a widespread plasmid-mediated vanA element within a polyclonal enterococcus faecium population.

PMID 15640183 · 2005

The study identified a plasmid-mediated vanA element associated with glycopeptide-resistant Enterococcus faecium on Norwegian poultry farms, highlighting the persistence of resistance despite the ban on avoparcin.

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
glycopeptide resistance
Validation Method
PCR and hybridization analyses

Confers Resistance To

vancomycin

Organisms Tested In

Enterococcus faecium

Isolation Details

Country
Norway
11

First nosocomial outbreak of vancomycin-resistant Enterococcus faecium expressing a VanD-like phenotype associated with a vanA genotype.

PMID 16081891 · 2005

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
glycopeptide resistance
Validation Method
PCR and sequencing

Confers Resistance To

vancomycinteicoplanin

Organisms Tested In

Enterococcus faecium
12

Glycopeptide resistance vanA operons in Paenibacillus strains isolated from soil.

PMID 16189102 · 2005

The study identifies vanA operons in Paenibacillus strains that confer resistance to glycopeptide antibiotics such as vancomycin and teicoplanin. These operons show similarities to those found in enterococci.

Gene Information

Gene Name
vanA
Allele
VanA
Encodes
glycopeptide resistance
Validation Method
sequence analysis and enzymatic activity assays

Confers Resistance To

vancomycinteicoplanin

Organisms Tested In

Paenibacillus thiaminolyticus PT-2B1
Paenibacillus apiarius PA-B2B
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