Browse AMR Genes

Explore antimicrobial resistance genes from the literature

whiB7

WhiB-like and MDR effector gene

NCBI Pathogen Isolates

Bacterial genomes carrying this gene

NCBI Reference Gene Catalog

Known alleles and reference sequences

Protein entries specific to whiB7

Browse AMR rules for whiB7

Validation StatusSupported

Overview

Mechanism: efflux
Validation Method: RT-qPCR and MIC testing
Drug Classes: Ofloxacin, Amikacin, Clarithromycin, Aminoglycoside, Macrolide, Kanamycin, Tobramycin, Ethionamide
Organisms: Mycobacterium tuberculosis, Mycobacterium abscessus, Mycobacterium chelonae, Mycobacteroides abscessus
Geographic Location: Argentina, China, India, global|Global
Alleles: 2
Papers: 11
Databases: Reslit
Mutations: 11

Mutations

Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
A51T	-	-	Mycobacterium abscessus	-	Reslit	Candidate
R14S	-	-	-	Kanamycin	Reslit	Candidate
R85C	-	-	-	Kanamycin	Reslit	Candidate

Allele Variants2

Allele	Database	Papers	Drug Classes	Organisms	Countries	Years	Sequence Accession	Protein Accession
whiB7	Reslit	9	Ofloxacin, Amikacin +6	Mycobacterium tuberculosis +3	China, Argentina, India, global\|Global	2011, 2017, 2019, 2020, 2021, 2022	PRJNA646920	-
WhiB7	Reslit	1	-	Mycobacterium abscessus	-	2022	PRJNA655109\|GSE163621	-

Papers11

1

Rifampicin reduces susceptibility to ofloxacin in rifampicin-resistant Mycobacterium tuberculosis through efflux.

PMID 21512166 · 2011

Rifampicin-resistant Mycobacterium tuberculosis strains exhibit reduced susceptibility to ofloxacin through the activation of efflux pumps. Specific efflux-related genes such as Rv3239c, Rv1258c, Rv2333c, Rv1877, Rv2994, Rv1634, Rv1747, whiB7, and Rv1002c were upregulated upon rifampicin exposure, contributing to ofloxacin resistance.

Gene Information

Gene Name: whiB7
Allele: whiB7
Encodes: WhiB-like and MDR effector gene
Source Database: Reslit
Mechanism: efflux
Confers Resistance To: Ofloxacin
Organisms Tested In: • Mycobacterium tuberculosis
Validation Method: RT-qPCR and MIC testing

2

Antagonism between Front-Line Antibiotics Clarithromycin and Amikacin in the Treatment of Mycobacterium abscessus Infections Is Mediated by the whiB7 Gene.

PMID 28874379 · 2017

The study identifies erm(41) and eis2 as genes involved in clarithromycin and amikacin resistance in Mycobacterium abscessus, mediated by the whiB7 gene.

Gene Information

Gene Name: whiB7
Allele: whiB7
Encodes: transcriptional regulator
Source Database: Reslit
Confers Resistance To: AmikacinClarithromycin
Organisms Tested In: • Mycobacterium abscessus
Validation Method: gene disruption and complementation experiments

3

Mycobacterium abscessus, an Emerging and Worrisome Pathogen among Cystic Fibrosis Patients.

PMID 31766758 · 2019

The paper discusses various AMR genes and mutations in Mycobacterium abscessus, including erm(41) for macrolide resistance, aac(2')-Ib for aminoglycoside resistance, blaMab for beta-lactam resistance, mabTetX for tetracycline resistance, rrs and rpsL for aminoglycoside resistance, mmpL3 for capuramycin resistance, and whiB7 for regulating resistance to aminoglycosides and macrolides.

Gene Information

Gene Name: whiB7
Allele: whiB7
Encodes: transcriptional regulator
Source Database: Reslit
Confers Resistance To: AminoglycosideMacrolide
Organisms Tested In: • Mycobacterium abscessus
Validation Method: experimental validation

4

Efflux Pumps Contribute to Intrinsic Clarithromycin Resistance in Clinical, Mycobacterium abscessus Isolates.

PMID 32104016 · 2020

Efflux pumps MAB_2355c, MAB_1409c, and MAB_1846 contribute to intrinsic clarithromycin resistance in Mycobacterium abscessus, alongside the regulatory gene whiB7.

Gene Information

Gene Name: whiB7
Allele: whiB7
Encodes: Positive regulatory gene
Source Database: Reslit
Mechanism: regulation of efflux pump expression
Confers Resistance To: Clarithromycin
Organisms Tested In: • Mycobacterium abscessus
Geographic Location: China
Validation Method: qRT-PCR

5

Five-year microevolution of a multidrug-resistant Mycobacterium tuberculosis strain within a patient with inadequate compliance to treatment.

