Key Findings

The study identified mutations in the vanE operon and gyrB gene of Enterococcus faecalis BM4405-1, which led to silencing of glycopeptide resistance. The vanE operon, including vanE, vanXYE, and vanTE, was found to confer vancomycin resistance when cloned into a susceptible strain. The mutation in gyrB (K337Y) was linked to altered DNA supercoiling and reduced expression of the vanE operon.