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Beyond Hotspot Mutations: Diagnostic Relevance of High Frequency, Low Frequency, and Disputed rpoB Variants in Rifampicin-Resistant Mycobacterium tuberculosis.

PMID: 415990002025

Key Findings

Mutations in rpoB are responsible for rifampicin resistance in Mycobacterium tuberculosis.

0gene entries

10mutation entries

AMR Mutations

Gene	Protein Change	Nucleotide Change	Mechanism	Organism	Resistance To	Database	Validation Status
rpoB	H445Y	-	disrupts an intermolecular hydrogen bond between histidine and rifampicin and introduces an aromatic ring that sterically interferes with rifampicin binding	Mycobacterium tuberculosis	Rifampicin	Reslit	-
rpoB	H445N	-	-	Mycobacterium tuberculosis	Rifampicin	Reslit	-
rpoB	H445D	-	replaces a neutral histidine with a negatively charged residue, weakening hydrogen bonding and destabilizing drug interactions	Mycobacterium tuberculosis	Rifampicin	Reslit	-
rpoB	H445L	-	-	Mycobacterium tuberculosis	Rifampicin	Reslit	-
rpoB	D435V	-	substitutes the negatively charged aspartate with a bulkier nonpolar valine residue causing steric hindrance within the rifampicin-binding pocket	Mycobacterium tuberculosis	Rifampicin	Reslit	-
rpoB	L452P	-	-	Mycobacterium tuberculosis	Rifampicin	Reslit	-
rpoB	L430P	-	-	Mycobacterium tuberculosis	Rifampicin	Reslit	-
rpoB	I491F	-	-	Mycobacterium tuberculosis	Rifampicin	Reslit	-
rpoB	S450L	-	disrupts a key intermolecular hydrogen bond between serine and RIF and introduces a hydrophobic leucine residue that causes steric hindrance	Mycobacterium tuberculosis	Rifampicin	Reslit	-
rpoB	D435Y	-	-	Mycobacterium tuberculosis	Rifampicin	Reslit	-