PMID 33926375 · 2021

The study identifies several mutations in Mycobacterium tuberculosis that confer resistance to various antibiotics, including isoniazid, rifampicin, streptomycin, fluoroquinolones, pyrazinamide, ethionamide, bedaquiline, clofazimine, and kanamycin. These mutations were found to evolve over five years of treatment in a patient with inadequate compliance.

Gene Information

Gene Name: whiB7
Allele: whiB7
Encodes: Transcriptional activator of eis gene
Source Database: Reslit
Mechanism: Mutation in 5' untranslated region leads to resistance
Confers Resistance To: Kanamycin
Organisms Tested In: • Mycobacterium tuberculosis
Geographic Location: Argentina
Validation Method: Whole genome sequencing and phenotypic drug susceptibility testing
Sequence Accession: PRJNA646920

6

Increased whiB7 expression and antibiotic resistance in Mycobacterium chelonae carrying two prophages.

PMID 34107872 · 2021

The study identifies whiB7 as a key gene involved in increased antibiotic resistance in Mycobacterium chelonae carrying two prophages, McProf and BPs. The presence of these prophages leads to upregulation of the whiB7 regulon, contributing to resistance against amikacin and tobramycin.

Gene Information

Gene Name: whiB7
Allele: whiB7
Encodes: transcriptional regulator
Source Database: Reslit
Confers Resistance To: AmikacinTobramycin
Organisms Tested In: • Mycobacterium chelonae
Validation Method: qPCR and RNAseq analysis
Sequence Accession: GSE164210

7

RNA Sequencing Elucidates Drug-Specific Mechanisms of Antibiotic Tolerance and Resistance in Mycobacterium abscessus.

PMID 34633851 · 2022

The study identifies several genes involved in antibiotic resistance in Mycobacterium abscessus, including erm(41) for macrolide resistance, WhiB7 for general resistance to ribosome-targeting antibiotics, sigH for tigecycline resistance, cydA for clofazimine tolerance, and various aminoglycoside-modifying enzymes like aac(2'), aph(3'), and eis2.

Gene Information

Gene Name: whiB7
Allele: WhiB7
Encodes: transcriptional regulator
Source Database: Reslit
Organisms Tested In: • Mycobacterium abscessus
Validation Method: RNA sequencing and transcriptomic analysis
Sequence Accession: PRJNA655109|GSE163621

8

Molecular Epidemiology and Polymorphism Analysis in Drug-Resistant Genes in M. tuberculosis Clinical Isolates from Western and Northern India.

PMID 35422638 · 2022

The study identified several mutations in the rrs, eis, whiB7, tlyA, and gyrA genes associated with resistance to kanamycin, capreomycin, and ofloxacin in Mycobacterium tuberculosis clinical isolates from western and northern India.

Gene Information

Gene Name: whiB7
Allele: whiB7
Encodes: transcriptional regulator
Source Database: Reslit
Confers Resistance To: Kanamycin
Organisms Tested In: • Mycobacterium tuberculosis
Geographic Location: India
Validation Method: DNA sequencing
Sequence Accession: MN131053-MN131064|MN166763-MN166777

9

Genetic Determinants of Tigecycline Resistance in Mycobacteroides abscessus.

PMID 35625216 · 2022

The study identifies WhiB7, SigH, and RshA as genetic determinants associated with tigecycline resistance or reduced susceptibility in Mycobacteroides abscessus. Mutations in rshA and sigH, as well as the deletion of whiB7, contribute to tigecycline resistance through various mechanisms involving stress response pathways.

Gene Information

Gene Name: whiB7
Allele: whiB7
Encodes: transcriptional activator
Source Database: Reslit
Mechanism: reduced tigecycline susceptibility
Organisms Tested In: • Mycobacteroides abscessus
Validation Method: deletion of the WhiB7-encoding gene caused a 4-fold decrease in the minimum inhibitory concentration (MIC) of tigecycline

10

Genome-wide association studies of global Mycobacterium tuberculosis resistance to 13 antimicrobials in 10,228 genomes identify new resistance mechanisms.

PMID 35944070 · 2022

The study identified several novel resistance mechanisms in Mycobacterium tuberculosis through genome-wide association studies, including new genes and mutations associated with resistance to various antimicrobials such as ethambutol, isoniazid, amikacin, kanamycin, ethionamide, levofloxacin, rifabutin, clofazimine, delamanid, and linezolid.

Gene Information

Gene Name: whiB7
Allele: whiB7
Encodes: transcriptional regulator
Source Database: Reslit
Confers Resistance To: Ethionamide
Organisms Tested In: • Mycobacterium tuberculosis
Geographic Location: global|Global
Validation Method: genome-wide association study
Sequence Accession: GCA_000000000.1