Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance.

PMID: 350156302022

Key Findings

Directed evolution of the Cfr enzyme led to variants that enhance resistance to multiple classes of ribosome-targeting antibiotics by increasing rRNA methylation at A2503.

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1gene entry

3mutation entries

AMR Genes

Gene Name	Alleles	Encodes	Confers Resistance To	Organisms	Database	Validation Status
cfr	1	rRNA methylating enzyme	PleuromutilinPhenicolLincosamideOxazolidinoneStreptogramin a	Escherichia coli	Reslit	-

AMR Mutations

Gene	Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
cfr	S39G	-	-	E. coli	Hygromycin\|Tiamulin	Reslit	-
cfr	N2K	-	-	E. coli	Hygromycin\|Tiamulin	Reslit	-
cfr	I26M	-	-	E. coli	Hygromycin\|Tiamulin	Reslit

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Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance.

AMR Genes

AMR Mutations