mecA
penicillin-binding protein 2'
Overview
- Drug Classes
- methicillin, oxacillin, beta-lactam, cephalothin, cefazolin, cefoxitin, cloxacillin, dicloxacillin, nafcillin
- Organisms
- Staphylococcus aureus, coagulase-negative Staphylococcus spp., Staphylococcus epidermidis +13 more
- Alleles
- 2
- Papers
- 11
| Allele | Papers | Drug Classes | Organisms | Countries | Years | Status |
|---|---|---|---|---|---|---|
| MecA | 10 | methicillin, oxacillin +7 | Staphylococcus aureus +15 | Portugal|USA|Japan|Poland|Hungary|Denmark, Europe|North America|Asia, Pennsylvania, Canada | 1999, 2000, 2003, 2004, 2005, 2023 | Not Validated |
| mec | 1 | methicillin | Staphylococcus aureus | Ireland | 2005 | Not Validated |
Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315.
PMID 10348769 · 1999The study identifies the mecA gene, which encodes a low-affinity penicillin-binding protein responsible for methicillin resistance in Staphylococcus aureus. The gene is part of the mec DNA, which is integrated into the chromosome of pre-methicillin-resistant S. aureus N315.
Gene Information
- Gene Name
- mecA
- Allele
- MecA
- Encodes
- penicillin-binding protein 2'
- Validation Method
- cloning and sequence analysis
Mechanism
chromosomal gene encoding a low-affinity penicillin-binding protein that confers methicillin resistance
Confers Resistance To
Organisms Tested In
Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp.
PMID 10449481 · 1999The study evaluates various susceptibility testing methods for detecting oxacillin resistance in Staphylococcus aureus and coagulase-negative Staphylococcus spp., highlighting the importance of the mecA gene in mediating resistance.
Gene Information
- Gene Name
- mecA
- Allele
- MecA
- Encodes
- a membrane-bound penicillin-binding protein (PBP2a) that has low affinity for beta-lactam antibiotics
- Validation Method
- PCR detection of the mecA gene
Confers Resistance To
Organisms Tested In
A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus.
PMID 10817707 · 2000The study identifies the mecA gene, which encodes a low-affinity penicillin-binding protein responsible for methicillin resistance in Staphylococcus aureus. Additionally, the ccrA and ccrB genes, which encode site-specific recombinases, were found to mediate the integration and excision of the SCC mec element, a novel genetic element associated with methicillin resistance.
Gene Information
- Gene Name
- mecA
- Allele
- MecA
- Encodes
- penicillin-binding protein
- Validation Method
- cloning and expression in S. aureus, demonstrating reduced susceptibility to methicillin
Mechanism
production of a low-affinity penicillin-binding protein (PBP 2′) that is not inhibited by β-lactam antibiotics
Confers Resistance To
Organisms Tested In
Rapid detection of mecA-positive and mecA-negative coagulase-negative staphylococci by an anti-penicillin binding protein 2a slide latex agglutination test.
PMID 10834952 · 2000The study evaluated the MRSA-Screen latex agglutination test for detecting mecA-positive coagulase-negative staphylococci, demonstrating high sensitivity and specificity for methicillin resistance.
Gene Information
- Gene Name
- mecA
- Allele
- MecA
- Encodes
- penicillin-binding protein 2a
- Validation Method
- PCR and oxacillin MIC testing
Confers Resistance To
Organisms Tested In
Genetic organization of the downstream region of the mecA element in methicillin-resistant Staphylococcus aureus isolates carrying different polymorphisms of this region.
PMID 10858352 · 2000The study characterizes the genetic organization of the mecA element in methicillin-resistant Staphylococcus aureus isolates, focusing on the downstream region and identifying variations in the genetic structure associated with different polymorphisms of the mecA gene.
