ycbB

L,D-transpeptidase

Validation StatusCandidate

Overview

Mechanism: bypass of the D,D-transpeptidase activity of PBPs
Validation Method: plasmid-based expression and disk diffusion assay
Drug Classes: Ampicillin, Ceftriaxone
Organisms: Escherichia coli
Papers: 2
Databases: Reslit

Papers2

Factors essential for L,D-transpeptidase-mediated peptidoglycan cross-linking and β-lactam resistance in Escherichia coli.

PMID 27767957 · 2016

The study shows that the L,D-transpeptidase YcbB can bypass the D,D-transpeptidase activity of penicillin-binding proteins (PBPs) in E. coli, leading to broad-spectrum β-lactam resistance. This resistance requires the overproduction of (p)ppGpp and a chromosomal mutation.

Gene Information

Gene Name: ycbB
Allele: ycbB
Encodes: L,D-transpeptidase
Source Database: Reslit
Mechanism: bypass of the D,D-transpeptidase activity of PBPs
Confers Resistance To: AmpicillinCeftriaxone
Organisms Tested In: • Escherichia coli
Validation Method: plasmid-based expression and disk diffusion assay

Role of endopeptidases in peptidoglycan synthesis mediated by alternative cross-linking enzymes in Escherichia coli.

PMID 34382698 · 2021

The study identifies mepM and mepK as essential endopeptidases for β-lactam resistance mediated by the YcbB L,D-transpeptidase in Escherichia coli. These genes are crucial for hydrolyzing both 4→3 and 3→3 cross-links in peptidoglycan, facilitating resistance to ceftriaxone.

Gene Information

Gene Name: ycbB
Allele: ycbB
Encodes: L,D-transpeptidase
Source Database: Reslit
Mechanism: mediates β-lactam resistance by forming 3→3 cross-links in peptidoglycan
Confers Resistance To: Ceftriaxone
Organisms Tested In: • Escherichia coli
Validation Method: plasmid-based expression and disk diffusion assay
Sequence Accession: PRJNA748867

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ycbB

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