Gene Information
- Gene Name
- mecA
- Allele
- MecA
- Encodes
- penicillin-binding protein 2a (PBP2a)
- Validation Method
- cloning and sequencing of the downstream region of the mecA element
Mechanism
chromosomal gene encoding a modified penicillin-binding protein that confers resistance to β-lactam antibiotics
Confers Resistance To
Organisms Tested In
Isolation Details
Antimicrobial Resistance Mechanisms and Molecular Detection Techniques
PMID 11585788 · 2023The paper discusses molecular detection methods for antimicrobial resistance, focusing on genes like mecA and blaZ, which confer resistance to beta-lactam antibiotics in staphylococci.
Gene Information
- Gene Name
- mecA
- Allele
- MecA
- Encodes
- penicillin-binding protein
- Validation Method
- molecular detection
Mechanism
methicillin resistance
Confers Resistance To
Organisms Tested In
Isolation Details
Jumping the barrier to beta-lactam resistance in Staphylococcus aureus.
PMID 12949098 · 2003The study identifies mecA as a gene that confers beta-lactam resistance in Staphylococcus aureus by encoding PBP2a, a penicillin-binding protein that is resistant to beta-lactam antibiotics. The research shows that the expression of mecA is regulated by mecR1-mecI and that the presence of regulatory genes influences the stability and expression of mecA in different genetic backgrounds.
Gene Information
- Gene Name
- mecA
- Allele
- MecA
- Encodes
- penicillin-binding protein 2a
- Validation Method
- expression in S. aureus strains and detection of PBP2a via Western blot
Mechanism
confers resistance to beta-lactam antibiotics by altering the target site
Confers Resistance To
Organisms Tested In
Vancomycin-resistant Staphylococcus aureus isolate from a patient in Pennsylvania.
PMID 14693550 · 2004The study reports the second VRSA isolate in the United States, which contains the vanA gene responsible for vancomycin resistance and other resistance genes such as mecA, erm(A), and aac(6')-aph(2").
Gene Information
- Gene Name
- mecA
- Allele
- MecA
- Encodes
- methicillin resistance determinant
- Validation Method
- PCR and DNA sequence analysis
Confers Resistance To
Organisms Tested In
Isolation Details
New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci.
PMID 15528678 · 2004The study developed a quadriplex PCR assay to detect methicillin and mupirocin resistance in Staphylococcus aureus and coagulase-negative staphylococci, targeting the mecA and mupA genes.
Gene Information
- Gene Name
- mecA
- Allele
- MecA
- Encodes
- low-affinity penicillin-binding protein (PBP2a) that confers resistance to methicillin
- Validation Method
- PCR assay for the mecA gene
Confers Resistance To
Organisms Tested In
Seven novel variants of the staphylococcal chromosomal cassette mec in methicillin-resistant Staphylococcus aureus isolates from Ireland.
PMID 15855533 · 2005The study identified seven novel variants of the staphylococcal chromosomal cassette mec (SCC mec) in methicillin-resistant Staphylococcus aureus (MRSA) isolates from Ireland. These variants include SCC mec IIA, IIB, IIC, IID, IIE, IVE, and IVF, which exhibit distinct genomic structures and differences in the mec complex and associated regions.
Gene Information
- Gene Name
- mecA
- Allele
- mec
- Encodes
- beta-lactam resistance determinant
- Validation Method
- PCR and sequencing
Confers Resistance To
Organisms Tested In
Isolation Details
Community-associated methicillin-resistant Staphylococcus aureus, Canada.
PMID 15963278 · 2005The study identified two major clonal groups of methicillin-resistant Staphylococcus aureus (MRSA) in Saskatchewan, Canada. Clonal group A was associated with a long-term care facility and exhibited multidrug resistance, while clonal group B was community-associated and showed susceptibility to most antimicrobial agents except for high-level mupirocin resistance. Both groups harbored the mecA gene, which confers resistance to beta-lactam antibiotics.
Gene Information
- Gene Name
- mecA
- Allele
- MecA
- Encodes
- penicillin-binding protein 2a
- Validation Method
- PCR and PFGE
Confers Resistance To
Organisms Tested In
Isolation Details
No comments yet. Be the first to comment!